Deoxyribonuclease

Deoxyribonuclease - Names and Identifiers

Name	deoxyribonuclease I F. bovine pancreas
Synonyms	CHRYSENE NEAT Deoxyribonuclease DeoxyribonueleaseⅠ (Dnase I) Deoxyribonuclease I, Bovine Pancreas deoxyribonuclease I F. bovine pancreas Deoxyribonuclease I from bovine pancreas deoxyribonuclease I crudelyophilized powder deoxyribonuclease I type ii-S frombovine pancrea DEOXYRIBONUCLEASE I FROM BOVINEPANCREAS STANDARDIZ Deoxyribonuclease I from Bovine Pancreas(Filtered) Deoxyribonuclease I from bovine pancreas(Recombinant)
CAS	9003-98-9
EINECS	232-667-0

Deoxyribonuclease - Physico-chemical Properties

Molar Mass	38KDa。
Density	100g/mLat 20°C
Water Solubility	125g/L at 25℃
Solubility	Soluble in water, slightly soluble in 30% ethanol and acetone.
Vapor Presure	0.004Pa at 25℃
Appearance	solution (clear, colorless)
Color	white
Merck	13,2923
Storage Condition	-20°C
MDL	MFCD00130918
Physical and Chemical Properties	White-like freeze-dried powder, soluble in water, slightly soluble in 30% ethanol and acetone, the optimum pH value is 7.8. Adding 5mmol/L Ca2 + or diisopropyl fluorophosphate (DFP) to aqueous solution can increase its stability. The inhibitors include EDTA and sodium dodecyl sulfonate; the activator is a divalent metal ion, and the combination of Mg2 and Ca2 can get the best effect. The absorption coefficient of 1% aqueous solution is 11.1 at 280nm. The average strand length that restricts digestion is tetranucleotide, and the enzymatic reaction: deoxyribonucleotide -5 '-phosphate oligonucleotide -5'-phosphate.
Use	For molecular biology research. Various enzymes that degrade DNA. These enzymes hydrolyze the phosphodiester bond on the sugar-phosphate backbone. There are two types of such enzymes: exonuclease and endonuclease.

Deoxyribonuclease - Risk and Safety

Safety Description	S23 - Do not breathe vapour. S24/25 - Avoid contact with skin and eyes. S22 - Do not breathe dust.
WGK Germany	3
RTECS	RF0750000
FLUKA BRAND F CODES	10-21
TSCA	Yes
HS Code	35079090

Deoxyribonuclease - Reference

Reference

1. Yang Xueli, Zhang Honglei, Yang Yutao, etc. Determination of doxorubicin in DNA nano-transport system by HPLC [J]. Journal of Pharmaceutical Analysis 2019(7).
2. Weng Ya Yi Bao Wei Jie Liang Rui Min et al. Effect of MAPK signaling pathway on proliferation, invasion and migration of endometrial carcinoma cells [J]. Journal of Clinical and pathology 2019 000(003):P.470-478.
3. Feng Junye, Li Jiaqi, Wang Ping. Quality changes and volatile compounds analysis of wild Lonicera edulis during fermentation [J]. Modern food science and technology, 2018, 34(11):117-124.
4. Academic Edition) 2018 036(005):82-91 105.
5. Zhou Yue Carnation, Wang yaner et al. Therapeutic effect of ginsenoside Rb1 on Kawasaki disease in mice [J]. Journal of Traditional Chinese Medicine 2017 v.45;No.236(06):40-44.
6. [IF = 3.591] Hengchang Ma et al."Multiple cation-doped linear polymers toward ATP sensing and a cell imaging application."New J Chem. 2018 Jun;42(12):9702-9708
7. [IF=7.086] Weixiang Wang et al."Developmental toxicity and alteration of gene expression in zebrafish embryo exposed to 6-benzylaminopurine."Chemosphere. 2019 Oct;233:336

Deoxyribonuclease - Nature

Open Data Verified Data

This strain of deoxyribose nuclear hydrolase extracted from bovine pancreas is white powder, soluble in water, and Mg2 is activator. The molecular weight was about 31000. It is a highly specific phosphodiesterase, it can cut double-stranded DNA or single-stranded DNA into 5 '-phosphate as the end of the oligonucleotide, the average length of four nucleotides, Mg2 or Mn2, c02, the optimum pH was 7~8. It only acts on extracellular substances and necrotic cells, viscous sputum and contains a large amount of DNA, this product can not only make the sputum DNA rapid lysis, and make the original DNA in sputum combined with protein loss of protection, it is easy to be digested by the proteolytic enzyme in the white blood cells, so that the viscosity decreases rapidly.

Last Update：2024-01-02 23:10:35

Deoxyribonuclease - Use

Open Data Verified Data

expectorant, especially suitable for respiratory infection after the production of a large number of purulent sputum, it not only makes the sputum thinning easily cough, and can make the antibiotic easy to reach the infection foci, give full play to the role of antibiotics. It can also be used locally for abscesses, hematomas, and intrathecal injections to treat meningitis.
usage and dosage spray. 50,000-100,000 units each time, 3-4 times a day. Continuous medication for 4~6 days. Intravenous or intramuscular injection, 1 million units each time. Intrathecal injection, 1 million units each time.
preparation deoxyribonuclease for injection, each containing 25000 units of this product.

Last Update：2022-01-01 11:11:03

Deoxyribonuclease - Safety

Open Data Verified Data

sealed, stored below 4 ℃.

Last Update：2022-01-01 11:11:03

Deoxyribonuclease - Trait

White or off-white lyophilized powder. A double-stranded specific endonuclease that degrades double-stranded DNA into an oligodeoxyribonucleotide having a 5 '-phosphate and a 3'-OH terminus. Single-stranded DNA can also be degraded when a monovalent manganese ion is present.