BTB
Bromothymol Blue
CAS: 76-59-5
Molecular Formula: C27H28Br2O5S
BTB - Names and Identifiers
| Name | Bromothymol Blue |
| Synonyms | BTB ol)s,s-dioxide Bromthymol Blue Bromothymol Blue dibromothymolsulphophthalein dibromothymolsulphonephthalein 3,3-Dibromothymolsulfonephthalein 3,3-Dibromophenolsulfonephthalein 3,3′-Dibromothymothymolsulfonphthalein 6,6'-(3h-2,1-benzoxathiol-3-ylidene)bis(2-bromothymol)s,s-dioxide alpha,alpha-bis(5-bromo-4-hydroxy-6-methyl-m-cumenyl)toluene-2,alpha-sultone 4,4'-(3h-2,1-benzoxathiol-3-ylidene)bis(2-bromo-3-methyl-6-(1-methylethyl)phen 4,4'-(1,1-dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis[2-bromo-3-methyl-6-(propan-2-yl)phenol] PHENOL, 4,4'-(1,1-DIOXIDO-3H-2,1-BENZOXATHIOL-3-YLIDENE)BIS[2-BROMO-3-METHYL-6-(1-METHYLETHYL)- PHENOL, 4,4'-(3H-2,1-BENZOXATHIOL-3-YLIDENE)BIS[2-BROMO-3-METHYL-6-(1-METHYLETHYL)-, S,S-DIOXIDE 2-bromo-4-[3-(3-bromo-4-hydroxy-2-isopropyl-5-methyl-phenyl)-1,1-dioxo-benzo[c]oxathiol-3-yl]-3-isopropyl-6-methyl-phenol |
| CAS | 76-59-5 |
| EINECS | 200-971-2 |
| InChI | InChI=1/C27H28Br2O5S/c1-13(2)21-18(11-15(5)25(30)23(21)28)27(17-9-7-8-10-20(17)35(32,33)34-27)19-12-16(6)26(31)24(29)22(19)14(3)4/h7-14,30-31H,1-6H3 |
BTB - Physico-chemical Properties
| Molecular Formula | C27H28Br2O5S |
| Molar Mass | 624.38 |
| Density | 1.4668 (estimate) |
| Melting Point | 200-202 °C (lit.) |
| Boling Point | 614.3±55.0 °C(Predicted) |
| Flash Point | 38°C |
| Water Solubility | slightly soluble |
| Solubility | Solubility Sparingly soluble in water, benzene; soluble in ethanol, ether; insoluble in petroleum ether |
| Vapor Presure | 5.5E-17mmHg at 25°C |
| Appearance | Purple and pink powder |
| Color | Pink-brown to purple |
| Odor | Characteristic odour |
| Maximum wavelength(λmax) | ['420nm, 435nm, 620nm'] |
| Merck | 14,1445 |
| BRN | 373934 |
| pKa | 7.0, 7.1(at 25℃) |
| PH | pH : 6.0～7.6 |
| Storage Condition | Store at +5°C to +30°C. |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| Sensitive | Easily absorbing moisture |
| Refractive Index | 1.634 |
| MDL | MFCD00005872 |
| Physical and Chemical Properties | Bromothymol blue colorless or light rose crystalline powder, soluble in alcohol, dilute alkali solution and ammonia, slightly soluble in water, insoluble in petroleum ether. When it is acidic, it is yellow; When it is alkaline, it is blue. |
| Use | Used as acid-base indicator |
| In vitro study | A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of BTB as the pH indicator and potassium phosphate as the buffer component. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies. |
BTB - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | R10 - Flammable R41 - Risk of serious damage to eyes R36/37/38 - Irritating to eyes, respiratory system and skin. R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. |
| Safety Description | S24/25 - Avoid contact with skin and eyes. S39 - Wear eye / face protection. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. |
| UN IDs | 1170 |
| WGK Germany | 3 |
| RTECS | SJ450000 |
| TSCA | Yes |
| HS Code | 29349990 |
| Hazard Class | 3 |
| Packing Group | III |
BTB - Reference
| Reference Show more | 1. [IF=13.273] Xianghong Xie et al."A “sense-and-treat” hydrogel for rapid diagnose and photothermal therapy of bacterial infection."CHEMICAL ENGINEERING JOURNAL. 2022 Sep;443:136437 |
BTB - Introduction
Insoluble in water, easily soluble in ethanol, dilute alkali or ammonia solution.
