Name | Acetylcholine chloride |
Synonyms | ACH acecholin ACECOLINE Acetylcholine chloride ACETYLCHOLINE CHLORIDE ACETYLCHOLINI CHLORIDUM acetylcholinehydrochloride 2-(Acetyloxy)-N,N,N-trimethylethanium chloride (2-Hydroxyethyl)trimethylammoniumchlorideacetate 2-(acetyloxy)-n,n,n-trimethyl-ethanaminiuchloride |
CAS | 60-31-1 |
EINECS | 200-468-8 |
InChI | InChI=1/C7H16NO2.ClH/c1-7(9)10-6-5-8(2,3)4;/h5-6H2,1-4H3;1H/q+1;/p-1 |
Molecular Formula | C7H16ClNO2 |
Molar Mass | 181.66 |
Density | 1.1235 (rough estimate) |
Melting Point | 146-150 °C (lit.) |
Water Solubility | Soluble IN COLD WATER |
Solubility | H2O: 100 mg/mL. Soluble in water, ethanol and propylene glycol, soluble in chloroform and acetic acid, insoluble in ether. |
Appearance | White crystalline powder |
Color | White |
Merck | 14,87 |
BRN | 3571875 |
Storage Condition | room temp |
Stability | Stable. Substances to be avoided include strong oxidizing agents. Protect from moisture - very hygroscopic. |
Sensitive | Hygroscopic |
Refractive Index | 1.5560 (estimate) |
MDL | MFCD00011698 |
Physical and Chemical Properties | White crystalline powder, easy to moisture absorption, ammonia odor, very salty. Mp149-152. The pH value of 10% aqueous solution is about 5, which is easy to decompose in hot water and alkaline solution. This product is soluble in water, ethanol and propylene glycol, soluble in chloroform and acetic acid, insoluble in ether. Low toxicity, LD50 (rat, oral) 2500 mg/kg. |
Use | As a substrate for the determination of acetylcholinesterase |
In vitro study | Acetylcholine chloride (ACh chloride; 10 μM) opens the calcium channel and the fluorescence value and intracellular free calcium will increase significantly when the medium is with high calcium, while these will decrease when the medium is without calcium in sweat gland epithelial cells. |
Hazard Symbols | Xi - Irritant |
Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S37/39 - Wear suitable gloves and eye/face protection S36 - Wear suitable protective clothing. |
WGK Germany | 2 |
RTECS | FZ9800000 |
FLUKA BRAND F CODES | 3-8-10-21 |
TSCA | Yes |
HS Code | 29379000 |
Hazard Class | IRRITANT |
Toxicity | LD50 in mice, rats (mg/kg): 20, 22 i.v., 170, 250 s.c., 3000, 2500 i.p. (Molitor) |
Reference Show more | 1. Zhou Xuechun, Li Yueyue, Yan Guiming. Effect of paeonol on airway hyperresponsiveness in asthmatic rats [J]. Chinese herbal medicine 2017 040(003):703-706. 2. Effects of aerobic exercise on colonic myenteric plexus and colonic dysfunction in type Ⅱ diabetic rats [J]. Chinese Journal of pathophysiology, 2013, 29(6):1059-1064. 3. Pei Kun, Xia Jian Gao, Chen Hai-Fang, et al. Mechanism of naringin on isolated tracheal smooth muscle of guinea pigs [J]. New Traditional Chinese Medicine & Clinical Pharmacology, 2015, 26(001):17-21. 4. Ming Haixia, Chen Yanwen, Huang Shizuo, et al. Effects of Gansu honeysuckle on bile secretion and gallbladder smooth muscle in experimental animals [J]. Journal of Traditional Chinese Medicine, 2014, 42(1):65-67. 5. Wu Shanshan, He Xi Cheng, Yin Hai, Hu Lin, Li Wei, Hu Chang Jiang. Study on the pharmacological effects of petroleum ether fraction "urine contraction" in suoquan pill [J]. Pharmacology and Clinic of traditional Chinese medicine, 2018,34(05):15-18. 6. Liu Junxiu, Zhu Kelan, Ma Delu. Effects of dioscin on isolated rat aortic rings [J]. Journal of Tianjin Medical University, 2012, 26 (01):24-26. 7. Wenke, Jiao Jianjie, Liu Yinzhong, et al. Effects of Huoxiang Zhengqi dropping pills on gastrointestinal function in normal and acetylcholine-stimulated animals [J]. Chinese Journal of new drugs, 2011, 16 (07):76-80. 