Name | 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid Ammonium Salt |
Synonyms | ABTS ABTS-(NH4)2 ABTS SUBSTRATE ABTS DIAMMONIUM SALT ABTS(R), DIAMMONIUM SALT ABTS(TM) CHROMOPHORE, DIAMMONIUM SALT Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid Ammonium Salt 2,2'-AZINOBIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID AMMONIUM SALT) 2,2'-AZINO-BIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID) DIAMMONIUM SALT Diammonium 2,2'-azinobis(3-ethyl-2,3-dihydrobenzothiazole-6-sulphonate) 2,2'-Azino-di(3-ethyl-benzthiazoline sulphonic acid (6)), ammonium salt 6-Benzothiazolesulfonic acid, 2,2'-azinobis(3-ethyl-2,3-dihydro-, diammonium salt diammonium (2Z)-3-ethyl-2-[(2E)-(3-ethyl-6-sulfonato-1,3-benzothiazol-2(3H)-ylidene)hydrazono]-2,3-dihydro-1,3-benzothiazole-6-sulfonate |
CAS | 30931-67-0 |
EINECS | 250-396-6 |
InChI | InChI=1/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18+; |
Molecular Formula | C18H24N6O6S4 |
Molar Mass | 548.68 |
Melting Point | >181oC (dec.) |
Water Solubility | Dissolve in water at 50mg/ml. Solution may be slightly hazy. |
Solubility | Dissolve in water at 50mg/ml. Solution may be slightly hazy. |
Appearance | tablet |
Color | Pale Green to Light Green |
BRN | 7329461 |
PH | pH(50g/l, 25℃) : 5.0~6.0 |
Storage Condition | 2-8°C |
Stability | Hygroscopic, Light Sensitive |
Sensitive | Light Sensitive |
MDL | MFCD00010404 |
Use | This product is for scientific research only and shall not be used for other purposes. |
In vitro study | A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H 2 O 2 concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H 2 O 2 . Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 µM. |
Hazard Symbols | Xi - Irritant |
Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. |
WGK Germany | 3 |
RTECS | DL7002000 |
FLUKA BRAND F CODES | 8-10-23 |
HS Code | 29342080 |
biological activity | ABTS diammonium salt is a substrate for horseradish peroxidase (HRP). |
Use | A peroxidase matrix suitable for the ELISA step. This matrix produces a soluble green end product and can be used in a spectrophotometer Observed at 405nm; free chlorine spectroscopic reagent, enzyme-free chromogenic substrate peroxidase substrate free chlorine spectroscopic reagent, enzyme-free chromogenic substrate, peroxidase substrate, for the ELISA step, the substrate produces a soluble green end product that can be spectral read at 405 nm. This reaction can be terminated with 1% SDS. |
category | toxic substances |
flammability hazard characteristics | combustible; combustion produces toxic nitrogen oxides and sulfur oxide smoke |
storage and transportation characteristics | ventilation and low temperature drying |
fire extinguishing agent | dry powder, foam, sand, carbon dioxide, mist water |