9001-99-4
ribonuclease A from bovine pancreas
CAS: 9001-99-4
Molecular Formula:
9001-99-4 - Names and Identifiers
9001-99-4 - Physico-chemical Properties
| Melting Point | 243 °C (dec.) |
| Specific Rotation(α) | D25 (per mg N) -0.47° (c = 5) |
| Water Solubility | Soluble in water. |
| Solubility | Easily soluble in water. |
| Appearance | White powder |
| Color | white |
| PH | pH : 8.0 |
| Storage Condition | -20°C |
| Sensitive | Moisture Sensitive |
| MDL | MFCD00132181 |
| Physical and Chemical Properties | White crystal or freeze-dried powder, soluble in water, pI7.8, optimum pH 7.7. Stability: The most stable pH range is 2-4.5. Refrigerated freeze-dried powder or frozen enzyme solution can be kept unchanged for several years. Inhibitors include deoxyribonucleic acid (competitive inhibitor), denatured deoxyribonucleic acid (more effective than natural), 0.0005 mol/L Mg2. Enzymatic reaction: specifically catalyzes the cleavage of the ribose moiety 3 'and 5' phosphodiester bonds of ribonucleic acid to form oligonucleotides with 2 ',3'-cyclic phosphate derivatives. |
| Use | 1. Remove unhybridized RNA regions from DNA:RNA hybrids; 2. Determine the location of single base mutations in RNA or DNA. In RNA:DNA or RNA:RNA hybrids, if there is a single base fault, it can be recognized and cut by RNAse A. By gel electrophoresis analysis of the size of the cut product, the mismatch can be determined; 3, RNA detection. RNA enzyme protection analysis is a hybridization technology developed in recent years to detect RNA. 4. Degrade RNA molecules in DNA preparations. |
9001-99-4 - Risk and Safety
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| RTECS | RF0760000 |
| FLUKA BRAND F CODES | 10-21 |
| TSCA | Yes |
| HS Code | 35079090 |
9001-99-4 - Reference
| Reference Show more | 1. Zhou Yue Carnation, Wang yaner et al. Therapeutic effect of ginsenoside Rb1 on Kawasaki disease in mice [J]. Journal of Traditional Chinese Medicine 2017 v.45;No.236(06):40-44. 2. Zhang, Xiaoqiong, et al. "Metal-organic frameworks @ graphene hybrid aerogels for solid-phase extraction of non-steroidal anti-allergic drugs and selective enrichment of proteins." Analyst 141.13 (2016): 4219-4226.https:// doi.org/10.1039/C6AN00353B 3. Vyviurska, Olga, et al. "Comparison of commercial organic polymer‐based and silica‐based monolithic columns using mixtures of analytes differing in size and chemistry." Journal of separation science 41.7 (2018): 1558-1566.https://doi.org/10.1002/jssc.20170 4. [IF=5.833] Dai Qingzhou et al."Adsorption and specific recognition of DNA by using imprinted polymer layers grafted onto ionic liquid functionalized magnetic microspheres."Microchim Acta. 2017 Nov;184(11):4433-4441 5. [IF=5.118] Shan-Shan Zhang et al."Two Novel Multi-Functional Peptides from Meat and Visceral Mass of Marine Snail Neptunea arthritica cumingii and Their Activities In Vitro and In Vivo."Mar Drugs. 2018 Dec;16(12):473 6. [IF=4.616] Xiaoqiong Zhang et al."Metal-organic frameworks@graphene hybrid aerogels for solid-phase extraction of non-steroidal anti-inflammatory drugs and selective enrichment of proteins."Analyst. 2016 Jun;141(13):4219-4226 7. [IF=3.645] Olga Vyviurska et al."Comparison of commercial organic polymer-based and silica-based monolithic columns using mixtures of analytes differing in size and chemistry."J Sep Sci. 2018 Apr;41(7):1558-1566 8. [IF=3.205] Dandan Lan et al."Preparation of a hydroxyethyl-based monolithic column and its application in the isolation of intact proteins from complex bio-samples."J Chromatogr B. 2019 Jan;1104:89 9. [IF=2.696] He Zhang et al."Transdermal permeation effect of collagen hydrolysates of deer sinew on mouse skin, ex vitro, and antioxidant activity, increased type I collagen secretion of percutaneous proteins in NIH/3T3 cells."J Cosmet Dermatol-Us. 2020 Feb;19(2):519 |
9001-99-4 - Nature
Open Data Verified Data
This product is white crystal or lyophilized powder. Soluble in water. Isoelectric point pH a 8.0. Molecular weight 13000. The optimum pH was 7.0~8.0. The most stable in pH 2~4.5. The optimum temperature for the decomposition of yeast RNA was 65, and the activity was inhibited by heavy metals. The crystalline powder (salt-free) is stable at 0-4 °c for several years. It can catalyze the degradation of ribonucleic acid, can change the host cell metabolism, inhibit Virus synthesis, in vitro can inhibit the proliferation of influenza Virus, in chicken embryo can inhibit the formation of vaccinia, herpes Virus. Russian scholars have proved that this product is effective for acute pancreatitis.