8. Wei Ying, Pei Kun, Chen Haifang, et al. Effect and mechanism of synephrine on isolated tracheal smooth muscle of guinea pig [J]. Chinese Journal of Experimental prescriptions, 2015, 21(1):158-162. 9. Shi Na, Wang Zhuqing, Wang Haizhen, Wang Jing, Zhou Pingping, Jiang Yuxin, Wang Haihua. Comparison of high-salt diet-induced and two-kidney-one-clip-induced hypertensive rat models [J]. Journal of Wannan Medical College, 2016,35(05):418-422. 10. Shao Feng, Chen Huijuan, Liu Ronghua, etc. Effects of total alkaloids from heishunpian on the motility of isolated small intestinal smooth muscle of Diarrhea and rats induced by Senna [J]. Chinese herbal medicines, 2013, 36(011):1805-1809. 11. Wang Xichun, Zhu Xiaoyan, Zhang Yafei, etc. Effects of deoxynivalenol exposure on neurotransmitters, lipid peroxidation and calcium homeostasis in hippocampal neurons of piglets [J]. Chinese Journal of Animal Nutrition, 2018, 30(09):402-409. 12. Xu Haiyun, Wang Shuai Shuai, Tan Chengjie, Hu Jufang, Chen Xiaojun. Relaxant effect of ethanol extract of gelsemium on isolated intestinal muscles of rabbits and its mechanism [J]. Journal of Hunan Agricultural University (Natural Science Edition),2020,46(04):461-465. 13. Tang Xiaoqian, Liu, Yun, Wu Kechun, et al. Relaxant effect of alkaloids from Phyllanthus urinaria L. On isolated intestinal muscles of rabbits [J]. Chinese Journal of Veterinary Medicine 2019. 14. Gu Yue, Ju AI Chun, Man Shu Li, etc. Safety Evaluation of Yiqi Fumai for injection [J]. Drug evaluation studies, 2018. 15. Hui Heping, Jin Hui, Yang Xiaoyan, Li Xiu Zhuang, Xin Aiqin Bo. Chemical structure and morphology analysis of Lanzhou lily polysaccharide BHP-1 [J]. Chinese Journal of Experimental prescriptions, 2020, 26(08):170-175. 16. [IF = 3.361] Linjia Sun et al."Neuroprotectative effect of total glycosides from Paeonia against neurotoxicity induced by strychnos alkaloids related to recovering levels of neurotransmitters and neuroendocrine hormones in rat serum and brain."Rsc Adv. 2018 Aug;8(5 17. [IF=6.057] Ghazala Ashraf et al."Extension of duplex specific nuclease sensing application with RNA aptamer."Talanta. 2022 May;242:123314 |
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
biological activity | compound Acetylcholine Chloride is a neurotransmitter, acts on the peripheral nervous system (PNS) and central nervous system (CNS) of many organisms, including humans. |
Target | Value |
Animal Model: | Male and female out-bred albino mice weighing 18-22 g (sepsis) |
Dosage: | 20 mg/kg |
Administration: | SC; single dose |
Result: | Significantly reduces mortality of mice from sepsis induced by intraperitoneal injection of 2×10 9 E. coli bacterial bodies and the blood levels of proinflammatory cytokines TNF-α, IL-1β, and IL-6. |
Use | is used as a substrate for the determination of acetylcholinesterase; Clinically as a cholinergic drug. |
production method | with chloroethanol and trimethylamine aqueous solution as the starting material reaction, and then acetic anhydride acylation, finally refined. |
category | toxic substances |
toxicity grade | poisoning |
Acute toxicity | oral-rat LD50: 2500 mg/kg; Oral-mouse LD50: 3000 mg/kg |
flammability hazard characteristics | flammability; Toxic nitrogen oxides and chloride fumes released by thermal decomposition |
storage and transportation characteristics | warehouse ventilation and low temperature drying |
extinguishing agent | dry powder, foam, sand, carbon dioxide, water mist |