9054-89-1
superoxide dismutase F. bovine erythro-cytes
CAS: 9054-89-1
Molecular Formula:
9054-89-1 - Names and Identifiers
9054-89-1 - Physico-chemical Properties
| Solubility | Dissolves readily at 5 mg/mL in 0.05 M potassium phosphate buffer, pH 7.8, containing 0.1 mM EDTA. |
| Appearance | powder |
| Color | blue-gray |
| Storage Condition | -20°C |
| MDL | MFCD00132404 |
| Physical and Chemical Properties | The main function of superoxide dismutase is to specifically remove the superoxide anion O-2 in the body, so as to relieve the damage to the body caused by the oxidation of the components in the body by the oxygen anion. Its half-life is short, usually only 6~10min. The relative molecular mass is large, it is not easy to penetrate the cell membrane, and it is easy to be inactivated by the action of protein-not-enzyme. Therefore, clinical application is limited. The enzyme is an acidic protein, which is relatively stable and heat-resistant. Ph7.6 ~ 9 stable, below ph6 and above 12h unstable. Especially extremely unstable below ph2. It has strong ability to resist pepsin and trypsin hydrolysis. There is no immune regulation and analgesic effect, and it does not affect the synthesis of prostaglandins and other inflammatory mediators. |
| Use | Superoxide dismutase from bovine erythrocytes has been used in a study to assess a kinetic model of radiation-induced inactivation of superoxide dismutase in nitrous oxide-saturated solutions. Superoxide dismutase from bovine erythrocytes has also be |
9054-89-1 - Risk and Safety
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S24/25 - Avoid contact with skin and eyes. S22 - Do not breathe dust. S23 - Do not breathe vapour. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-21 |
| HS Code | 35079090 |
9054-89-1 - Nature
Open Data Verified Data
- solid powder. This product is a peptide macromolecular metalloenzyme isolated from red blood cells, liver and other mammalian tissues, containing two subunits. According to its metal cofactor is divided into three types; Containing copper and zinc, the relative molecular mass of 32000, containing manganese relative molecular mass of 40000, containing iron relative molecular mass of about 40000. China's current products are mainly extracted from pig red blood cells in Cu-Zn-SOD. 0.32% Copper.
- The main function of superoxide dismutase is to specifically remove superoxide anion 02 in the body, thereby relieving damage to the body caused by oxidation of components in the body by the oxygen anion. Its half-life is short, usually only 6~10 min. Large molecular weight, not easy to penetrate the cell membrane, oral easily inactivated by proteolytic enzymes, so the clinical application is limited. The enzyme is an acidic protein that is relatively stable and heat-resistant. pH 7.6~9 stable, pH 6 and 12 above unstable, especially in the pH 2 below the very unstable. It has strong anti-pepsin and trypsin hydrolysis ability. There is no immune regulation and analgesic effect, and it does not affect the synthesis of inflammatory mediators such as prostaglandins.
Last Update：2024-01-02 23:10:35
9054-89-1 - Preparation Method
Open Data Verified Data
fresh porcine blood was collected and centrifuged to remove yellow plasma. The obtained red blood cells were washed with a sodium chloride solution by centrifugation, and the washing liquid was removed. This repeated washing 3 times, get clean red blood cells. Deionized water was added to clean red blood cells at 5 °c and stirred at 0. After 5H, 25ml of ethanol and 15ml of chloroform were added to each 10 ml of red blood cell solution, stirred for 15 min, centrifuged to remove hemoglobin, and the supernatant was collected. At 0 ° C., 1. 2 to 1.5 times the volume of acetone was added to the supernatant, and a large amount of precipitate was generated and centrifuged. The precipitate was dissolved in deionized water, centrifuged to remove insoluble proteins, and the supernatant was heat-treated at 55-65 °c for 10-15min, centrifuged to remove a large amount of heat-denatured proteins, and the yellow-green supernatant was collected. At 0 °c, an appropriate amount of acetone was added to the supernatant to precipitate and centrifuged. The precipitate was dissolved in deionized water, centrifuged to remove insoluble proteins, and the clear solution was dialyzed in a dialysis bag for 6-8 hours. The dialysate was added to the pH 7.6 phosphate buffer equilibrium DEAE-Sephadex A- 50 Column adsorption, and then pH 7.6 potassium phosphate buffer (2.5~50mmol/L) gradient elution, collecting eluate, ultrafiltration, concentration, freeze drying, that is, the superoxide dismutase product.
