27072-45-3
Fluorescein isothiocyanate
CAS: 27072-45-3
Molecular Formula: C21H11NO5S
27072-45-3 - Names and Identifiers
| Name | Fluorescein isothiocyanate |
| Synonyms | FITC Fitc 5(6)-FITC Fluorescein5(6)-isot Isothiocyanatofluorescein Fluorescein isothiocyanate 5-Lsothiocyanato Fluorescein Spectracomp(Tm) Fitc Particles 5(6)-Fluorescein Isothiocyanate 5-Fluorescein Isothiocyanateisomer Fluorescein-5-Isothiocyanate Isomer I 5-Fluorescein Isothiocyanate, Isomer I Fluorescein 5-Isothiocyanate, Isomer 1 Fluorescein Isothiocyanate (Mixture of 5- and 6- isoMers) Fluorescein 5(6)-isothiocyanate,FITC, Fluorescein isothiocyanate 2-(6-Hydroxy-3-Oxo-3H-Xanthen-9-Yl)-5-Isothiocyanato-Benzoic Acid 3',6'-Dihydroxy-5-Isothiocyanato-3H-Spiro[2-Benzofuran-1,9'-Xanthen]-3-One Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-dihydroxy-5(or 6)-isothiocyanato- 3',6'-Dihydroxy-5(Or 6)-Isothiocyanatospiro[Isobenzofuran-1(3H),9'-[9H]Xanthen]-3-One |
| CAS | 27072-45-3 |
| EINECS | 248-207-7 |
| InChI | InChI=1/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H |
| InChIKey | WBSWSUYBEIMVMI-UHFFFAOYSA-N |
27072-45-3 - Physico-chemical Properties
| Molecular Formula | C21H11NO5S |
| Molar Mass | 389.38 |
| Density | 1.54g/cm3 |
| Melting Point | >360°C(lit.) |
| Boling Point | 708.6°C at 760 mmHg |
| Flash Point | 382.4°C |
| Solubility | DMSO (Slightly), Methanol (Slightly) |
| Vapor Presure | 9.53E-21mmHg at 25°C |
| Appearance | powder |
| Color | Brown to Dark Brown |
| Merck | 14,4089 |
| BRN | 1407295 |
| Storage Condition | -20°C |
| Stability | Moisture Sensitive |
| Sensitive | `sensitive` to light, humidity and heat |
| Refractive Index | 1.753 |
| MDL | MFCD00005063 |
| Physical and Chemical Properties | Yellow powder, soluble in water, decomposition in water. Soluble in alkaline aqueous solution, and a strong row of green fluorescence disappeared, and precipitation, after adding alkali and dissolved and fluorescence. Insoluble in acetone, ether and petroleum ether. Hygroscopic. |
| Use | This product is a biochemical reagent, but also a medical diagnostic drug, mainly used for fluorescent dyes in fluorescent antibody technology, can be combined with various antibody proteins, the combined antibody does not lose the specificity of binding with certain antigens, and has a strong green fluorescence in alkaline solution. Used in medicine, agronomy and animal husbandry, etc., can be caused by bacteria Virus and parasites and other diseases for rapid diagnosis. |
27072-45-3 - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | R42/43 - May cause sensitization by inhalation and skin contact. R36/37/38 - Irritating to eyes, respiratory system and skin. R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. R42 - May cause sensitization by inhalation |
| Safety Description | S22 - Do not breathe dust. S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S24/25 - Avoid contact with skin and eyes. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37 - Wear suitable protective clothing and gloves. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10 |
| HS Code | 29321900 |
27072-45-3 - Reference
