4-NITROFLUORESCEIN

Name	5-Nitro-3,6-Dihydroxyspiro[Isobenzofuran-1(3H),9-(9H)Xanthen]-3-One
Synonyms	4-NITROFLUORESCEIN 4-NITROFLUOROESCEIN 3',6'-dihydroxy-6-nitrospiro[2-benzofuran-3,9'-xanthene]-1-one 3',6'-dihydroxy-6-nitro-spiro[2-benzofuran-3,9'-xanthene]-1-one 3',6'-dihydroxy-6-nitro-spiro[isobenzofuran-3,9'-xanthene]-1-one 5-Nitro-3,6-Dihydroxyspiro[Isobenzofuran-1(3H),9-(9H)Xanthen]-3-One 3',6'-Dihydroxy-5-nitrospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one 5-NITRO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE 5-Nitro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one
CAS	3326-35-0

Molecular Formula	C20H11NO7
Molar Mass	377.3
Density	1.72±0.1 g/cm3(Predicted)
Melting Point	>300°C
Boling Point	692.0±55.0 °C(Predicted)
Solubility	almost transparency in NaOH100g/L
Appearance	powder to crystal
Color	Light yellow to Brown
pKa	9.29±0.20(Predicted)
Storage Condition	2-8°C
Stability	Light and Moisture Sensitive
MDL	MFCD00135108

EPA chemical substance information	information provided by: ofmpeb.epa.gov (external link)
Application	4-nitrofluorescein can be used as a pharmaceutical synthesis intermediate.
preparation	the preparation of 4-nitrofluorescein is as follows: 8.6g(18.7mmol) 4-nitrofluorotriethyl acetate was dissolved in 87ml of 10% sodium hydroxide solution and 432ml of methanol and heated gently. The precipitate was filtered off and dissolved in 500ml of water. The solution was acidified with hydrochloric acid and left to stand overnight in the dark. The crystals were filtered off and washed with water. 6.9g(18.2mmol),97% red crystals of 4-nitrofluorescein were obtained. C20H11NO7; Molecular weight: 377.30;1HNMR(300MHz,DMSO-d6)δ10.20(brs,2H,OH),8.65(s,1H,nsi-4H),8.57(d,J = 8.4,1H,nsi-6H),7.57(d,J = 8.3,1H,nsi-7H),6.70-6.64(m,4H,xa-4,5,2,7h),6.56(d,J = 8.7,2H,xa-1,8h). ESIMS:376.1[M-H ](100).

Last Update：2024-04-10 22:29:15