Effect | Coffee bean alcohol and coffee alcohol (K-C) have potential effects on carcinogen-activated hepatocyte pigment P450 enzyme (CYP450) and sulfur transferase (S ULT). |
The application of | K-C has an inhibitory effect on CYP450 rather than a complete inhibitory effect. K-C inhibition of CYP450 and S ULT may weaken the activation of carcinogens and play a role in chemical cancer prevention. The complex mixture and its purified monomer components caffein alcohol and caffeanol will show significantly different effects in terms of activity. |
biological activity | Kahweol is one of the components of coffee Coffea Arabica and has anti-inflammatory, anti-angiogenic and anti-cancer activities. Kahweol adipogenesis of lipogenesis inhibited by activation of kinase (AMPK) and increased glucose uptake. Kahweol induces apoptosis (apoptosis). |
target | AMPK; adipogenesis; apoptosis |
in vitro study | Kahweol (20-40μM; 24 hours) decreases TGF-β-induced CTGF protein expression in AML12 and LX2 cells. Kahweol (20-40μM; 1 hour) decreases TGF-β-induced phospho-Smad2/3 expression in AML12 cells, but does not affect expression in LX2 cells. Kahweol (20-40μM) significantly decreased TGF-β-stimulated phospho-ERK and JNK expression in primary hepatocytes only. kahweol (0-25μ g/ml; 6-24 hours) increased the phosphorylation of AMPK and its downstream target Acetyl-CoA carboxylase (ACC) in a time-dependent in 3T3-L1 cells. kahweol (0-25μ g/ml) significantly affects protein expression of PPARγ, C/EBPα, FABP4, and FASN, that regulate adipocyte differentiation and lipid metabolism. Western Blot Analysis Cell Line: 3T3-L1 cells Concentration: 0,5, 10, 25 μg/ml Incubation Time: Result: Reduced PPARγ, C/EBPα, FABP4, and FASN expression in a dose-dependent manner. |
Cell Line:
| 3T3-L1 cells |
Concentration:
| 0, 5, 10, 25 μg/ml |
Incubation Time:
| |
Result:
| Reduced PPARγ, C/EBPα, FABP4, and FASN expression in a dose-dependent manner. |