5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN
CAS: 150347-59-4
Molecular Formula: C29H19O11
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN - Names and Identifiers
| Name | 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN |
| Synonyms | CFSE 5-CARBOXYFLUORESCEIN-NHS DIACETATE CARBOXYFLUORESCEIN DIACETATE, NHS ESTER 5-CARBOXY-DI-O-ACETYLFLUORESCEIN N-SUCCINIMIDYL ESTER 5(6)-CARBOXYFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER 5(6)-Carboxyfluorescein diacetate, succinimidyl ester(CFDA) 5(6)-CARBOXYFLUORESCEIN DIACETATE-N-HYDROXY-SUCCINIMIDE ESTER 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN |
| CAS | 150347-59-4 |
| InChIKey | QSWYNOQSGMUDQZ-UHFFFAOYSA-N |
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN - Physico-chemical Properties
| Molecular Formula | C29H19O11 |
| Molar Mass | 557.461 |
| Melting Point | 152-154 °C(lit.) |
| Boling Point | 765.4℃ at 760 mmHg |
| Solubility | DMSO, DMF, Acetonitrile, THF and Chloroform. |
| Appearance | Solid |
| pKa | 6.45(at 25℃) |
| Storage Condition | -20°C |
| Sensitive | Sensitive to light and humidity |
| MDL | MFCD00037466 |
| Physical and Chemical Properties | Bioactive Carboxyfluorescein succinimidyl ester (CFSE, 5(6)-Carboxyfluorescein diacetate succinimidyl ester, CFDA-SE, 5(6)-CFDA N-succinmidyl ester) is a fluorescent cell dye. CFSE has cell permeability. Through its succinimide group, it is covalently bound to intracellular lysine residues and other amine sources. |
| Use | A Non-fluorescent cell permeant amine-reactive probe for long term tracing of cell. |
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN - Risk and Safety
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 8-10-21 |
| HS Code | 32129000 |
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN - Nature
pH indicator color change ph range
Weak green 'uorescence (6.0) to strong green' uorescence (7.2)
maximum wavelength (& lambda;max)
<300nm
main applications
Diagnosis of cancer, restricting HIV replication, antiinflammatory agent, detecting bacteria, cell proliferation assays
Last Update：2024-04-10 22:29:15
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN - Uses and synthesis methods
in vitro studies
The binding of CFDA-SE fluorescent dye to cells is very stable. It has little toxic effect on cells and does not affect the cell viability of labeled cells. CFDA-SE is a suitable marker for cell labeling in cell experiments. After labeling, it can be maintained in cells for 24 hours, which is suitable for long-term observation of labeled cells.
In vivo studies
In vivo experiments, it is feasible to label cells with CFSE, and there is almost no toxicity to labeled cells and neighboring cells. It is proved by T cell recovery of peripheral lymphoid organs that CFSE has certain advantages in relatively long-term migration experiments.
Last Update：2024-04-10 22:29:15
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