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193149-74-5

2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt

CAS: 193149-74-5

Molecular Formula: C20H15N6NaO11S2

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193149-74-5 - Names and Identifiers

Name 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt
Synonyms WST8
WST 8
GLT008
SODIUM
Cell Proliferation Assay
2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophen
4-[2-(2-METHOXY-4-NITROPHENYL)-3-(4-NITROPHENYL)TETRAZOL-2-IUM-5-YL]BENZENE-1,3-DISULFONATE
2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt
2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt
CAS 193149-74-5

193149-74-5 - Physico-chemical Properties

Molecular FormulaC20H15N6NaO11S2
Molar Mass602.48
Storage Conditionunder inert gas (nitrogen or Argon) at 2-8°C
UseScreening of Cell Proliferation Detects
In vitro study The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×10 5 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line.

193149-74-5 - Reference

Reference
Show more
1. Zhang, Zhe, et al. "Lactobacillus casei YRL577 combined with plant extracts reduce markers of non-alcoholic fatty liver disease in mice." British Journal of Nutrition 125.10 (2021): 1081-1091https://doi.org/10.1017/S0007114520003013
2. Li, Guangzhang, et al. "miR‐505 inhibits proliferation of osteosarcoma via HMGB1." FEBS open bio 10.7 (2020): 1251-1260.https://doi.org/10.1002/2211-5463.12868

193149-74-5 - Introduction

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM.
Last Update：2022-10-16 17:14:42

193149-74-5 - Reference Information

Overview 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfobenzene)-2H-tetrazole monosodium salt, referred to as WST-8, is a main ingredient in the cell activity detection agent CCK-8 kit. CCK-8 kit is a kit developed by Japan Dojindo Research Institute to detect cell proliferation and cytotoxicity. The kit is easy to use, high sensitivity, good repeatability and no radioactivity. However, the kit is expensive and its main ingredients are WST-8 sold in the market.
Use 2-(2-methoxy-4-nitrobenzene)-3-(4-nitrobenzene)-5-(2, 4-disulfobenzene)-2H-tetrazole monosodium salt is a highly sensitive tetrazole reagent that can produce water-soluble A in NADH. It is a new generation of methyl maz-based dyes that release the conversion products into the culture medium in a soluble form, allowing non-destructive determination of viability, allowing cells to accept further investigation.
Screening of Cell Proliferation Detector
Application 2-(2-methoxy-4-nitrobenzene)-3-(4-nitrobenzene)-5-(2, 4-disulfobenzene)-2H-tetrazole monosodium salt is mainly used in biochemical kits as a screening reagent for cell proliferation detection.
biological activity WST-8 is a water-soluble tetrazolium salt, which can be used to evaluate the metabolic activity of cells and produce the corresponding methyl dye, which is absorbed at 460 nm. WST-8 is usually used as an indicator of cell viability in cell proliferation assays.
Last Update：2024-04-09 19:05:10
193149-74-5
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CAS: 193149-74-5
Tel: 18301782025
Email: 3008007409@qq.com
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Nantong Reform Petro-Chemical CO., LTD.
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Product Name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt Request for quotation
CAS: 193149-74-5
Tel: +86-17551318830
Email: r@reformchem.com­
Mobile: +86-17551318830
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Jiangsu Pules Biotechnology Co.,Ltd.
Product Name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt Request for quotation
CAS: 193149-74-5
Tel: 17505222756
Email: pules.cn@gmail.com
Mobile: +86-17551318830
Wechat: 17505222756
Wuhan Boye Science And Technology Development Co., Ltd.
Product Name: WST-8 Request for quotation
CAS: 193149-74-5
Tel: 86-10-10086 81625225
Email: yeqim@boyechemicals.com
Mobile: 86-15387090420
QQ: 1377812380 Click to send a QQ message
Wechat: 15387090420
Zouping Mingxing Chemical Co., Ltd.
Product Name: 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt Request for quotation
CAS: 193149-74-5
Tel: 0086-13605431940
Email: xuli678323@126.com
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193149-74-5
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