Last Update：2022-10-16 17:28:28
BTB - Reference Information
| pH range of acid-base indicator discoloration | 6(yellow)-7.6(blue) |
| Main application | Sol-gel matrix, fuel cells, optical sensors, combustion gas detection system, paints, toys, cleaning products, detergens, textures, food freshness sensor, identifying fresh and stain rice,wheat, decting microorganizations, bacterial growth, psychactive drug, Dal materials |
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Introduction | bromothymol blue is an acid-base indicator with a color change range of pH6.0 (yellow) to 7.6 (blue). Ordinary water is neutral, pH is about 7, almost light green. |
| Use | bromothymol blue is a pH indicator of weak acids and weak bases, it is used as an acid-base indicator and a chromatographic analysis reagent in analytical chemistry; It can also be used as a dye and a metabolite. acid-base indicator, pH color change range 6.0 (yellow)-7.6 (blue). Adsorption indicator. |
| indicator color change range | pH color change range from 6.0 (yellow) to 7.6 (blue). |
| Application | • pH indicator-has precise color conversion at near-neutral, yellow at acidic, and blue at alkaline. • Stain-trace the movement of lymph fluid under a microscope to determine the cell wall or cell nucleus • Medium components-in agar gel medium for Bacillus cereus enumeration; As a bacterial growth component and detection substrate, for example, Cystine/lactose/electrolyte deficiency-BTB disk method-Emergency Determination of whole blood cholinesterase activity for the determination of organophosphate poisoning. |
| preparation | Method 1: bromine is introduced into a suspension of Thymol Blue in glacial acetic acid with stirring, the reaction was carried out at 50 °c to obtain bromothymol blue. Method 2: The Excess bromine and glacial acetic acid solution was added slowly to the aqueous acetic acid solution of Thymol blue under stirring, and the reaction was completed at 40~50 ℃ for 4H, it was cooled to below 20 °c, filtered and dried to give colorless bromothymol blue. |
| biological activity | Bromothymol Blue is a pH indicator used for the determination of weak acids and bases, when it is acidic, it is yellow, and when it is alkaline, it is blue. |
Last Update：2024-04-09 20:52:54
Supplier List
Spot supply
Product Name: Bromothymol Blue Request for quotationCAS: 76-59-5
Tel: +86-17551318830
Email: r@reformchem.com
Mobile: +86-17551318830
QQ: 3787852685
Spot supply
Product Name: Bromothymol Blue Request for quotationCAS: 76-59-5
Tel: +86-17551318830
Email: r@reformchem.com
Mobile: +86-17551318830
QQ: 3785839865
Product Name: Bromothymol Blue Request for quotation
CAS: 76-59-5
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 0086 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
CAS: 76-59-5
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 0086 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
Spot supply
Product Name: Bromothymol Blue Visit Supplier Webpage Request for quotationCAS: 76-59-5
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
Product Name: Bromothymol Blue Request for quotation
CAS: 76-59-5
Tel: 13998106873
Email: 297303255@qq.com
Mobile: 13998106873
QQ: 297303255
Send QQ message
CAS: 76-59-5
Tel: 13998106873
Email: 297303255@qq.com
Mobile: 13998106873
QQ: 297303255
Spot supply
Product Name: Bromothymol Blue Request for quotationCAS: 76-59-5
Tel: +86-17551318830
Email: r@reformchem.com
Mobile: +86-17551318830
QQ: 3787852685
Spot supply
Product Name: Bromothymol Blue Request for quotationCAS: 76-59-5
Tel: +86-17551318830
Email: r@reformchem.com
Mobile: +86-17551318830
QQ: 3785839865
Product Name: Bromothymol Blue Request for quotation
CAS: 76-59-5
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 0086 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
CAS: 76-59-5
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 0086 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
Spot supply
Product Name: Bromothymol Blue Visit Supplier Webpage Request for quotationCAS: 76-59-5
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
Product Name: Bromothymol Blue Request for quotation
CAS: 76-59-5
Tel: 13998106873
Email: 297303255@qq.com
Mobile: 13998106873
QQ: 297303255Send QQ message
CAS: 76-59-5
Tel: 13998106873
Email: 297303255@qq.com
Mobile: 13998106873
QQ: 297303255
View History