Molecular Formula | C18H38NO5P
|
Molar Mass | 379.472 |
Density | 1.094±0.06 g/cm3(Predicted) |
Melting Point | 179-189℃ |
Boling Point | 548.8℃ at 760 mmHg |
Solubility | Soluble in methanol (1 mg/ml), warm acetic acid, 0.3M NaOH (~4 mg/ml), and methanol: T |
Appearance | grayish white to gray powder |
Color | yellow |
pKa | 1.76±0.10(Predicted) |
Storage Condition | -20℃ |
Stability | Moisture, Temperature Sensitive; Hygroscopic |
Sensitive | `sensitive` to light, air and humidity |
Physical and Chemical Properties | Bioactive Sphingosine-1-phosphate (S1P) is an agonist of S1P1-5 receptors and a ligand for GPR3, GPR6, gpr12. Sphingosine-1-phosphate is an intracellular second messenger that mobilizes Ca2 as an extracellular ligand for G protein-coupled receptors. Sphingosine-1-phosphate is an important lipid mediator produced by sphingomyelin (HY-113498) or other membrane phospholipids. |
Use | A ligand for EDG-1 and EDG-3 and activator of GPR3, GPR6, and GPR12. |
Target | Human Endogenous Metabolite |
In vitro study | S1P (1 μM) induces a significant Ca 2+ releases in HEK293 cells under serum starvation conditions (1% FCS).In a functional Ca 2+ assay, Suramin (HY-B0879) alone does not exert any effect on intracellular Ca 2+ release via gpr3, gpr6 or gpr12. In contrast, S1P (1 μM) induces Ca 2+ release of gpr3, gpr6 and gpr12 in the presence of Suramin (HY-B0879) various concentrations in transfected HEK293 cells. In a functional Ca 2+ assay,S1P (3-3000 nM) in the presence Suramin (300 μM), exhibits nanomolar EC 50 values for gpr3 (EC 50 =29 nM), gpr6 (EC 50 =15 nM) and gpr12 (EC 50 =24 nM), rat gpr3 (EC 50 =68 nM),respectively in HEK293 cells. S1P increases intracellular calcium levels in TAg-Jurkat cells expressing S1P1 and G qi5 , which allows for phospholipase C stimulation by G i proteins, when used at a concentration of 200 nM, as well as in TAg-Jurkat cells expressing S1P2 and S1P3 receptors (EC 50 s = 8 and 11 nM, respectively). |