55779-48-1
Coelenterazine
CAS: 55779-48-1
Molecular Formula: C26H21N3O3
55779-48-1 - Names and Identifiers
55779-48-1 - Physico-chemical Properties
| Molecular Formula | C26H21N3O3 |
| Molar Mass | 423.46 |
| Density | 1.32±0.1 g/cm3(Predicted) |
| Melting Point | 176–181 ℃ (decompose) |
| Boling Point | 641.4±65.0 °C(Predicted) |
| Solubility | methanol and ethanol: soluble |
| Appearance | solid |
| Color | yellow |
| Maximum wavelength(λmax) | ['429nm'] |
| pKa | 9.91±0.15(Predicted) |
| Storage Condition | -20°C |
| Sensitive | Moisture & Light Sensitive |
| MDL | MFCD00467176 |
| Use | Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine are used as bioluminescent donors for detecting protein-protein interactions in bioluminescence resonance energy transfer (BRET). Coelenterazine is a superoxide anion sensitive chemiluminescence probe, which is also used for chemiluminescence detection of peroxynitrite. |
| In vitro study | Detection of various biological processes has shown that coelenterazine (CTZ) can be catalyzed by luciferase, such as the Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Luc), it will emit blue light. In Gli36 cells expressing Gli36-Gluc or Gli36-Rluc, coelenterazine (8 μm/ml) exhibited a bioluminescent signal. In RIN-5F,4T1,HCT 116,HEK 293,293FT,HeLa and INS-1 cells, coelenterazine can be used to determine superoxide anion concentration. |
| In vivo study | In mice implanted with 4T1, Coelenterazine was able to detect cancer-associated superoxide anions and was used to evaluate the role of superoxide anions in cancer cell biology, as well as their role in pathology. |
55779-48-1 - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10 |
| HS Code | 29339900 |
55779-48-1 - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 2.361 ml | 11.807 ml | 23.615 ml |
| 5 mM | 0.472 ml | 2.361 ml | 4.723 ml |
| 10 mM | 0.236 ml | 1.181 ml | 2.361 ml |
| 5 mM | 0.047 ml | 0.236 ml | 0.472 ml |
Last Update：2024-01-02 23:10:35
55779-48-1 - Reference Information
| Biological applications | calcium indicator; detecting luciferase,protease,gene expression, nucleacids, proteins,stem cells,quantum dot projections, superoxide; tracing protein dynamics; as a substrate for luciferase,alka |
| biological activity | Coelenterazine is a luminescent enzyme substrate used to monitor receptor genes in BRET,ELISA and HTS techniques. |
Last Update：2024-04-09 15:16:56
