| Name | Isomerase, glucose |
| Synonyms | Isomerase Isomerase, glucose immobilized glucose isomerast preparation glucose isomerase from actinoplanes missouriensis |
| CAS | 9055-00-9 |
| EINECS | 232-944-6 |
| Physical and Chemical Properties | An amorphous powder, granule, or liquid chemically nearly white to light brownish yellow or brown or pink. Soluble in water (particles are insoluble in water), insoluble in ethanol, chloroform and ether. The main enzyme is glucose (or xylose) isomerase, the main function is to convert D-glucose into D-fructose, and xylose into xylulose. The optimum pH is 6~8, manganese and potassium can improve heat resistance. The optimum temperature is 40~65 ℃(60 ℃). |
| Use | Use enzyme preparation, mainly used in the manufacture of high fructose syrup and other fructose starch syrup. Glucose Isomerase can catalyze D-glucose isomerization results of sugar, can also catalyze D-xylose into D-xylulose. China's provisions can be used for the preparation of high fructose corn syrup, according to the production needs of the appropriate amount of use. |
| Raw Materials | Benzene streptomyces avermifilis |
| EPA chemical information | Isomerase, glucose (9055-00-9) |
Toxicity
FAO/WHO 2001 stipulates that ADI is allowed to use glucose isomerase prepared by radiobacteria Missouri, Streptococcus olive, Streptomyces olive pigment, Bacillus coagulans (for immobilization); It is not specified for non-immobilized use made by Bacillus coagulans; ADI made by Streptomyces spaceosum does not make special provisions.
GRAS(FDA,& sect;184.1372,2000).
production method
1. the strains producing this enzyme include actinomycetes such as Streptomyces yellow, Streptomyces albicans (S.Allus) and brevis lactobacillus. This enzyme is an intracellular enzyme. The cultured bacteria are self-dissolved by hypertonic and transformed at 25 ℃ for 30h. After separation, the attachment of the culture solution is removed by water washing, and the product is prepared by spray drying or freeze drying./p>
2. Any of the variants of Bacillus coagulans (Bacillus coagulans), Streptomyces olive (Streptomyces olivaceus), Missouri pay-off trap (Actinoplanes missouriensis), Streptomyces olive pigment (Strp-tomycgs olivochromogenes), Streptomyces purple (Sreptomyces violaceoniger), Streptomyces brown (Streptomyces rubigi-nosus), and Aspergillus niger (AspPrgillusniger) are fermented under controlled conditions, because they are intracellular enzymes, therefore, the bacteria must be refined and dried by hypertonic autolysis (25 ℃,30h), acetone separation, etc.