| Name | Deoxynucleotide |
| Synonyms | Deoxynucleotide deoxyribonucleotide COMPOSITE SODIUM5-DEOXYRIBONUCLEOTIDE |
| Physical and Chemical Properties | Chemical quality This product is yellow or light brown flake or powder; strong hygroscopicity. |
| Use | Use can enhance bone marrow hematopoietic function. Clinical for the treatment of acute and chronic hepatitis, leukopenia. |
background and overview
Chronic viral hepatitis B is a common clinical infectious disease. It has the characteristics of strong infectivity, complex transmission routes, and high incidence. If effective treatment is not available, it can develop into liver cirrhosis and liver cancer. It endangers the lives of patients and does great harm. Abnormal liver function is a common complication of chronic hepatitis B. How to control the development of the disease and effectively restore liver function is a hot topic in clinical treatment at present. The treatment principles of chronic hepatitis B mainly include antiviral, immune regulation, anti-inflammatory and liver protection, anti-fibrosis and symptomatic treatment. Antiviral therapy is the key, but it is also important to relieve liver inflammation, promote liver cell regeneration and immune regulation therapy. Interferon and nucleoside (acid) analogs are two first-line drugs for the treatment of chronic hepatitis B. In recent years, with the continuous advent of new drugs, there are new options for the treatment of chronic hepatitis B.
Deoxynucleotide (Deoxyribonucleotide) is a compound formed by connecting nitrogen-containing bases (purine or pyrimidine), deoxyribose and phosphoric acid. It is a constituent unit of deoxyribonucleic acid, including deoxyguanylate, Deoxyadenosine acid, deoxycytidine acid and deoxythymidine acid. Sodium deoxynucleotide injection is based on deoxyribonucleic acid extracted by biotechnology as raw material, formed by the action of a variety of biological enzymes, rich in adenine nucleotides, guanine nucleotides cytosine nucleotides, thymine nucleotides and other four nucleotide components, participate in the process of nucleic acid metabolism in the body, and have a wide range of pharmacological effects on multiple organs and systems such as the liver, heart, blood system. According to clinical reports, the efficacy of deoxynucleotide and Bozhi glycopeptide alone in the treatment of chronic hepatitis B is equivalent to that of interferon alone. Nucleic acid is a necessary nutrient for maintaining normal cellular immunity. Since the immune system does not have the ability to synthesize nucleotides from scratch, supplementing exogenous nucleotides can effectively promote the body to maintain normal immune function. Sodium deoxynucleotide can regulate the function of peripheral lymphocytes, promote the secretion of interleukin 2 and d interferon by lymphocytes, promote the cell killing effect of natural killer cells, regulate the function of T cells, enhance the chemotaxis and phagocytosis of monocytes, etc. The mechanism of action of the drug shows that the drug is of great significance to liver.
traits
is colorless or light brown clear solution, pH6.5 ~ 7.5.
pharmacological action
For the liver, the main functions of sodium deoxynucleotide are: ①Improve cell enzyme activity and bioelectrical activity, maintain liver cell membrane stability, and promote cysteine glutathione CoA and other important substances in the body Synthesis, improve the detoxification function of the liver, and promote the recovery of liver function; ②Promote the synthesis and mitosis of liver cell DNA, improve the regeneration ability of liver cells, provide metabolic support, maintain the relative stability; ③Direct supplement of nucleotides, mobilize the body to synthesize nucleotides through remedial synthetic pathways, reduce the burden of damaged liver, and help restore liver function. These pharmacological effects of deoxynucleotide sodium on the liver make it widely used in the treatment of hepatitis.
In addition, sodium deoxynucleotide also has the function of regulating the body's immunity. Sodium deoxynucleotide can regulate the function of peripheral lymphocytes, promote the secretion of interleukin 2 and d interferon by lymphocytes, promote the cell killing effect of natural killer cells, regulate the function of T cells, and enhance the chemotaxis and phagocytosis of monocytes. For patients with tumor disease, it has a significant effect on bone marrow suppression and leukopenia caused by other reasons due to the application of anti-cancer drugs or radiotherapy; it has a certain effect on thrombocytopenia and hepatitis.
indications
It is used for the adjuvant treatment of acute and chronic hepatitis, leukopenia, thrombocytopenia and aplastic anemia.
specifications
this product is a compound preparation, and its components are deoxyribonucleotide, deoxyribonucleotide adenine nucleotide, deoxyribonucleotide thymidine nucleotide and deoxyribonucleotide guanine nucleotide sodium salt. 2mL per ampoule, containing 50mg deoxynucleotide, chloride content not exceeding 1%, inorganic phosphorus not exceeding 0.1%. Heavy metals do not exceed 10mg/kg.
usage and dosage
1. intramuscular injection, adult 2~4mL/d, children reduce or follow the doctor's advice.