Last Update：2022-01-01 11:10:59
9054-89-1 - Introduction
Source: bovine blood molecular weight: 32000 ~ 34000D activity: ≥ 2500units/mg solid activity definition: one unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 ℃ in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial A550 of 0.025±0.005 per min
Last Update：2022-10-16 17:30:02
9054-89-1 - Use
Open Data Verified Data
- enzyme preparation. Treatment of systemic lupus erythematosus, rheumatoid arthritis, Dermatomyositis, scleroderma, autoimmune hemolytic anemia, thrombocytopenia and other autoimmune diseases; Treatment of myocardial ischemia and ischemia reperfusion syndrome; Can also be used for kidney, liver, heart and other organ protection and transplantation, limb replantation, plastic, beauty and other surgical processes; Treatment of certain cardiovascular diseases; For anti-aging. Preparation of superoxide dismutase for injection, each 4mg;8mg. Usage and dose intramuscular injection, 8mg once, 2-4 times per week; Intra-articular injection, once 4mg, once every 2 weeks; Radiation sequela, deep intramuscular injection, once 4mg, the radiotherapy was injected half an hour later.
- is used in the production of cosmetics and the like.
Last Update：2022-01-01 11:10:59
9054-89-1 - Safety
Open Data Verified Data
hepatitis, uremia, diabetes patients should not be used; Can not be combined with acidic or basic drugs, alcohol containing preparations, metal salts and antibiotics.
Last Update：2022-01-01 11:11:00
9054-89-1 - Reference Information
| Overview | SOD (superoxide dismutase) SOD is a series of enzymes, which is an active substance derived from living organisms, can eliminate the organism in the process of metabolism of harmful substances. Can effectively curb free radicals, free radicals capture, decomposition of free radicals, strong, targeted, is the only free radical as the substrate of the scavenging enzyme. |
| biochemical properties | superoxide dismutase can scavenge superoxide and protect cells from oxidative damage. The reaction of superoxide with non-radicals is spin-forbidden. In biological systems, this means that it primarily reacts with itself (disproportionation) or another biological free radical, such as nitric oxide. The superoxide anion radical (O2 −) can be disproportionated to O2 and hydrogen peroxide (H2O2) more rapidly (at pH = 7 with a reaction rate of ~ 105 M − 1 s − 1). However, superoxide can react with specific molecules (such as NO radicals) at a faster rate to generate peroxynitrite ions (O = N-O-O −), therefore, the catalytic effect of superoxide dismutase is particularly important. Moreover, the disproportionation reaction of superoxide is related to the square of its initial concentration, so although the half-life of superoxide at high concentrations is very short (e. G. 0.05 seconds at a concentration of mm), however, the half-life of superoxide at low concentrations is quite long (up to 14 hours at 0.1nm). |
| on the human body | superoxide dismutase has a strong anti-inflammatory effect, clinical for rheumatoid arthritis, osteoarthritis, radiation cystitis, can remove excess free radicals in the body, improve human immunity, delay aging; Anti-fatigue, regulate the female physiological cycle, delay menopause. |
| uses | superoxide dismutase preparation is mainly used for the treatment of systemic lupus erythematosus, dermatitis, scleroderma, rheumatoid arthritis, autoimmune hemolytic anemia, thrombocytopenia and other autoimmune diseases, treatment of myocardial ischemia and ischemia reperfusion syndrome. Can also be used for limb replantation, plastic, beauty and kidney, liver, heart and other organ protection and transplantation of surgical procedures. But diabetes, hepatitis, uremia patients should not use. Not with acidic or basic drugs, containing alcohol preparations, metal salts and