Campesterin
CAMPESTEROL
CAS: 474-62-4
Molecular Formula: C28H48O
Campesterin - Names and Identifiers
| Name | CAMPESTEROL |
| Synonyms | Campesterin CAMPESTEROL 24alpha-Methylcholesterol Ergost-5-en-3beta-ol, (24R)- DELTA5-24-Isoergosten-3beta-ol (24R)-Methylcholest-5-en-3beta-ol |
| CAS | 474-62-4 |
| EINECS | 207-484-4 |
Campesterin - Physico-chemical Properties
| Molecular Formula | C28H48O |
| Molar Mass | 400.68 |
| Density | 0.98±0.1 g/cm3 (20 ºC 760 Torr) |
| Melting Point | 156-160 °C |
| Boling Point | 463.29°C (rough estimate) |
| Specific Rotation(α) | D23 -33° (22.5 mg in 5 ml chloroform) |
| Solubility | Easily soluble in chloroform, hardly soluble in methanol. |
| Appearance | White powder |
| Storage Condition | -20℃ |
| Refractive Index | 1.51 |
| MDL | MFCD00010475 |
| Physical and Chemical Properties | Chemical properties of white crystalline powder, soluble in methanol, ethanol, DMSO and other organic solvents, from light bamboo leaves and rhizomes (grass) rape seeds, flue-cured tobacco leaves, Burley tobacco leaves, corn silk. |
| Use | Uses for content determination/identification/pharmacological experiments and other pharmacological effects: lower cholesterol, anticancer and other activities |
Campesterin - Risk and Safety
| Risk Codes | R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. R36/37/38 - Irritating to eyes, respiratory system and skin. R48/20/22 - R40 - Limited evidence of a carcinogenic effect R38 - Irritating to the skin R22 - Harmful if swallowed |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S23 - Do not breathe vapour. |
| UN IDs | UN 1888 6.1/PG 3 |
Campesterin - Reference
| Reference Show more | 1. Fu tiokun, Zhou Wei, Xia Wen, etc. Study on extraction technology of three main sterols from pitaya stem [J]. Journal of Tropical Agriculture Sciences, 2019, v.39;No.268(12):46-54. 2. Qin Guo et al [IF = 7.514]. "Action of physisterols on thermally induced trans fatty acids in pea oil." Food Chem. 2021 May;344:128637 3. [IF = 5.923] Lixiu Hou et al."Genome-Wide Identification of CYP72A Gene Family and Expression Patterns Related to Jasmonic Acid Treatment and Steroidal Saponin Accumulation in Dioscorea zingiberensis."Int J Mol Sci. 2021 Jan;22(20):10953 4. [IF=4.032] Qiu-yue Xiao et al."A network pharmacology-based study on key pharmacological pathways and targets of Qi Fu Yin acting on Alzheimer's disease."Exp Gerontol. 2021 Jul;149:111336 5. [IF=4.766] Meng Qin et al.Misdiagnosis of sitosterolemia in a patient as Evans syndrome and familial hypercholesterolemia.J Clin Lipidol. 2021 Nov;: 6. [IF=7.514] Simin Feng et al."Simultaneous analysis of free phytosterols and phytosterol glycosides in rice bran by SPE/GC-MS."Food Chem. 2022 Sep;387:132742 7. [IF=7.514] Bin Li et al."Enzyme-assisted ReMALDI-MS assay for quantification of cholesterol in food."Food Chem. 2022 Jul;383:132444 8. [IF=4.556] Junpeng Zeng et al."The comparative analysis of different oil extraction methods based on the quality of flaxseed oil."J Food Compos Anal. 2021 Dec;:104373 9. [IF=5.753] Jianghong Gao et al."Molecular Cloning and Functional Characterization of a Sterol 3-O-Glucosyltransferase Involved in Biosynthesis of Steroidal Saponins in Trigonella foenum-graecum.."Front Plant Sci. 2021 Dec;12:809579-809579 |
Campesterin - Biosynthesis of campesterol by recombinant Saccharomyces cerevisiae
from VIP Journal Professional Edition
Author:
Zhou Wulin , High , Yuling Wu , Display , Xu Meijuan , Yang Shuwei , Shao Minglong , Rao Zhiming
Abstract:
