Name | 6-carboxyfluorescein |
Synonyms | 6-FAM, 6-CF Carboxyfluorescein 6-carboxyfluorescein 6-Carboxyfluorescein Hydrate 6-Carboxyfluorescein (6-FAM) 6-Carboxyfluorescein,6-FAM, FLUOS 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid 3',6'-dihydroxy-1-oxo-spiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid 3',6'-dihydroxy-1-keto-spiro[isobenzofuran-3,9'-xanthene]-5-carboxylic acid 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-6-carboxylic acid 3-Oxo-3',6'-dihydroxy-1,3-dihydrospiro[isobenzofuran-1,9'-[9H]xanthene]-6-carboxylic acid |
CAS | 3301-79-9 |
EINECS | 636-503-2 |
InChI | InChI=1/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25) |
Molecular Formula | C21H12O7 |
Molar Mass | 376.32 |
Density | 1.73±0.1 g/cm3(Predicted) |
Melting Point | >300°C(lit.) |
Boling Point | 736.4±60.0 °C(Predicted) |
Flash Point | 271°C |
Solubility | DMSO: soluble |
Vapor Presure | 9.09E-23mmHg at 25°C |
Appearance | Solid |
Color | Yellow to orange |
Maximum wavelength(λmax) | ['492nm'] |
BRN | 54341 |
pKa | 3.3, 4.6, 6.4, 7.0(at 25℃) |
Storage Condition | Sealed in dry,2-8°C |
Sensitive | Sensitive to light |
Refractive Index | 1.816 |
MDL | MFCD00036873 |
Use | fluorescent dye |
Hazard Symbols | Xi - Irritant |
Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37 - Wear suitable protective clothing and gloves. |
WGK Germany | 3 |
FLUKA BRAND F CODES | 10-21 |
HS Code | 3204 90 00 |
pH indicator color change ph range | Weak green 'uorescence (6.0) to strong green 'uourescence (7.2) |
main applications | Diagnosis of hematologic cancer, nongastric diseases, detection of genetically modified wheat, chromosomes, gene expression, nucleic acid, hepatitis A virus, avian influenza virus subtype H5 and H5N1, SARS virus, herpex simplex virus |
biological field application | Diagnosing bladder cancer; evaluating corneal endothelial barrier function; As a substrate for measuring protein kinase activity, methyltransferase activity, phospholipase activity, rhodanese activity,sulfotransferase activity;use in ophthalmology |
EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
Biological activity | 6-FAM (6-Carboxyfluorescein) is an isomer of carboxyfluorescein, mainly used for nucleic acid sequencing and labeling. |