In vitro study | VER-49009 inhibits the endogenous ATPase activity of the recombinant yeast Hsp90 protein, which has an IC50 of 167 nM, VER-49009 has antiproliferative activity against a subset of human tumor cell lines and non-tumor cells in vitro. VER-49009 induces the dissociation of HSP72 and HSP27 from their binding proteins (C-RAF,B-RAF, survivin, and PRMT5), which in turn leads to cell cycle arrest and apoptosis. VER-49009 inhibit cell proliferation and induce G2 arrest of hepatocyte CFSC, which gives a reasonable strategy to prevent liver fibrosis. |