CFDA-SE MedChemExpress (MCE)

Product Name	5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN
Synonyms	CFSE 5-CARBOXYFLUORESCEIN-NHS DIACETATE CARBOXYFLUORESCEIN DIACETATE, NHS ESTER 5-CARBOXY-DI-O-ACETYLFLUORESCEIN N-SUCCINIMIDYL ESTER 5(6)-CARBOXYFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER 5(6)-Carboxyfluorescein diacetate, succinimidyl ester(CFDA) Synonyms CFSE 5-CARBOXYFLUORESCEIN-NHS DIACETATE CARBOXYFLUORESCEIN DIACETATE, NHS ESTER 5-CARBOXY-DI-O-ACETYLFLUORESCEIN N-SUCCINIMIDYL ESTER 5(6)-CARBOXYFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER 5(6)-Carboxyfluorescein diacetate, succinimidyl ester(CFDA) 5(6)-CARBOXYFLUORESCEIN DIACETATE-N-HYDROXY-SUCCINIMIDE ESTER 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN
CAS	150347-59-4
EINECS
Chemical Formula	C29H19O11
Molecular Weight	557.461
inchi
Package	1 mg;1 mg;5 mg;5 mg;10 mg;10 mg;25 mg;25 mg;50 mg;50 mg
Price	Email to quote
Descriptions	CFDA-SE CFDA-SE MedChemExpress (MCE) HY-D0938 150347-59-4 CFSE Descriptions CFDA-SE CFDA-SE MedChemExpress (MCE) HY-D0938 150347-59-4 CFSE 5(6)-Carboxyfluorescein diacetate succinimidyl ester 5(6)-CFDA N-succinmidyl ester 99.01% -20°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months -20°C, 1 month (sealed storage, away from moisture and light) Room temperature in continental US may vary elsewhere. CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells. Preparation of CFDA-SE working solution1.1 Preparation of the stock solutionDissolve 1 mg of CFDA-SE in 0.1794 mL of DMSO to obtain 10 mM of CFSE.Note: It is recommended to store the stock solution at -20 ℃ or -80 ℃ away from light and avoid repetitive freeze-thaw cycles.1.2 Preparation of CFDA-SE working solutionDilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of CFDA-SE working solution.Note: Please adjust the concentration of CFDA-SE working solution according to the actual situation.Cell staining2.1 For suspension cells: Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.2.2 Add 1 mL of CFDA-SE working solution, and then incubate at room temperature for 30 minutes.2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.2.4 Wash twice with PBS, 5 minutes each time.2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer. Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2]. 515 485 \| \| \| \| \| \| \| \| \| \| [1]. Banks HT, et al. Quantifying CFSE Label Decay in Flow Cytometry Data. Appl Math Lett. 2013 May 1 26(5):571-577. [Content Brief]* [2]. A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013 Chapter 9:Unit9.11. [Content Brief] [3]. Cui J, et al. Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization. J Cell Mol Med. 2021 Jun 25(12):5586-5601. [Content Brief] [4]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017 6(7): e1333210. [Content Brief]
Last Update	2025-04-01 14:45:47

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