5-Ph-IAA
5-Ph-IAA
CAS: 168649-23-8
Molecular Formula: C16H13NO2
5-Ph-IAA - Names and Identifiers
| Name | 5-Ph-IAA |
| Synonyms | 5-Ph-IAA 5-(2-phenyl)-indole-3-acetic acid 1H-Indole-3-acetic acid, 5-phenyl- |
| CAS | 168649-23-8 |
5-Ph-IAA - Physico-chemical Properties
| Molecular Formula | C16H13NO2 |
| Molar Mass | 251.28 |
| Density | 1.291±0.06 g/cm3(Predicted) |
| Boling Point | 521.3±35.0 °C(Predicted) |
| Solubility | DMSO : 80 mg/mL |
| pKa | 4.47±0.30(Predicted) |
| Storage Condition | -20℃ |
| Use | 5-Ph-IAA is a derivative of IAA. 5-Ph-IAA, a ligand, established the auxin-induced degron 2 system (AID2) with the OsTIR1 (F74G) mutant. AID2 induces rapid and efficient consumption of mAID fusion proteins to study protein function in living cells. AID2 can inhibit tumors. |
| In vitro study | HCT116 cells constitutively expressing OsTIR1(F74G) are exposed to 5-Ph-IAA (1 µM; 6 hours), leading to DHC1-mAC degradation, which means the AID2 system better than the original AID system in generating conditional mutant cell lines. Western Blot Analysis Cell Line: HCT116 cells Concentration: 1 µM Incubation Time: 6 hours Result: Depleted DHC1-mAC efficiently in HCT116 cells constitutively expressing OsTIR1(F74G). |
| In vivo study | 5-Ph-IAA (0-10 mg/kg; intraperitoneally injection; every day; 7 days), used for the AID2 system ,is sufficient to deplete mAID-BRD4 and TOP2A-mAC, leading to tumour suppression. Animal Model: Balb/c-nu female mice (7 weeks old; 16–20 g ) bearing mAID-BRD4 and TOP2A-mAC xenograft tumours cells Dosage: 0 mg/kg, 1 mg/kg, 3 mg/kg and 10 mg/kg Administration: Intraperitoneally injection; every day; 7 days Result: Displayed significant tumour suppression of mAID-BRD4 xenografts and TOP2A-mAC xenografts. |
5-Ph-IAA - Reference
| Reference Show more | 1. Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 2020 Nov 11;11(1):5701. |
5-Ph-IAA - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 3.98 ml | 19.898 ml | 39.796 ml |
| 5 mM | 0.796 ml | 3.98 ml | 7.959 ml |
| 10 mM | 0.398 ml | 1.99 ml | 3.98 ml |
| 5 mM | 0.08 ml | 0.398 ml | 0.796 ml |
Last Update：2024-01-02 23:10:35
5-Ph-IAA - Reference Information
| biological activity | 5-Ph-IAA is a derivative of IAA. 5-Ph-IAA, a ligand, together with OsTIR1 (F74G) mutants established the auxin-induced degron 2 system (AID2). AID2 induces rapid and efficient consumption of mAID fusion proteins to study protein function in living cells. AID2 can inhibit tumors. |
| in vitro study | HCT116 cells constitutively expressing OsTIR1(F74G) are exposed to 5-Ph-IAA (1 µM; 6 hours), leading to DHC1-mAC degradation, which means the AID2 system better than the original AID system in generating conditional mutt cell lines. Western Blot Analysis Cell Line: HCT116 cells Concentration: 1 µM Incubation Time: 6 hours Result: Depleted DHC1-mAC efficiently in HCT116 cells constitutively expressing OsTIR1(F74G). |
| Cell Line: | HCT116 cells |
| Concentration: | 1 µM |
| Incubation Time: | 6 hours |
| Result: | Depleted DHC1-mAC efficiently in HCT116 cells constitutively expressing OsTIR1(F74G). Displayed significant tumour suppression of mAID-BRD4 xenografts and TOP2A-mAC xenografts. |
| in vivo study | 5-ph-IAA (0-10 mg/kg; intraperitoneally injection; every day; 7 days), used for the AID2 system ,is sufficient to deplete mAID-BRD4 and TOP2A-mAC, leading to tumour suppression. Animal Model: Balb/c-nu female mice (7 weeks old; 16-20 g ) bearing mAID-BRD4 and TOP2A-mAC xenograft tumours cells Dosage: 0 mg/kg, 1 mg/kg, 3 mg/kg and 10 mg/kg Administration: Intraperitoneally injection; every day; 7 days Result: Displayed significant tumour suppression of mAID-BRD4 xenografts and TOP2A-mAC xenografts. |
| Animal Model: | Balb/c-nu female mice (7 weeks old; 16–20 g ) bearing mAID-BRD4 and TOP2A-mAC xenograft tumours cells |
| Dosage: | 0 mg/kg, 1 mg/kg, 3 mg/kg and 10 mg/kg |
| Administration: | Intraperitoneally injection; every day; 7 days |
Last Update：2024-04-10 22:41:30
