4-NPP,Na2
1.10-Phenanthroline
CAS: 1485-17-2
Molecular Formula: C12H8N2
4-NPP,Na2 - Names and Identifiers
4-NPP,Na2 - Physico-chemical Properties
| Molecular Formula | C12H8N2 |
| Molar Mass | 180.20532 |
4-NPP,Na2 - References
| Overview | Biochemical Reagent refers to biological materials or organic compounds related to life science research, as well as clinical diagnosis, reagents for medical research. Due to the wide range of life sciences, rapid development, so the variety of reagents, complex nature. Under the background of the rapid development of biotechnology in the 21st century, biochemical reagents will be developed on a large scale for scientific research and medical application. |
| Introduction | biochemical reagents classification:(1) immune reagents including antibodies and antisera, normal serum and complement, antigen, immunohistochemistry reagents, cell culture reagents, cell separation reagents, gel diffusion and Electrophoresis Reagents. (2) reagents for genetic engineering include gene expression and Gene recombination, synthetic proteins, hormones, Nucleic Acid Synthesis Reagents, nucleic acid preparations, endonucleases, etc. (3) mutagens and carcinogens are mainly used to determine the carcinogenicity of toxic substances in the workplace and living environment and the mutagenicity of chemical poisons. (4) clinical diagnostic reagents are mainly used for a large class of chemical reagents used in diagnostic tests such as clinical pathological diagnosis, biochemical diagnosis, liquid crystal diagnosis, isotope diagnosis and general chemical diagnosis in the medical system. (5) industrial chemicals including trial-made development of industrial chemicals, more than four thousand kinds, is still increasing. [picture] Biochemical reagent physical picture. |
| detection index | with the continuous development of inspection technology, the hospital laboratory basically carries out new projects every year, due to the large number of kit manufacturers, how to select the kit has become a difficult problem in the laboratory. If the performance evaluation of the diagnostic kit is evaluated in terms of sensitivity, precision, repeatability, accuracy, specificity, linearity, reportable range, anti-interference test, traceability or method comparison results, that is time-consuming and laborious. Automatic biochemical analyzer has the characteristics of trace, rapid, multiple, accurate and good repeatability. With the wide application of automatic biochemical analyzer, the problem of cross contamination in the determination process is also concerned. When detecting the content of total bile acid in the specimen, it is still found that the single item of bile acid is particularly high after adjusting the project Channel, and the result is normal when reexamining. Bile acid in liver and gallbladder disease, neonatal jaundice, intrahepatic cholestasis of pregnant women, coronary heart disease and other aspects are important detection indicators. The reagent loading mechanism of the multi-channel biochemical analyzer requires contact with various reagent components, so when the reagent needle, stirring needle and fixed cuvette are not completely cleaned or the efficiency of the cleaning solution is low, residual reagents or reaction products contaminate the reagent needle, agitating the needle, and fixing the cuvette, which may lead to instability of the results of adjacent test items. |
| detection error | The data show that there are three main reasons for the instability of bile acid results: first, the reagent component contains a certain contaminated item; Second, the reagent contains the substrate to be measured in the next test or a certain reagent component contained in the reagent, which has a role in the substrate to be measured in the next reaction, therefore, it directly affects the result of the next reaction measurement; Third, the reaction induced by the reagent brings indirect interference to the reaction process of the next project. From the above experimental data, we found that the abnormal high results of bile acid were caused by carrying pollution in the whole process from the screening of pollution items to the elimination of interference, and the positive interference of uric acid on bile acid was the greatest, total cholesterol and triacylglycerol took second place. From the reagent composition, total cholesterol and triacylglycerol reagents contain sodium cholate, 4-chlorophenol: uric acid, total cholesterol and triacylglycerol all contain peroxidase, 4-AAP. From the reaction principle, total cholesterol and triacylglycerol are hydrogen peroxide and 4-aminoantipyrine and phenol in The peroxidase catalyzed formation of benzoquinimine; uric acid is hydrogen peroxide and 4 amino antipyrine and DHBS also catalyzed by peroxidase to produce colored compounds. While other reagents in the composition and reaction process did not appear peroxidase enzyme and sodium cholate. And sodium cholate can promote lipoprotein esterase, cholesterol esterase and other corresponding substrate binding, promote the hydrolysis of esterase, so it is speculated that sodium cholate may have the reaction characteristics of bile acid, high concentrations of sodium cholate may cause cross-contamination between reagents and thus interfere with the determination of total bile acids; And peroxidase may enhance the total bile acid cycling enzyme reaction. |
| Cross-contamination | the causes of cross-contamination between reagents are as follows: one reagent contains a component of another reagent; A component of one reagent as an intermediate in the reaction of another reagent, participating in the reaction; the reaction product produced by one reagent and the sample is similar to the product produced by the reaction of another reagent and the sample; The pH of one reagent, this has a large effect on the pH of the other reagent and therefore on the reaction. The cross contamination between reagents has a great impact on the accuracy of the test results, so it is necessary to discharge the cross contamination. In the automatic biochemical analyzer, generally in the arrangement of the project requires at least one non-interference between the two projects, and the most thorough approach is to be interfered with the project before the project. It can be seen that we set up a booster water rinse program is enough to eliminate the interference of uric acid, but can not remove the interference of total sterols and triglycerides, thus, after the determination of the total cholesterol and triacylglycerol items, the procedure of flushing the fixed cuvette with a special cleaning solution was set up. After the setting, the interference of the total cholesterol and triacylglycerol was removed. Due to the complexity of the reagent composition, the uniqueness of the real-time instrument detection, the possible cross contamination between reagents is not the same, so if the accuracy and precision of the results are high, we should not only be familiar with the operation of the instrument and carefully read the reagent instructions to set the appropriate parameters, but also in the usual instrument for careful maintenance and maintenance. |
| references | [1] Yu Shu Wu, Wang Xia. Analysis of the influence of Biochemical reagent pollution on the detection results of automatic biochemical analyzer [J]. International Journal of Laboratory Medicine, 2013,(24):3403-3404. [2] Zhang Meixiang, Wang Jianping, Wang Jingwen, Zhang Yihong, Kong Li. Comparison and Evaluation of three retinol-binding protein biochemical reagents [J]. Journal of Laboratory Medicine and clinic, 2013,(07):782-783. [3] Huang Qianting, Jia Xiaolin, LV Weibiao, Tang Yuehua. Cross-contamination of 23 commonly used biochemical reagents [J]. Journal of Laboratory Medicine and clinic, 2009,(19):1612-1613 1615. [4] Deng Zhenghui, Zhang Hua, Li Longping. Cross-contamination of biochemical reagents for bile acid determination and preventive measures [J]. China Practical Medicine, 2008,(10):55-56. |
Last Update：2024-04-09 21:04:16
