3209-30-1
Toluidine bule
CAS: 3209-30-1
Molecular Formula: C28H20N2Na2O10S2
3209-30-1 - Names and Identifiers
| Name | Toluidine bule |
| Synonyms | Toluidine bule disodium 4,4'-[(9,10-dihydro-4,8-dihydroxy-9,10-dioxo-1,5-anthrylene)diimino]bis[toluene-3-sulphonate] 2,2'-[(9,10-Dihydro-4,8-dihydroxy-9,10-dioxo-1,5-anthracenediyl)diimino]bis(5-methylbenzenesulfonic acid sodium) salt |
| CAS | 3209-30-1 |
| EINECS | 221-718-2 |
3209-30-1 - Physico-chemical Properties
| Molecular Formula | C28H20N2Na2O10S2 |
| Molar Mass | 654.57534 |
| Use | Use 1. Reagents for redox titration in chemistry. 2. It can be used in ophthalmology in medicine to check corneal defects. 3. Used in histochemistry for the determination of deoxyribonucleic acid and ribonucleic acid. 4. Diagnosis of diphtheria. 5. Used in dye industry, such as wool and silk printing and dyeing. |
3209-30-1 - Uses and synthesis methods
overview
Topuidine Blue (TB) is a biochemical reagent widely used in many fields. For example, an indicator used for redox titration in chemistry, in phosphoric acid medium, ascorbic acid (hereinafter represented by HB) is used to reduce it to a reduced substance, which can be applied to the redox titration process of titration with a reducing agent; In addition, toluidine blue is used in ophthalmology in medicine to check corneal defects, diagnose diphtheria, and also in the dye industry.
Toluidine blue is a thiazide dye, which is a basic dye and can be used as a nuclear stain. This type of dye generally has 2 chromophore groups, one is oral amine group and the other is quinone benzene ring. Quinone benzene ring has obvious heterochromaticity, which can be expressed as α-heterochromaticity (negative), which is blue, that is positive; β-heterochromaticity (weakly positive), purple or purple; & gamma;-Heterochromaticity (positive), red. However, this kind of heterodyne staining turns into orthochromia when dehydrated by alcohol. This dye is characterized by good staining effect on Nissl body of neurons, and can also dye mucus, cartilage matrix, mast cells, etc.
synthetic route
760g(6.28mol)N,N-dimethylaniline and 1800mL hydrochloric acid (37%) were added into a 30L glass reactor. Cooling to 0 ℃, adding 3L aqueous solution containing 420g(6.10mol) of sodium nitrite dropwise. After dropping, stir at 0 ℃ for 20min. Add 2kg of sodium thiosulfate and 2.4kg of zinc chloride containing crystal water and stir at room temperature for 5 hours. Adjust the pH value with 350g of sodium hydroxide aqueous solution, add 640g of o-toluidine (5.98mol) dropwise, stir evenly, add 1.2kg of potassium dichromate aqueous solution 5L dropwise, and react for 2h. Add 1L concentrated sulfuric acid to the reaction mixture, heat to 70 ℃, separate the precipitate after cooling, extract toluidine blue from the precipitate with 80 ℃ hot water, and add sodium chloride to the extract for salting out.
clinical application
1. It can quickly dye mast cells;
2. In the gastric mucosal immunohistochemical reaction, after toluidine blue re-staining, the Helicobacter pylori in the gastric mucosal tissue was stained blue;
3. Asymptomatic oral squamous cell carcinoma in vivo can be identified early. Benign ulcers usually have very clear marginal staining, while the edges of precancerous or malignant lesions are diffuse;
4. Oral toluidine blue intragastric staining is helpful for the preliminary diagnosis of gastric cancer;
5. Toluidine blue can be used for cervical smear staining, which can stain epithelial nuclei. This method is simple and effective, but it is limited to the early diagnosis of cervical cancer.
Toluidine blue staining inspection method
1. rinse your mouth with clear water, spit clean, dip a cotton swab with 1% acetic acid and apply it to the lesion to dissolve the mucus attached to the surface.
2. dip a cotton swab with 1% toluidine blue solution and apply it to the lesion and surrounding mucosa, stay for at least 1min, and then rinse with clear water.
3. scrub the coated toluene amine salt with a cotton swab dipped in 1% acetic acid. if the dye is not washed off, it is positive.
Last Update：2024-04-10 22:29:15
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CAS: 3209-30-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
CAS: 3209-30-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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