| biological activity | DJ001 is a highly specific, selective and non-competitive protein tyrosine phosphatase-Sigma (ptpσ) inhibitor, IC50 1.43 μm. DJ001 had no inhibitory activity against other phosphatases and only moderate inhibitory activity against protein phosphatase 5. DJ001 promotes hematopoietic stem cell regeneration. |
| Target | IC50: 1.43 μm |
|
Cell Line:
| BM KSL cells |
|
Concentration:
| 5 ng/mL, 10 ng/mL, 100 ng/mL, 1000 ng/mL |
|
Incubation Time:
| 3 days or 7 days |
|
Result:
| Increased the percentages and numbers of BM KSL cells. Also significantly increased the numbers of colony forming cells (CFCs) in 3 day culture of BM KSL cells.
Significantly decreased the percentage of apoptotic BM KSL cells in C57BL/6 mice at 24 h following 500 cGy TBI. |
|
Animal Model:
| Female C57BL/6 mice (8-10-week-old) irradiated with total body irradiation (TBI) |
|
Dosage:
| 5 mg/kg |
|
Administration:
| Subcutaneous injection; for 24 hours |
