81295-42-3

81295-42-3 - Names and Identifiers

Name	XbaI
Synonyms	XbaI XBA I e.c.3.1.23.41 XBA I RESTRICTION ENZYME endodeoxyribonucleasexbai restrictionendonucleasexbai restrictionendodeoxyribo-nucleasxbai Nuclease, restriction endodeoxyribo-, XbaI restriction endonuclease xba I from*xanthomonas B
CAS	81295-42-3
EINECS	279-739-8

81295-42-3 - Physico-chemical Properties

Molecular Formula	C97H123N38O58P9
Molar Mass	3028.01
Appearance	buffered aqueous glycerol solution
Storage Condition	−20°C

81295-42-3 - Risk and Safety

WGK Germany	1
RTECS	RF0758900

81295-42-3 - Introduction

XbaI (X after BamHI, A means Aerogenes) is an endonuclease that belongs to the family of restriction endonucleases. It is produced by a type of bacteria called Xanthomonas bacteria.

The enzymatic activity of XbaI depends on the temperature in the presence of magnesium ions, and the optimum temperature is 37°C. It recognizes a specific DNA sequence, the 5 '-TCTAGA-3', and divides the DNA into two pieces for two positions of that sequence. The cleaved DNA ends typically result in sticky ends that have single-stranded DNA overhangs, making the sticky ends easy to attach to other DNA molecules.

XbaI has a wide range of applications in molecular biology research. It is commonly used in DNA sequencing, subcloning, plasmid construction and other experiments. Due to its specific cleavage sequence, it can be used for the identification and localization of specific DNA fragments.

The preparation of XbaI is usually through genetic engineering methods, the corresponding DNA sequence in the Xanthomonas bacteria genome is put into a suitable expression vector, and the expression and purification are carried out by host cells such as E. coli.

In safe operation, pay attention to follow the laboratory's biosafety procedures when using XbaI. XbaI is an enzyme that is not directly toxic, but still requires careful handling and proper storage. Any enzyme manipulation should avoid direct contact with the skin and eyes, and avoid inhalation or ingestion. Appropriate personal protective equipment, such as laboratory gloves and glasses, should be worn. In addition, follow proper waste disposal procedures to ensure safe disposal of enzymes.

Last Update：2024-04-09 21:04:16