CINOBUFAGIN(P)
cinobufagin venom toad
CAS: 470-37-1
Molecular Formula: C26H34O6
CINOBUFAGIN(P) - Names and Identifiers
| Name | cinobufagin venom toad |
| Synonyms | cinobufagin CINOBUFAGIN(P) Cinobufagin std. RARECHEM BK HC T302 TRANS-CINNAMYLIC ACID cinobufagin venom toad TRANS-3-PHENYLPROPENOIC ACID TRANS-3-BENZENEPROPENOIC ACID 5b,20(22)-Bufadienolide-3b,16b-diol-14,15b-epoxy 16-acetate (3beta,5beta,15beta,16beta)-16-(acetyloxy)-3-hydroxy-14,15-epoxybufa-20,22-dienolide (3beta,5beta,8xi,9xi,10xi,13xi,14beta,15beta,16beta,17beta)-3-hydroxy-17-(2-oxo-2H-pyran-4-yl)-14,15-epoxyandrostan-16-yl acetate |
| CAS | 470-37-1 |
| EINECS | 636-927-8 |
| InChI | InChI=1/C26H34O6/c1-14(27)31-22-21(15-4-7-20(29)30-13-15)25(3)11-9-18-19(26(25)23(22)32-26)6-5-16-12-17(28)8-10-24(16,18)2/h4,7,13,16-19,21-23,28H,5-6,8-12H2,1-3H3/t16-,17+,18+,19-,21+,22-,23-,24+,25-,26-/m1/s1 |
CINOBUFAGIN(P) - Physico-chemical Properties
| Molecular Formula | C26H34O6 |
| Molar Mass | 442.55 |
| Density | 1.261 g/cm3 |
| Melting Point | 133°C(lit.) |
| Boling Point | 300°C(lit.) |
| Flash Point | >230°F |
| Solubility | Easily soluble in chloroform, methanol and other organic solvents, insoluble in water. |
| Vapor Presure | 0mmHg at 25°C |
| Appearance | White needle crystal |
| Color | White to Light yellow |
| pKa | 15.10±0.70(Predicted) |
| Storage Condition | 2-8°C |
| Refractive Index | 1.595 |
| MDL | MFCD00056825 |
| Physical and Chemical Properties | Soluble in chloroform, methanol and other organic solvents, insoluble in water. Dried secretions from Bufo bufo gargargiizans Cantor of the Bufo bufo Bufo bufo derived from animals of the toad family, for example |
CINOBUFAGIN(P) - Risk and Safety
| Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R26/27/28 - Very toxic by inhalation, in contact with skin and if swallowed. R42/43 - May cause sensitization by inhalation and skin contact. R20/22 - Harmful by inhalation and if swallowed. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| UN IDs | UN 2811 6.1/PG 1 |
| WGK Germany | 3 |
| RTECS | GD7850000 |
| HS Code | 29322090 |
| Hazard Class | 6.1(a) |
| Packing Group | II |
CINOBUFAGIN(P) - Reference
| Reference Show more | 1. Niutianli, the state of Qin. Inhibitory effect of cinobufagin on proliferation of human prostate cancer PC3 cells in vitro [J]. Journal of Beijing University of Chinese Medicine, 2018, 41(12):53-58. 2. Li Zhidong, Zhu Shichun, GE Zhaohui. HPLC Determination of different batches of Shexiang Baoxin pills Chinese toad venom base and xenopan venom ligand content [J]. Strait pharmacy 2017 029(012):72-74. 3. Zhang Mei Li Yu, Sangye Xiaoxue. Study on plasma Medicinal Chemistry of Liushen pills [J]. Green Science and Technology 2017 000(012):237-240. 4. Zhang Mei-Tuo et al. [IF = 2.193]. "Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar compositions in Traditional Chinese Medicines by HPLC." J Anal Methods Chem. 2018;2018:7569283 5. [IF = 4.411] Yueting Cao et al."Chemical Profile and Multicomponent Quantitative Analysis for the Quality Evaluation of Toad Venom from Different Origins."Molecules. 2019 Jan;24(19):3595 6. [IF=4.379] Cao Yueting et al."The impact of multiple climatic and geographic factors on the chemical defences of Asian toads (Bufo gargarizans Cantor)."Sci Rep-Uk. 2019 Nov;9(1):1-11 7. [IF=1.902] Ke-ke Cui et al."Quantitative analysis of multicomponents by a single marker and quality evaluation of Venenum Bufonis from different geographical origins."Biomed Chromatogr. 2019 Sep;33(9):e4555 |
CINOBUFAGIN(P) - Reference Information
| Source | Toad venom, also known as toad eyebrow fat, toad paste, toad paste, toad paste, is made of toad animals A white slurry secreted by the posterior ear glands of the Chinese toad or black-orbital toad is processed and dried. Capture in summer and autumn, wash, dry the water on the body surface, and then squeeze out the slurry secreted by the posterior ear glands and skin glands, and place it in porcelain. Avoid contact with iron, otherwise it will turn black. Filter out sediment and impurities, take pure pulp and put it into a round model to dry, which is called "lump crisp" (lump crisp). If the slurry is evenly coated on the glass plate or dried on the bamboo, it is called "crisp". Tuansu (Tuansu) is oblate, block-shaped, cake-shaped or chess piece-shaped, with brown, purple-black or purple-red surface, smooth, thin edges, slightly thick in the middle, 3 ~ 7cm in diameter, and some with wrinkles. Tough, not easy to break, brown or purplish red section, translucent glial, slightly shiny. When exposed to water, it will be milky white foam bulging, wrapped in tin foil and burned, and then dissolved into oil. The gas is slightly fishy, the taste is spicy, and the powder sniffs sneeze. The venom (venom) is rectangular or irregular thin slices, with different sizes and thiceness. The surface is tea brown or brown-black, translucent, rough on one side and smooth on the other. Brittle, easy to break, uniform section, slightly shiny. The air is slightly fishy and spicy. It is mainly produced in Hebei, Shandong, Jiangsu, Zhejiang and other provinces. |
