Molecular Formula | C55H72MgN4O5 |
Molar Mass | 893.49 |
Melting Point | 117-120° |
Boling Point | 1032.1°C at 760 mmHg |
Specific Rotation(α) | D20 -262° (acetone) |
Flash Point | 578°C |
Solubility | Soluble in ethanol: 10ug/ml |
Vapor Presure | 0mmHg at 25°C |
Appearance | Dark blue or dark green powder |
Color | Green to Black Semi-Solid to |
Merck | 13,2174 |
BRN | 3586237 |
Storage Condition | -20°C |
Stability | Stable, but may discolour upon exposure to light. Incompatible with strong oxidizing agents. May be air sensitive. |
Sensitive | Sensitive to light and air |
MDL | MFCD00079050 |
Physical and Chemical Properties | Waxy solid. Olive-green to dark-green, depending on the amount of magnesium incorporated. Slightly abnormal odor. The melting point of chlorophyll a was 150~153 ℃, and the melting point of chlorophyll B was 183~185 ℃. It co-exists in the chloroplasts of plant leaves with the yellow chlorophyll B and lutein. Sensitive to light and heat, in dilute alkali solution can be saponified and hydrolyzed into green chlorophyllin (salt), chlorohydrins and methanol, in acid solution can be dark green to green Brown magnesium chlorophyll. Insoluble in water. Soluble in ethanol, ether, acetone and other fat solvents. |
Use | Can be used as soap, fat, wax, food, cosmetics and pharmaceutical non-toxic colorants |
Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
WGK Germany | 3 |
RTECS | FW6420000 |
FLUKA BRAND F CODES | 8-10-23 |
HS Code | 29339900 |
Reference Show more | 1. Tian xuetin Wu Jiao Wang Lei et al. Effects of 1-MCP treatment on the chlorophyll degradation of postharvest 'macadamia Apple '[J]. Journal of fruit science 2020 v.37(05):122-130. 2. Zhou Fuhui Xu Dong Ying left in China et al. Effect of LED white light treatment on quality of postharvest lettuce [J]. Packaging Engineering 2020 v.41;No.419(05):17-23. 3. Lu, Caicai, et al. "Chlorophyll as natural redox mediators for the denituration process." International biodeterication & biodegrading 148 (2020): 104895.https://doi.org/10.1016/j.ibiod.2020.104895 4. [IF = 4.5] Yinfei Liu et al. "Biochemical and functional characterization of AcUFGT3a, a galactosyltransferase involved in anthocyanin biosynthesis in the red-fleshed kiwifruit (Actinidia chinensis)."Physiol Plantarum. 2018 Apr;162(4):409-426 5. [IF=9.297] Yingzheng Yao et al."Silicon dioxide as an efficient adsorbent in the degumming of rapeseed oil."J Clean Prod. 2020 Sep;268:122344 6. [IF=8.34] Zhu Kaijie et al."Regulation of carotenoid and chlorophyll pools in hesperidia anatomically unique fruits found only in Citrus."Plant Physiol. 2021 Oct;187(2):829-845 7. [IF=4.32] Caicai Lu et al."Chlorophyll as natural redox mediators for the denitrification process."Int Biodeter Biodegr. 2020 Mar;148:104895 |
EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
Overview | Chlorophyll A is an important pigment contained in many pigments of phytoplankton. In phytoplankton, it accounts for 1% [KG-1.3mm] ~ 2% of the dry weight of organic matter, which is an index for estimating primary productivity and biomass, and is also a necessary item for red tide monitoring. |
preparation method | the specific steps of the preparation method of high purity chlorophyll a are as follows: take 1L of spirulina platensis solution grown to the late logarithmic growth stage, centrifuge the algal body, discard the supernatant, precipitate and enrich in a centrifuge tube, then extract it three times with 50mL ethanol, and merge the extracts. Add appropriate amount of petroleum ether (60~90 ℃) to the extract, add water to separate the phase, and wash the pigment-containing petroleum ether phase twice with water. Fill the chromatography column with 100-200 mesh neutral alumina, first wet the alumina column with petroleum ether solution containing 0.3% ethanol, then load the sample, and then use 0.3%, 10% ethanol-petroleum ether mixed solvent gradient rinse. The first is orange-yellow carotenoids, and then the blue-green chlorophyll. The solvent was recovered by spin evaporation to obtain dark green chlorophyll a precipitate. |
