9001-15-4
Creatine phosphokinase
CAS: 9001-15-4
Molecular Formula:
9001-15-4 - Names and Identifiers
| Name | Creatine phosphokinase |
| Synonyms | CK CPK AMP phosphotransferase Creatine phosphokinase |
| CAS | 9001-15-4 |
| EINECS | 232-585-5 |
9001-15-4 - Physico-chemical Properties
| Solubility | 0.25 M glycyl-glycine, pH 7.4: soluble5.0mg/mL, clear, colorless to slightly yellow |
| Appearance | salt-free, lyophilized powder |
| Color | white |
| Storage Condition | -20°C |
| MDL | MFCD00130876 |
| Physical and Chemical Properties | White salt-free lyophilized powder, soluble in water, the optimum pH value of 6.0-7.0 (positive reaction), 9.0 (reverse reaction). Stability: The pH value is less than 5 under the condition of inactivation, thawing and freezing also makes the enzyme inactivation;-20 ℃,pH value is 6.6, in 0.1mot/L imidazole buffer can be stable for 4 weeks, neutral 60% ethanol solution is the most stable, dilute solution to add 0.1% albumin or β-mercaptoethanol can increase the stability; Freeze-dried products at -20 ℃ can be stored for 12 months, stored at 4 deg C for 6 months 15% loss of vitality. Inhibitors of mercury chloride benzoic acid, chelating agents, iodoacetic acid, triphosphate, adenosine, nitrite, sulfate, bromide, sulfite, chloride, fluoride thyroxine, 3, 5-dinitro-cresol, coenzyme I(NAD) L-triiodoformylamine (T3), malonate, iodine (I-), iodoacetic acid, orthophosphoric acid, pyrophosphoric acid, tripolyphosphoric acid, etc. Activators are Mg2 +, Mn2 +, 2-mercaptoethanol, dithiothreitol, glutathione, thiol N-acetylcysteine, etc. The absorption coefficient of the 1% aqueous solution at 280nm was 8.76. Enzyme reaction: creatine + adenosine triphosphate adenosine diphosphate + creatine phosphate. |
9001-15-4 - Risk and Safety
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-21 |
| HS Code | 35079090 |
9001-15-4 - Reference
| Reference Show more | 1. Yang, Qing, et al. "Ginsenoside compound K regulates amyloid β via the Nrf2/Keap1 signaling pathway in mice with scopolamine hydrobromide-induced memory impairments." Journal of Molecular Neuroscience 67.1 (2019): 62-71.https://doi.org/10.1007/s12031-018-1 2. Chen, Xijun, et al. "Ginsenoside compound K ameliorates Alzheimer's disease in HT22 cells by adjusting energy metabolism ." Molecular biology reports 46.5 (2019): 5323-5332.https:// doi.org/10.1007/s11033-019-04988-0 Note: For some products, we can only provide some information. We do not guarantee the authority of the information provided. uses: a class of enzymes that catalyze the conversion of adenosine diphosphate (ADP) and phosphocreatine to ATP and creatine. storage conditions: -20 °c solubility: 0.25 M glycyl-glycine, pH 7.4: soluble5.0 mg/mL, clear, colorless to clear yellow EC 2.7.3.2 |
9001-15-4 - Trait
White or off-white scales.
Last Update：2023-08-16 21:32:38
9001-15-4 - Definition of enzyme activity
One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 ℃
Last Update：2023-08-16 21:32:38
9001-15-4 - Reference Information
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| Effect | Creatine creatine kinase is also called creatine kinase. CPK is an organ-specific enzyme, which mainly exists in skeletal muscle, myocardium and brain tissue. CPK mainly catalyzes the biochemical reaction of creatine phosphate (CP) and adenosine diphosphate (ADP) in muscle to synthesize creatine (C) and adenosine triphosphate (ATP). |
| fixation method | it uses chitosan as raw material to prepare cross-linked chitosan solution first, then cross-linked chitosan microspheres, then graft lysine cross-linked chitosan resin is prepared, and finally creatine kinase is immobilized with graft lysine cross-linked chitosan resin as carrier. |
| use | used for biochemical research, food preservation, clinically commonly used as diagnostic enzymes, determination of phosphoric acid and phosphocreatine. Common diagnostic enzymes, determination of creatine and creatine phosphate. |
| production method | rabbit muscle is used as raw material, homogenized and heat treated by ethanol for the first time, denatured protein is removed, ethanol for the second time after magnesium sulfate extraction, and the product is recrystallized. |
Last Update：2024-04-09 21:04:16
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