| pharmacological effects | 1. cardiotonic effect: toad venom has a cardiotonic effect, which can strengthen the contraction of isolated toad heart in small doses; large doses stop the heart in systolic phase, and its effect is similar to digitalis. There are many kinds of cardiac components. And can raise blood pressure and excited breathing. 2. local anesthesia: toad venom 80% alcohol extract has surface anesthesia effect. in rabbit cornea and human tongue tests, the effect is slower and longer than caine, and has local stimulation effect. Bufalin has the strongest local anesthetic effect in toad venom, which is 90 times that of cocaine. 3. analgesic effect: toad venom has analgesic effect on experimental pain in mice. 4. the effect on white blood cells: toad venom has the effect of increasing the white blood cells caused by radiation. 5. Anti-inflammatory effect: Toad venom has no antibacterial effect in vitro. For rabbits infected with Staphylococcus aureus and Streptococcus, intramuscular injection of Toad venom can prevent the spread of lesions and make the surrounding redness and swelling subside. Anti-inflammatory effects are similar to hormones. Toad venom has the effect of anti-tuberculosis bacilli, and is effective in clinical treatment of lymphatic tuberculosis, tuberculosis and tuberculous fistula. 6. anticancer effect: toad skin extract has inhibitory effect on mouse sarcoma 180 and rabbit B. p tumor. Clinical use is effective in treating skin cancer. 7. diuretic effect: toad venom has diuretic effect, the effective ingredient is cork acid. Clinical treatment of ascites is effective. 8. antitussive effect: toad venom has antitussive and antiasthmatic effect on experimental cough and wheezing in mice. 9. Toxic effect: Venenum toad injection or intraperitoneal injection of toxic reaction, make the mice appear shortness of breath, muscle spasm, arrhythmia, finally paralysis and death. Atropine has a certain detoxification effect. The toxicity of toad venom is greatly reduced after boiling, and there is no accumulation effect. |
| clinical application | toad venom is widely used, can be taken internally and externally, and its dosage is small and powerful. 1. Carbuncle and furuncle poison: Toad venom is often used to treat carbuncle, malignant sores, nameless swelling and poison, hair and back, etc., which is similar to the beginning of various purulent infections, such as carbuncle, Boils, acute cellulitis, etc., it is effective to apply the affected area with grinding alone, and it can also be combined with light powder, cinnabar, realgar, Chuanwu, blood exhaust, etc, examples of "Chansu Pills", "Chansu Mixture", "Li Gong Indongzi", "Chemical Pill", "Chansu San", "Chansu Tori Pills". It is effective to treat boils, internal furs and swelling toxins, similar to acute lymphangitis, etc. It is effective alone. The prescription is "Longgu San". The treatment of scrofula can be combined with white clove, cold water stone, croton, etc. 2. Larynx swelling and pain: Toad venom is often used for sore throat, milk moth, rotten throat, throat pain, etc. caused by exogenous wind-heat, similar to acute angina, acute tonsillitis, scarlet fever angina, laryngitis, etc. Often compatible with musk, cinnabar, bezoar, pearl, etc., the prescriptions are "Liushen Pills" and "Blow Dissipation". 3. Local numbness and toothache: Toad venom can be used as a local anesthetic, used for tooth extraction, abscess incision and nasal polyp removal, etc., external use of "anesthetic application", oral "Qiongsu San". Single use can be used to treat dental caries pain, and can also be used with acunculus, Artemisia, Asarum, borneol, etc. Prescription "Yatong Pill" and "Toansu Paste". 