29270-56-2
4-Fluoro-7-nitrobenzofurazan
CAS: 29270-56-2
Molecular Formula: C6H2FN3O3
29270-56-2 - Names and Identifiers
| Name | 4-Fluoro-7-nitrobenzofurazan |
| Synonyms | 4-Fluoro-7-nitrobenzofurazan 7-Nitro-4-fluoro-2,1,3-benzoxadiazole 7-Fluoro-4-nitro-2,1,3-benzoxadiazole 4-Fluoro-7-nitrobenzofurazan((D)NBD-F) 4-fluoro-7-nitro-2,1,3-benzooxadiazole 4-fluoro-7-nitrobenzo[c][1,2,5]oxadiazole 4-Fluoro-7-nitrobenzofurazan4-Fluoro-7-nitro-2,1,3-benzoxadiazole |
| CAS | 29270-56-2 |
| InChI | InChI=1/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H |
29270-56-2 - Physico-chemical Properties
| Molecular Formula | C6H2FN3O3 |
| Molar Mass | 183.1 |
| Density | 1.6753 (estimate) |
| Melting Point | 52-54°C(lit.) |
| Boling Point | 308.6±45.0 °C(Predicted) |
| Flash Point | >230°F |
| Solubility | It is soluble in DMSO, DMF, CH3OH, CH3CN and CHCl3. |
| Vapor Presure | 0.00123mmHg at 25°C |
| Appearance | Brown crystalline bulk |
| Color | Brown |
| BRN | 1077571 |
| pKa | -5.59±0.50(Predicted) |
| Storage Condition | Inert atmosphere,Store in freezer, under -20°C |
| Sensitive | Sensitive to light |
| Refractive Index | 1.637 |
| MDL | MFCD00010196 |
| Use | It is often used for fluorescent labeling of amino acids and amine compounds in HPLC analysis. |
| In vitro study | As the percentage of the organic phase changes, the retention time of NBD-F remains relatively stable, while the retention times of the derivatization products changes. The pH of the mobile phase affects the separation of the NBD-F and the derivatization products. NBD-F is a fluorescent derivatization reagent that is originally developed for amino acid analysis, and recently applied to the analysis of other amino acid derivatives such as N-methyl-D-aspartic acid and glutathione. The use of NBD-F appears to have several advantages in that the derivatization procedure is simple and its derivatives are highly stable. |
29270-56-2 - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/38 - Irritating to eyes and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S39 - Wear eye / face protection. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-21 |
| HS Code | 29349990 |
29270-56-2 - Introduction
Used for labeling proteins, peptides and antibodies, soluble in DMF and DMSO
Last Update：2022-10-16 17:24:20
29270-56-2 - Reference Information
| Biological activity | NBD-F (4-Fluoro-7-nitrobenzofurazan) is a pre-fluorescent reagent for amino acid analysis. The NBD-F reacts with primary or secondary amines to produce fluorescent products, which are used for the analysis of amino acids and low molecular weight amines. |
Last Update：2024-04-09 19:05:11
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