determination method | fluorescence spectrophotometry was used to extract pigment with acetone, and fluorescence measurement was carried out. the contents of chlorophyll a and magnesium removal pigment were calculated according to the fluorescence value before and after acidification of the extract. This method requires a small amount of samples and high sensitivity, which can eliminate the interference of magnesium removal pigment. The ratio of chlorophyll a to magnesium pigment can measure the physiological state of phytoplankton. |
use | edible green pigment. Used for pastries, beverages, liqueurs, etc. In fact, plant leaves or dry powder are often used directly. For example, tea powder, mugwort, spinach, chlorella, etc. Adding to gum sugar can eliminate halitosis. Chlorophyll is used to color soap, mineral oil, wax and essential oil. Chlorophyll or chlorophyllin derivatives, such as copper chlorophyllin [11006-34-1], sodium iron chlorophyllin, sodium copper chlorophyllin, used in food, candy, beverages, toothpaste, etc. as colorants and deodorants. Chlorophyllin derivatives can be used as a formula for smelly cosmetics with fungicides Jieermite, halocaban, etc. Can be used as a non-toxic colorant for soap, fat, oil wax, food, cosmetics and medicine |
production method | mostly uses plants (such as spinach, etc.) or dried silkworm sand as raw materials to extract chlorophyll. For example, the following methods can be used to extract chlorophyll from silkworm sand. Organic solvent extraction: clean silkworm sand is taken, mixed with more than 70% industrial ethanol into slurry, filtered out dark green solution, and then dried. Another operation is to mix 45 parts of petroleum ether, 15 parts of methanol and 4 parts of benzene, mix and mix with clean silkworm sand, filter and wash the filtrate 4 times. Add a small amount of sodium sulfate to the organic extract to remove residual water, filter, and recover the solvent from the filtrate to obtain chlorophyll. Physical separation: 0.4mol/L dilute sucrose solution and 0.06mol/L dilute potassium phosphate solution (pH 6-7) are mixed into buffer medium according to 1:1. Add about 1L of buffer medium per 2kg of silkworm sand, mix evenly, filter out green suspension with multi-layer emery cloth, centrifuge in a low-temperature centrifuge for 5-10min, wash the precipitate with water, and centrifuge again to obtain chlorophyll precipitate. Chlorophyll is treated with oxalic acid to obtain magnesium-free pheophyllin, and then magnesium is introduced back into chlorophyll. Remove phytol and magnesium with strong acid to obtain magnesium removal chlorophyllic acid, phytol can also be reintroduced. Hydrolysis removes phytol and methanol to obtain chlorophyllin. Most plants (such as spinach, etc.) or dried silkworm sand are used as raw materials to extract chlorophyll. For example, the following methods can be used to extract chlorophyll from silkworm sand. Organic solvent extraction: clean silkworm sand is taken, mixed with more than 70% industrial ethanol into slurry, filtered out dark green solution, and then dried. Another operation is to mix 45 parts of petroleum ether, 15 parts of methanol and 4 parts of benzene, mix and mix with clean silkworm sand, filter and wash the filtrate 4 times. Add a small amount of sodium sulfate to the organic extract to remove residual water, filter, and recover the solvent from the filtrate to obtain chlorophyll. Physical separation: 0.4mol/L dilute sucrose solution and 0.06mol/L dilute potassium phosphate solution (pH 6-7) are mixed into buffer medium according to 1:1. Add about 1L of buffer medium per 2kg of silkworm sand, mix evenly, filter out green suspension with multi-layer emery cloth, centrifuge in a low-temperature centrifuge for 5-10min, wash the precipitate with water, and centrifuge again to obtain chlorophyll precipitate. Chlorophyll is treated with oxalic acid to obtain magnesium-free pheophyllin, and then magnesium is introduced back into chlorophyll. Remove phytol and magnesium with strong acid to obtain magnesium removal chlorophyllic acid, phytol can also be reintroduced. Hydrolysis removes phytol and methanol to obtain chlorophyllin. Use organic solvents (acetone, butanol, dichloromethane, ethanol, petroleum ether, isopropanol, trichloroethylene, butanone) to extract the leaves of clover, daisy, spinach, cabbage, nettle, etc. Or it is obtained by extracting the by-products of corn long-whisker products, followed by removing (or not removing) the magnesium in the complex and removing the solvent. |