antibiotics. Intramuscular injection, once 8mg, 2 to 4 times a week; Intra-articular injection, once 4mg, once every 2 weeks; Radiation sequelae, deep intramuscular injection, once 4mg, half an hour after radiotherapy. used in biochemical research, commonly used in clinical treatment of systemic lupus erythematosus, Dermatomyositis, rheumatoid arthritis, scleroderma, autoimmune hemolytic anemia, thrombocytopenia and other autoimmune diseases, the treatment of certain cardiovascular diseases, for anti-aging. This product is modified SOD lyophilized powder, more stable. Widely used in cosmetics, health food, scavenging free radicals, antioxidant, anti-aging. |
| production method | using bovine red blood cells as raw material, after washing, hemoglobin was separated and removed, and then column chromatography, the product was refined by dialysis. Fresh pig blood was collected and washed with milk, centrifuged to remove yellow plasma, and red blood cells were centrifuged with sodium chloride solution (9g/L, 0.9%) to remove the washing liquid, repeated washing for 3 times resulted in clean red blood cells. Fresh pig blood was centrifuged to remove plasma → red blood cells were collected and washed with NaCl for 3 times → red blood cells were washed and washed to remove hemolysis and hemoglobin. At a temperature of 50C, deionized water was added to clean red blood cells and stirred for 30min, then, 25ml of ethanol (95%) and 15ml of chloroform were added to each ml of red blood cell solution, stirred for 15min, centrifuged to remove hemoglobin, and the supernatant was collected. Clean red blood cells deionized water; 50;30min → hemolysis} hemolysis ethanol; Chloroform → dehemoglobin supernatant precipitation, heat treatment at about 00C, add 1.2~1.5 times the volume of acetone to the supernatant, after a large number of flocculent precipitates, The supernatant was removed by centrifugation to obtain a precipitate. The precipitate was dissolved by adding an appropriate amount of deionized water, and centrifuged to remove insoluble proteins. The supernatant was heat-treated at 55-650C for 10-15min, centrifuged to remove a large amount of heat-denatured proteins, and the yellow-green supernatant was collected. Supernatant acetone; 00 → precipitation precipitation deionized water; 600 → heat-treated yellow-green clear liquid precipitation, desolubilized protein, dialysis in 00C will add appropriate amount of acetone to the supernatant to produce a large number of flocculent precipitation, the supernatant was removed by centrifugation, and the precipitate was collected. The precipitate was dissolved by adding deionized water and stirring, and the insoluble protein was removed by centrifugation. The supernatant was placed in a dialysis bag for dynamic dialysis for 6-8H to obtain a dialysate. Yellow-green clear solution acetone; Deionized water; 00 → precipitate; Deinsoluble protein; Dialysate adsorption, elution, concentration, lyophilization the dialysate was added to phosphate buffer (2.5mmol/L) already used at pH 7.6 adsorption on A well-balanced DEAE-Sephadex A- 50 column, gradient elution with pH 7.6 potassium phosphate buffer (2.5~50mmol/L), collecting the eluate with superoxide dismutase activity, ultrafiltration, concentration, freeze-drying, namely superoxide dismutase finished dialysate DEAE-Sephadex A- 50; Potassium phosphate buffer; Ph7.6 → ^ adsorption, elution, ultrafiltration; Concentration, the conditions for the electrophoresis identification of the freeze-dried products were agarose gel (5g/L, 0.5g) plate electrophoresis, with pH 8.4 Tris-glycine as buffer, voltage gradient 22V/cm, current 3mA/cm, electrophoresis time 35min, fixed staining solution 5g/L(0.5%) amino black 10B, decolorization solution 7g/L(7%) acetic acid. |
Last Update：2024-04-09 20:52:54
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