| Reference Show more | 1. Li Shuai, ailenzhong, Zhang Hui, et al. Effect of passion fruit pectin and its octenyl succinate esterification on the stability of acidified skim milk beverage [J]. Food and Fermentation Industry, 2020, v.46;No.407(11):155-160. 2. Tao Li, Xiaoliang Zhao, NEI, Ying-Lan et al. Study on brain permeability of sinomenine and tetramethylpyrazine in CQ analgesic compound [J]. Journal of Pharmacy 2019 v.54(12):186-193. 3. Jin-Rong Wang, Xiao-Na Guo, Jun-Jie Xing, Ke-Xue Zhu,Revealing the effect mechanism of NaCl on the rheologic properties of daugh of Chinese traditional hand-stretched drived drinks, Food Chemistry, Volume, 2032020, 126606, ISSN 0308-8146, https://doi.o 4. Bo Fan, Yang Xing, Ying Zheng, Chuan Sun & Guixian Liang (2016) pH-responsive thiolated chitosan nanoparticles for oral low-molecular weight heparin delivery: in vitro and in vivo evaluation, Drug Delivery, 23:1, 238-247, DOI: 10.3109/10717544.2014.909908 5. Fan, Hui-Jie, et al. "Effects and mechanism of action of Huang-Lian-Jie-Du-Tang in atopic dermatitis-like skin dysfunction in vivo and in vitro." Journal of ethnopharmacology 240 (2019): 111937.https://doi.org/10.1016/j.jep.2019.111937 6. [IF=5.076] Huichao Xie et al."An Available Strategy for Nasal Brain Transport of Nanocomposite Based on PAMAM Dendrimers via In Situ Gel."Nanomaterials-Basel. 2019 Feb;9(2):147 7. [IF=3.591] Yuan Zhang et al."Folate-targeting and bovine serum albumin-gated mesoporous silica nanoparticles as a redox-responsive carrier for epirubicin release."New J Chem. 2019 Feb;43(6):2694-2701 8. [IF=3.534] Wenfeng Ni et al."Dual-Targeting Nanoparticles: Codelivery of Curcumin and 5-Fluorouracil for Synergistic Treatment of Hepatocarcinoma."J Pharm Sci-Us. 2019 Mar;108:1284 9. [IF=9.147] Yan Tian et al."Effects of protein concentration, pH and NaCl concentration on the physicochemical, interfacial, and emulsifying properties of β-conglycinin."Food Hydrocolloid. 2021 Sep;118:106784 10. [IF=7.514] Yi Cai et al."Molecularly imprinted microspheres based multiplexed fluorescence method for simultaneous detection of benzimidazoles and pyrethroids in meat samples."Food Chem. 2020 Jul;319:126539 11. [IF=7.514] Jin-Rong Wang et al."Revealing the effect mechanism of NaCl on the rheological properties of dough of Chinese traditional hand-stretched dried noodles."Food Chem. 2020 Aug;320:126606 12. [IF=7.053] Yan Tian et al."The role of conformational state of pH-shifted β-conglycinin on the oil/water interfacial properties and emulsifying capacities."Food Hydrocolloid. 2020 Nov;108:105990 13. [IF=6.953] Dong Yu-Hao et al."Study on the pharmacokinetics of mulberry fruit polysaccharides through fluorescence labeling."Int J Biol Macromol. 2021 Sep;186:462 14. [IF=6.707] Haili Zhao et al."Spatial modulation of biomolecules immobilization by fabrication of hierarchically structured PEG-derived brush micropatterns: An versatile cellular microarray platform."Appl Surf Sci. 2020 Nov;529:147056 15. [IF=6.331] Yangyang Huang et al."Platelet-derived nanomotor coated balloon for atherosclerosis combination therapy."J Mater Chem B. 2020 Jul;8(26):5765-5775 16. [IF=4.952] Feng Xue et al."Prevention of frozen-dough from deterioration with incorporation of glutenin-polyphenols conjugates prepared by ultrasound."Lwt Food Sci Technol. 2021 Nov;151:112141 17. [IF=4.939] Yixin Dong et al."ERK-Peptide-Inhibitor-Modified Ferritin Enhanced the Therapeutic Effects of Paclitaxel in Cancer Cells and Spheroids."Mol Pharmaceut. 2021;18(9):3365-3377 18. [IF=4.821] Ge Wang et al."Directional evolution of TetR protein and development of a fluoroimmunoassay for screening of tetracyclines in egg."Microchem J. 2019 Nov;150:104184 19. [IF=3.267] Jiaxin Liu et al."Cyclic RGD Peptide Targeting Coated Nano Drug Co-Delivery System for Therapeutic Use in Age-Related Macular Degeneration Disease."Molecules. 2020 Jan;25(21):4897 20. [IF=2.464] Bing Jie Jia et al."Synthesis of molecularly imprinted microspheres and development of a fluorescence method for detection of chloramphenicol in meat."Luminescence. 