2. intravenous drip: 50~150mg(1~3 sticks) once, once a day, 30 days as a course of treatment. Add this product to 250ml of 5% glucose injection and slowly drip (2ml per minute).
adverse reactions
occasional transient blood pressure drop
precautions
1. Do not mix with other injections.
2. when it is used for leukopenia after radiation and chemotherapy, blood picture should be monitored regularly during treatment. if the granulocyte count rises to 5000/mm3 (normal value of WBC total number: 4000~10000/mm3) or more during treatment, the condition should be monitored and drug administration should be stopped.
production method
using protamine as raw material
isolate nuclear protein, extract and precipitate frozen protamine 10kg, stir it up, add 100L of water, add sodium chloride to 6%, sodium polycarbonate 1%, stir for 1-2min, add 4 times of water, stir for 1-2min, remove flaky deoxynucleoprotein, add 132L of water, 518kg of 5% SDS solution, stir the solution for 10-20min, centrifuge, take the supernatant, wash with ethanol and acetone and dry to obtain DNA.
protamine [water, sodium chloride] sodium citrate] & rarr; Nuclear protein [water, SDS]& rarr;[10-20min] filtrate [95% ethanol] & rarr;[filtration] DNA
enzymolysis, adsorption, elution 500g of DNA, 10L of distilled water, stir and dissolve, cold storage overnight, dilute the DNA viscous liquid to 50L, heat at 90 ℃ for 10min, denature it, adjust the pH value to 5.4 with hydrochloric acid, add 5ml of citrinin fermentation solution, keep the temperature at 72 ℃ for 1.5h for enzymolysis, adjust the pH value to 7 with ammonia water, heat to 90 ℃, and make the protein denature, precipitation, cold storage overnight, centrifuge, remove the protein, dilute 1 times, adjust pH8.5, 717 or 711OH resin column adsorption, wash with distilled water until the effluent is pH7, elute with 0.2mol/L hydrochloric acid solution, start collection at pH2.5, until pH0.5. The eluent was adjusted to pH7 with 10mol/L sodium hydroxide, and the cold storage was overnight.
DNA [phosphodiesterase] & rarr;[pH5.4, 72 ℃] enzyme fresh liquid [amine hydrochloride] & rarr;[pH7, 90 ℃] filtrate [717 resin] & rarr;[pH8.5] Adsorbent [0.2mol/L hydrochloric acid] & rarr;[pH2.5-0.5] eluent
adsorption, elution, concentration, drying and filtration to remove protein, the filtrate is adjusted to pH 3.5 with hydrochloric acid, the filtrate is 769 activated carbon column, eluted with distilled water twice the volume of the column, and then eluted with ethanol and ammonia (ammonia: 95% ethanol: distilled water = 3:95:47), the eluent is vacuum concentrated to syrup, dehydrated in anhydrous ethanol, ground into powder.
eluent [10mol/L sodium chloride] & rarr;[pH7] filtrate [activated carbon] & rarr;[pH3.5] adsorbate [ethanol, ammonia] & rarr; Elution
the preparation is dissolved in distilled water at 25mg per milliliter, adjusted to pH 6.5-7.5 with 10% sodium hydroxide, added with 0.3% activated carbon, placed for 15min, filtered through diatomite layer, filtered twice with filtrate pulp, added with 0.3% sodium thiosulfate, filtered after dissolution, poured into 2ml ampoule, sealed, sterilized at 100 ℃ for 15min, and mixed deoxynucleotide injection is obtained.
deoxynucleotide [water, sodium hydroxide, activated carbon] & rarr;[pH6.5-7.5, filtered] filtrate [sodium thiosulfate] & rarr; Filtrate [packaging, sealing] & rarr;[sterile] deoxynucleotide injection.