Name | BAM15 |
Synonyms | BAM15 CS-2890 N,N'-bis(2-fluorophenyl)[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine [1,2,5]Oxadiazolo[3,4-b]pyrazine-5,6-diamine, N5,N6-bis(2-fluorophenyl)- BAM 15 (N5,N6-bis(2-Fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine) |
CAS | 210302-17-3 |
Molecular Formula | C16H10F2N6O |
Molar Mass | 340.29 |
Density | 1.548±0.06 g/cm3(Predicted) |
Boling Point | 421.7±55.0 °C(Predicted) |
Solubility | DMSO : 75 mg/mL (220.40 mM; Need ultrasonic)H2O : < 0.1 mg/mL (insoluble) |
Appearance | powder |
Color | white to beige |
pKa | -2.40±0.50(Predicted) |
Storage Condition | -20°C |
Stability | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month. |
Use | BAM 15 is a mitochondrial proton uncoupling agent. BAM 15 is an oxidative phosphorylation (OXPHOS) uncoupling agent. |
In vitro study | BAM 15 is able to increase O 2 consumption across a broad dosing range without increasing ROS. BAM 15 and FCCP are structurally unrelated and it is observed that low doses of BAM 15 from 100 nM to 1 μM increase cellular O 2 consumption rate (OCR) to a similar degree as FCCP, but higher concentrations from 1 μM to 50 μM reveal that BAM 15 is able to maintain uncoupled respiration at a high rate in a range of cell lines. BAM 15 is fully capable of increasing mitochondrial respiration in the presence of oligomycin and does so across a broader concentration range than FCCP in both myoblasts and hepatocytes. BAM 15 induces mitochondrial swelling, demonstrating that BAM 15 is a protonophore. BAM15-treated cells are more viable than FCCP-treated cells when administered across a broad dosing range up to 50 μM. |
In vivo study | Compare to vehicle-treated mice, animals that receive BAM 15 are protected from kidney injury as indicated by lower plasma creatinine levels at 24 and 48 h post-ischemia, reduced tubular necrosis, less depletion of brush border villi, less obstruction of proximal tubules, and less immune cell infiltration. |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 2.939 ml | 14.693 ml | 29.387 ml |
5 mM | 0.588 ml | 2.939 ml | 5.877 ml |
10 mM | 0.294 ml | 1.469 ml | 2.939 ml |
5 mM | 0.059 ml | 0.294 ml | 0.588 ml |
biological activity | BAM 15 is a novel uncoupling agent for the mitochondrial protonophore, it can protect mammals from acute renal ischemia-reperfusion injury and cold-induced microtubule injury. BAM 15 is also a potent activator of AMPK. BAM 15 reduces apoptosis caused by trafficking in iPS-differentiated retinal tissue. |
Target | Value |