2021 Nov;36(7):1767-1774 21. [IF=5.279] Tong He et al.Development of a Dihydropteroate Synthase-Based Fluorescence Polarization Assay for Detection of Sulfonamides and Studying Its Recognition Mechanism.J Agr Food Chem. 2021;69(46):13953-13963 22. [IF=6.953] Jiaqi Tan et al."Separation of gallic acid from Cornus officinalis and its interactions with corn starch."Int J Biol Macromol. 2022 May;208:390 23. [IF=5.89] Yuanyuan Jin et al."Preparation and Evaluation of Liposomes and Niosomes Containing Total Ginsenosides for Anti-Photoaging Therapy."Frontiers in Bioengineering and Biotechnology. 2022; 10: 874827 24. [IF=3.167] Hongkun Xue et al."Separation of principal component dihydromyricetin from Ampelopsis grossedentata by high-speed counter-current chromatography and its interaction with corn starch."JOURNAL OF FOOD SCIENCE 25. [IF=4.616] Fanfan Yu et al."Gold Nanocluster-Based Ratiometric Fluorescent Probe for Biosensing of Hg2 ions in Living Organisms."ANALYST. 2022 Apr ;: |
27072-45-3 - Introduction
It's hygroscopic. It can bind to various antibody proteins, and the bound antibody does not lose the specificity of binding to a certain antigen, and there is still strong green fluorescence in the alkaline solution. After adding acid, it precipitates and precipitates, the fluorescence disappears, and it is slightly soluble in acetone, ether and petroleum ether. Insoluble in water. Biochemical research. Fluorescent antibody tracing. Rapid diagnosis of diseases caused by viruses and parasites.
Last Update：2022-10-16 17:24:10
27072-45-3 - Reference Information
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| biological activity | 5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is a fluorescent dye of amine active derivative. It has a wide range of applications and is used as antibody and other probe labels. Fluorescence microscopy, flow cytometry, immunofluorescence methods such as ELISA and Western blotting experiments are used. |
| Use | This product is a biochemical reagent and a medical diagnostic drug. It is mainly used as a fluorescent dye in fluorescent antibody technology. It can bind to various antibody proteins. The antibody does not lose the specificity of binding to a certain antigen, and has strong green fluorescence in alkaline solution. It is used in medicine, agronomy and animal husbandry, and can quickly diagnose diseases caused by local bacteria, viruses and parasites. Protein fluorescent labeling reagent is also a medical diagnostic drug. It is mainly used as a fluorescent dye in fluorescent antibody technology. It can bind to various antibody proteins. After binding, the antibody does not lose the specificity of binding to a certain antigen, and has strong green fluorescence in alkaline solution. It is used in medicine, agronomy and animal husbandry, and can quickly diagnose diseases caused by local bacteria, viruses and parasites. |
| production method | 4-nitrophthalic anhydride (see C8H3NO5,[5466-84-2]) and resorcinol react at 195-200 ℃ for 10h, cyclically form a mixture of 3 '-nitro fluorescent yellow and 4'-nitro fluorescent yellow, then acetylated with acetic anhydride and hydrolyzed with saturated sodium hydroxide-ethanol solution to obtain 4 '-nitrofluorescein. Then 4 '-nitrofluorescein is dissolved in ammonia water, rested with nickel and hydrazine hydrate for 2h at room temperature, and reduced to 4'-amino fluorescein. The most elevated acylation, elimination to obtain fluorescein isothiocyanate. |
Last Update：2024-04-10 22:29:15
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Product Name: Fluorescein5(6)-isothiocyanate Visit Supplier Webpage Request for quotationCAS: 27072-45-3
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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