As an important synthetic precursor of steroid drugs (progesterone, androstenedione, hydrocortisone, etc.), campesterol has received extensive attention from researchers at home and abroad. Firstly, through bioinformatics analysis, 10 kinds of different sources of 7-dehydrocholesterol reductase DHCR7 were screened, and the use of CRISPR/Cas9 gene editing technology the endogenous ERG5 gene was replaced by DHCR7 gene from different sources, and the campesterol-synthesizing strain was constructed. As a result, it was found that the strain Zw507, which integrated the Pangasianodon effected DHCR7, exhibited the highest production of campesterol of 216.93 mg/L. Ten yeast promoters with strong endogenous promoter strength were further screened to combine with PhDHCR7 gene. The results showed that the yield of campesterol could reach 253.35 mg/L when TEF1p was used as promoter. In order to further improve the yield of campesterol, the copy number of DHCR7 expression cassette in yeast genome was increased. When the copy number was 3, the yield of campesterol reached the highest 302.27 mg/L. Finally, the yield of 916.88 mg/L campesterol was achieved by fed-batch fermentation in 5 L fermentor. The strain can be used as an excellent chassis cell for subsequent steroid biosynthesis.expand
Key words:
7-dehydrocholesterol reductase Saccharomyces cerevisiae campesterol synthetic biology fed-batch fermentation
year:
2021
Last Update：2024-01-02 23:10:35
Campesterin - Separation of β-sitosterol and campesterol by high-speed countercurrent chromatography
from VIP
Author:
Zhou Yujie , Dai Lingmei , fuming Chen , Li General Cheng
Abstract:
using high-speed countercurrent chromatography, and choose V (heptane):V (acetonitrile):V (ethyl acetate) = 5:5:1 solvent system, Β-sitosterol and campesterol in mixed phytosterol standard were successfully separated. Through HPLC detection, β-sitosterol with mass fraction of 97% and campesterol with mass fraction of 91% can be obtained by one-time separation, and the yield can reach 56% and 50% respectively. The method is simple and reproducible, and can be used as the preparation and separation method of high purity β-sitosterol and campesterol standard.
Key words:
high-speed countercurrent chromatography β-sitosterol campesterol detach purify
DOI:
10.3321/j.issn:1003-5214.2002.03.020
cited:
year:
2002
Last Update：2023-08-16 22:09:08
Campesterin - Method for separating and determining campesterol, stigmasterol and β-sitosterol by high performance liquid chromatography
from Baidu Library
Application (patent) number:
CN201711316125.5
application date:
2017-12-12
Public/Announcement Number:
CN107782828A
Public/announcement date:
2018.03.09
applicant (patent):
Jiangnan University
inventor:
Changming , Feng , Wang Xingguo , Jin Qingyi , Liu Ruijie , Wu Zhengzhang , Zhang Peng
National and provincial code:
CN320211
Abstract:
The invention discloses a method for separating and determining campesterol, stigmasterol and β-sitosterol by high performance liquid chromatography, the mixed standard stock solution containing campesterol, stigmasterol and β-sitosterol was prepared by polar solvent. After treatment, the mixed standard series solutions containing campesterol, stigmasterol and β-sitosterol were prepared by polar solvent. Our invention not only makes the three kinds of sterol monomers to achieve baseline separation, but also greatly shortens the retention time. Finally, the optimal chromatographic conditions are determined as 1.0mL/min and the detection wavelength is 208nm, the column temperature was 35 ℃, and the precision of the method and the sample recovery rate were higher, which could meet the requirements of accurate quantitative determination of three kinds of sterol monomer content.
Last Update：2023-08-16 22:09:08
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