Last Update：2024-01-02 23:10:35
9001-99-4 - Preparation Method
Open Data Verified Data
extraction from the fermentation broth of erythromycin producing bacteria.
Last Update：2022-01-01 11:11:01
9001-99-4 - Introduction
Ribonuclease (RNAse) is derived from bovine pancreas and is an endo-ribonuclease. Ribonuclease can change the metabolism of host cells, inhibit virus synthesis, inhibit the proliferation of influenza virus in vitro, and inhibit the formation of vaccinia and herpes virus in chicken embryos. Clinical ribonuclease is injected intramuscularly 180 mg per day, which is beneficial to the treatment of epidemic encephalitis. [Product Characteristics] 1. In the absence of cofactors and divalent cations, the effect of ribonuclease can be inhibited by placental RNA enzyme inhibitor or vanadium-ribonucleoside complex (vanadyl-ribonuclosidecomplex,VRC). 2. Enzymatic reaction: specifically catalyzes the cracking of the ribose part 3 'and 5' phosphodiester bonds of ribonucleic acid to form oligonucleotides with 2 ',3'-cyclic phosphate derivatives. [Stability] RNase has extremely wide reaction conditions and is extremely difficult to inactivate. The most stable pH range is 2-4.5, refrigerated freeze-dried powder or frozen enzyme liquid can be stored for several years without change in vitality. Inhibitors include deoxyribonucleic acid (competitive inhibitor), denatured deoxyribonucleic acid (more effective than natural) 0.0005mol/LMg2. [Definition of Unit] At 25 ℃ and PH5.0 (Kunitz), the decrease value of absorbance from A0 to Af is equivalent to the activity of one unit within 1 minute. A0 to Af is equivalent to the total conversion, Af is the final absorbance.
Last Update：2022-10-16 17:29:46
9001-99-4 - Use
Open Data Verified Data
- Anti-inflammatory enzyme preparation.
- usage and dosage of its medicinal ointment for the treatment of trauma and joint pain. Influenza and herpes were suppressed by Virus. And apply to acute pancreatitis; Daily intramuscular injection of 180mg, treatment of epidemic encephalitis.
Last Update：2022-01-01 11:11:01
9001-99-4 - Safety
Open Data Verified Data
sealed dry O ~ 4 deg C storage.
Last Update：2022-01-01 11:11:02
9001-99-4 - Reference Information
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Ribonuclease | Ribonuclease (RNase) is a class of nucleases that catalyze the degradation of RNA into small fragments. Ribonuclease can change the host cell metabolism, inhibit Virus synthesis, in vitro can inhibit influenza Virus proliferation, in chicken embryo can inhibit the formation of herpes Virus. RNase refers to an enzyme capable of hydrolyzing the phosphodiester bond of RNA, also known as ribonuclease (RNase). There are three common RNases, namely ribonuclease H, ribonuclease A and ribonuclease T1, which are hydrolases and can now be synthesized artificially. Ribonuclease H(RNase H) was first found in calf thymus tissue, and its coding gene has been cloned into E. Coli. Ribonuclease A(RNase A) is an endonuclease derived from bovine pancreas. Ribonuclease T1(RNase T1) is derived from Aspergillus oryzae. The reaction conditions of RNaseTl are very wide, and it is very difficult to be inactivated, and its catalytic activity is similar to that of RNase A. |
| Use | used in biochemical research, determination of nucleic acid structure, medicine for the treatment of trauma and joint pain, inhibition of influenza and herpes Virus, and apply to acute pancreatitis, epidemic encephalitis. Ribonuclease is an anti-inflammatory enzyme preparation. It can catalyze the degradation of ribonucleic acid, can change the host cell metabolism. Synthesis was inhibited by Virus. In vitro can inhibit the proliferation of influenza Virus. In chicken embryo bone can inhibit the formation of vaccinia, herpes Virus, and apply to acute pancreatitis. Its medicinal ointment for the treatment of local external trauma and joint pain. The patient was given 180mg intramuscular injection every day for epidemic encephalitis. Hermetically dried for preservation at 0-40C. • RNase protection assay • removal of unless specifically bound RNA • Analysis of RNA sequences • hydrolysis of RNA in protein samples • purification of DNA |
| production method | extraction of the fermentation broth of Americium produced from erythromycin. using bovine pancreas as raw material, the product was homogenized with dilute sulfuric acid, then fractionated and precipitated with ammonium sulfate, then recrystallized with ethanol, and then subjected to carboxymethylcellulose column chromatography to obtain ribonuclease A. |
Last Update：2024-04-09 20:52:54
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