4. Jue out of coma: Toad venom can be used for abdominal pain, diarrhea, coma, etc. caused by fulminant vomiting, similar to dehydration and acidosis caused by acute gastroenteritis. It can be compatible with aphrodisiac, clove, musk, cinnabar and other resuscitation and refreshing drugs, the prescription is "Chansu Pills"; in recent years, it has been used to treat heart palpitations and collapse caused by heart failure, using Chansu 4-8 mg alone, taken in capsules, 2-3 times a day. 5. Tuberculosis tumor: Toad venom has been used to treat infantile malnutrition in the past dynasties, similar to emaciation caused by tuberculosis, and can be combined with cinnabar, musk, etc. as pills. Recently used to treat bone and joint tuberculosis, chronic osteomyelitis and fistula formation, etc., you can take "Toad venom Tablets", 5-10 mg each time, 3 times a day, after meals, and take it until the fistula is closed. Take it for 2 months to consolidate the curative effect. Inject 0.2 ~ 0.5% toad venom solution into the fistula and keep it for 1 hour, once a day, until the fistula is closed. 20% Toad venom ointment is effective in treating skin cancer. It can be healed in about 18 days after dressing change once every 3 days. In addition, the ancient prescriptions are still used to treat tetanus, and most of them are compatible with scorpion and gastrodia. Prescription "Toad Scorpion Pills"; recently used to treat leukopenia and liver cirrhosis ascites. Reference material: Edited by Xiong Fuxin. Dictionary of clinical traditional Chinese medicine. Kunming: Yunnan Science and Technology Press. |
| Processing research | In the Song Dynasty, there were still iron baking coke ("Taiping Shenghui Fang"), refining, wine making, and soaking ("Children's Health General Micro-prescription") And other methods. experiments show that toad venom wine is easy to crush after being soaked and dried. The composition of toad venom did not change before and after wine preparation, but the total cardiac steroid content increased after wine preparation. It is believed that wine production is not only conducive to crushing, but also enhances the curative effect. Taking the active ingredient of toad venom as an index, the content of raw products, wine products, and dairy products is determined and compared. The result is that the raw products are higher than wine products. The products are higher than dairy products. The three thin-layer chromatographic behaviors are basically the same, but the front of dairy products has more spots. the toxicity comparison results of different processed products show that the traditional wine preparation method is economical and time-saving, and the standard is easy to master. In other experimental studies, the cyclohexane-chloroform-acetone (4:3:3) system is the best for the separation of bufolactone. The determination of the content of butotoxide components by thin-layer scanning method can separate the lactone components from the interfering substances without being affected. Compared with the original medicinal materials, the area score of the main lactone component and the content of the single component ester toad poison base are significantly reduced. The content is the original medicinal materials> wine products> talcum powder products, indicating that the processing can make toad poison The lactone component is reduced, which may reduce the efficacy. Reference: Ye Dingjiang, the original editor-in-chief of Sitong. Dictionary of Chinese Medicine Processing. Shanghai: Shanghai Science and Technology Press. |
| use | used for content determination/identification/pharmacological experiments, etc. Pharmacological effects: anti-tumor effect, cardiotonic effect, antiarrhythmic and analgesic effect. |
| category | toxic substances |
| toxicity classification | highly toxic |
| acute toxicity | oral administration-mouse LD50: 144 mg/kg; Intravenous-mouse LD50: 1.21 mg/kg |
| flammability hazard characteristics | combustible, spicy and irritating smoke from the fire site |
| storage and transportation characteristics | warehouse is low temperature (below 4 ℃), ventilated and dry; Store separately from food raw materials |
| fire extinguishing agent | water, carbon dioxide, dry powder, sand |
Last Update：2024-04-09 20:52:54
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Spot supply
Product Name: Cinobufagine Visit Supplier Webpage Request for quotationCAS: 470-37-1
Tel: 0714-3999186
Email: 2853786052@qq.com
Mobile: 86+15671228036
QQ: 2853786052
Wechat: 15671228036
Multiple SpecificationsSpot supply
Product Name: Cinobufagin Visit Supplier Webpage Request for quotationCAS: 470-37-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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