Name | Methyl Green, zinc chloride salt |
Synonyms | CI 42590 CI NO 42590 LIGHT GREEN B DOUBLE GREEN SF METHYL GREEN, ZINC CHLORIDE ETHYL GREEN ZINC CHLORIDE SALT METHYL GREEN, ZINC CHLORIDE SALT Methyl Green, zinc chloride salt |
CAS | 7114-03-6 |
EINECS | 230-415-4 |
InChI | InChI=1/C27H35N3.4ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4 |
Molecular Formula | C27H35BrCl3N3Zn |
Molar Mass | 653.23 |
Melting Point | >300°C(lit.) |
Water Solubility | 70 g/L (20 ºC) |
Solubility | Soluble in water, ethanol; insoluble in xylene |
Appearance | Solid |
Color | Green |
Maximum wavelength(λmax) | ['629 nm, 423 nm'] |
Storage Condition | Store at RT. |
Stability | Stable. Incompatible with strong oxidizing agents. |
Sensitive | Sensitive to light |
MDL | MFCD00151094 |
Physical and Chemical Properties | Methyl green is mainly used for dyeing fabrics and biological dyeing, and exists in two forms. Formula I: molecular formula C26H33Cl2N3. Molecular weight 458.49. Green Crystal, with golden yellow or light green powder. Soluble in water, ethanol-soluble, insoluble in ether. It is usually a double salt of zinc chloride. Formula II: molecular formula c27h35brcln3. Molecular weight 516.98. Green powder, soluble in water is green blue. The solution was yellow at pH 0.2 and blue at pH 1.8. preparation method: Methyl green product is obtained by reacting bromoethane and violet. |
Use | Used for bacterial staining (fresh specimen nucleus staining) Methyl green-hydrochloric acid mixture is used to detect sperm, gonococcus and mast cell staining. |
Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R50/53 - Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment. R34 - Causes burns R22 - Harmful if swallowed |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S61 - Avoid release to the environment. Refer to special instructions / safety data sheets. S60 - This material and its container must be disposed of as hazardous waste. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. |
WGK Germany | 3 |
HS Code | 29215900 |
Reference Show more | 1. Yang Chenghuan, Yang Yao, Li Kuo, et al. Effects of red kidney bean phytohemagglutinin on placental tissue of middle and late pregnancy mice [J]. Chinese Journal of Family Planning, 2015, 23(8). 2. [IF = 8.742] Xinwen Gong et al."Self-assembled hierarchical MXene/COF membranes for efficient dye membranes." JOURNAL OF mem brane SCIENCE. 2022 Sep;657:120667 |
pH range of acid-base indicator discoloration | Yellow (0.1) to greenish-blue (2.3) |
Biological applications | |
methyl green-panning reaction | the phosphate groups of DNA and RNA make both easy to bind to basic dyes. Both methyl green and peyronin are basic dyes, but under suitable conditions, methyl green has a specific affinity for high Poly DNA, and peyronin can stain RNA, but the specificity is poor. Therefore, treating tissue sections with a mixture of Methyl green and peyronin at a pH of about 4.8 (the effect is better with Karot's or sinker's fixative) can cause DNA and RNA to be stained green and red, respectively, the exact mechanism is not clear, only the binding ability of Methyl green to DNA is related to the integrity of the double helix space configuration of DNA molecule. For example, RNA is extracted with 1% NaCl or ribonuclease, and then methyl green-panning reaction is carried out, and as a result, the presence of RNA in the cell can be confirmed. |
artificial dyes | that is aniline dyes or coal tar dyes, many kinds, a wide range of applications. Its disadvantage is that it is easy to fade by sunlight, aniline blue, bright green, methyl green and so on. In the production of attention to grasp the pH, and to avoid direct sunlight, but also after several years does not fade. methyl green is a basic dye. It is green powder, soluble in water (solubility 8%) and alcohol (solubility 3%). Methyl green is the most valuable cell and stain, cytology is commonly used to stain the cytoplasm, with acid fuchsin can be used for plant xylem staining. |
Use | for bacterial staining (fresh specimen nuclei staining) methyl green-hydrochloric acid mixture for sperm, neisseria gonorrhoeae and mast cell staining. nuclear stain, bacterial stain (fresh specimen, nuclear stain). The methyl green-hydrochloric acid mixture was used to assay sperm, chromatin staining, Neisseria gonorrhoeae and mast cell staining. |