856925-71-8
(S)-3a-Hydroxy-6-methyl-1-phenyl-3,3a-dihydro-1H-pyrrolo[2,3-b]quinolin-4(2H)-one
CAS: 856925-71-8
Molecular Formula: C18H16N2O2
856925-71-8 - Names and Identifiers
856925-71-8 - Physico-chemical Properties
| Molecular Formula | C18H16N2O2 |
| Molar Mass | 292.33 |
| Density | 1.31±0.1 g/cm3(Predicted) |
| Melting Point | 210-212°C |
| Boling Point | 507.3±60.0 °C(Predicted) |
| Solubility | DMSO: soluble5mg/mL |
| Appearance | Yellow solid |
| Color | Light Yellow to Yellow |
| pKa | 11.15±0.20(Predicted) |
| Storage Condition | -20°C |
| MDL | MFCD08460907 |
| Use | (−)-Blebbistatin |
| In vitro study | Blebbistatin inhibits cell division, alters the smooth movement of fish keratocytes, and inhibits the spontaneous blistering of a filamin-deficient cell line. Blebbistatin inhibited the enzymatic activity of non-muscle myosin IIA, non-muscle myosin IIB, and the HMM fragment of rabbit skeletal muscle myosin S1, but not smooth muscle globin. Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity and also inhibits the in vitro activity of non-muscle myosins IIA and IIB, whereas it inhibits very weakly smooth muscle myosin (IC50=80 μm). Blebbistatin effectively inhibits Dictyostelium myosin II, but extremely weakly inhibits Acanthamoeba myosin II. Blebbistatin does not inhibit representative I,V, and X-type myosin superfamily members. Blebbistatin does not competitively bind with nucleotides to skeletal muscle myosin subfragment -1. Blebbistatin preferentially binds to ATPases, interacts with ADP and phosphate at the active site, slowing phosphorus release. Blebbistatin interfered neither with myosin binding to actin nor with ATP-induced actin dissociation. |
| In vivo study | Blebbistatin dose-dependently and completely relax both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz. |
856925-71-8 - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. R36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37 - Wear suitable protective clothing and gloves. |
| WGK Germany | 3 |
856925-71-8 - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 3.421 ml | 17.104 ml | 34.208 ml |
| 5 mM | 0.684 ml | 3.421 ml | 6.842 ml |
| 10 mM | 0.342 ml | 1.71 ml | 3.421 ml |
| 5 mM | 0.068 ml | 0.342 ml | 0.684 ml |
Last Update：2024-01-02 23:10:35
856925-71-8 - Reference Information
| biological activity | (-)-Blebbistatin ((S)-(-)-Blebbistatin) is a kind of cell permeability inhibitor, acting on non-myosin IIATPase, IC50 is ~ 2 μm in cell-free test, does not inhibit myosin light chain kinase (MLCK), inhibits the contraction of the cleavage groove, does not interfere with the assembly of mitotic or contractile loops. |
| Target | Value |
| non-muscle myosin II ATPases (Cell-free assay) | 0.5 μM-5 μM |
| purpose | Blebbistatin blocked myosin II-dependent cell processes. It blocks. Blebbistatin also rapidly disrupted directed cell migration and cytolysis in vertebrate cell . Blebbistatin blocks. [Blebbistatin:a]D = -103 (c = 1.21 CHCl3) (-)-1-Phenyl-1,2,3,4-tetrahydro-4-hydroxypyrrolo[2,3-b]-7-methylquinolin-4-one |
Last Update：2024-04-09 02:00:10
Supplier List
Spot supply
Product Name: (-)-Blebbistatin Visit Supplier Webpage Request for quotationCAS: 856925-71-8
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
Spot supply
Product Name: (-)-Blebbistatin Visit Supplier Webpage Request for quotationCAS: 856925-71-8
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
View History