Detoxargin
L-Arginine hydrochloride
CAS: 1119-34-2;15595-35-4
Molecular Formula: C6H15ClN4O2
Detoxargin - Names and Identifiers
| Name | L-Arginine hydrochloride |
| Synonyms | R-Gene NSC 7914 Argivene Argamine (Levargin AI3-18300 R-Gene 10 Detoxargin NSC 203450 H-Arg-OH·HCl Arginine,(L) Minophagen A L-Arginine HCl UNII-F7LTH1E20Y Einecs 239-674-8 Arginine monochloride Arginine hydrochloride L-Arginine hydrochloride Arginine,hydrochloride,L- L-Arginine Monohydrochloride laevo-arginine hydrochloride L-(+)-Arginine monohydrochloride L-ARGININE HYDROCHLORIDE SOLUTION N~5~-(diaminomethylidene)ornithine N~5~-(diaminomethylidene)-D-ornithine N~5~-(diaminomethylidene)-L-ornithine L-2-amino-5-guanidovaleric acid Hydrochloride 2-Amino-5-guanidinovaleric acid monohydrochloride |
| CAS | 1119-34-2 15595-35-4 |
| EINECS | 239-674-8 |
| InChI | InChI=1/C6H14N4O2.ClH/c7-4(5(11)12)2-1-3-10-6(8)9;/h4H,1-3,7H2,(H,11,12)(H4,8,9,10);1H |
Detoxargin - Physico-chemical Properties
| Molecular Formula | C6H15ClN4O2 |
| Molar Mass | 210.66 |
| Melting Point | 226-230℃ |
| Boling Point | 409.1°C at 760 mmHg |
| Specific Rotation(α) | 22 °(C=8,6N HCL) |
| Flash Point | 201.2°C |
| Solubility | Easily soluble in water (90%,25°C). Slightly soluble in hot ethanol, insoluble in ether. |
| Vapor Presure | 7.7E-08mmHg at 25°C |
| Appearance | White to off-white (Solid) |
| Storage Condition | 2-8°C |
| Sensitive | Sensitive to light |
| MDL | MFCD00064550 |
| Physical and Chemical Properties | is a white crystalline powder, odorless, bitter in taste, soluble in water, acidic in aqueous solution, very slightly soluble in alcohol, insoluble in ether. mp 224 ℃, isoelectric point 10.76, [α]20D 20.4 °-22.4 ° (C = 3 g/mL, 1mol/L HCl) arginine is a semi essential amino acid, which can promote the formation and excretion of urea, correct ammonia poisoning, relieve hepatic coma. In nature, widely exists in a variety of proteins, such as pig hair, hoof nails, blood meal, made of hydrochloride. |
| Use | Used as pharmaceutical raw materials and food additives |
Detoxargin - Risk and Safety
| Safety Description | S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | - |
| RTECS | CF1995500 |
| FLUKA BRAND F CODES | 10 |
Detoxargin - Upstream Downstream Industry
| Raw Materials | Charcoal Hydrochloric acid |
Detoxargin - Reference
| Reference Show more | 1. Yu Xiaomeng, Wang Hechen, Tian Lulu, et al. Preliminary Study on Serum Pharmacochemistry of Qi Zhi Weitong Granules Based on Gastrointestinal Motility Disorder Model Rats [J]. Pharmaceutical Research, 2017, 036(002):P.63-66,74. 2. [IF = 3.398] Hao Zhang et al."α-Glucosidase-Mediated Glucometer Readout for Portable Monitoring of Acarbose and Migliol." Chemosensors. 2022 Jun;10(6):198 |
Detoxargin - Nature
Open Data Verified Data
- This product is white crystalline powder; Odorless; Bitter taste. Soluble in water, aqueous solution acidic reaction. Very slightly dissolved in ethanol. The decomposition temperature is 23 5 deg C.
- This product is the hydrochloride of L-arginine. Arginine is a semi-essential amino acid that is less synthesized in the human body and needs to be partially supplemented from food. In the body it is an intermediate metabolite of ornithine cycle, can promote the production and excretion of urea, correct ammonia poisoning and can relieve hepatic coma. Arginine is the main component of sperm protein, and also has the function of promoting sperm production and providing sperm movement energy. In nature, it widely exists in pig hair, hoof nails, blood meal, gelatin, protamine, small miscellaneous fish. Due to the strong alkalinity of arginine aqueous solution, it absorbs carbon dioxide from the air and is made into hydrochloride.
Last Update：2024-01-02 23:10:35
Detoxargin - Preparation Method
Open Data Verified Data
- hydrolysis method.
- fermentation method.
Last Update：2022-01-01 11:10:14
Detoxargin - Standard
Authoritative Data Verified Data
This product is L-2-amino -5-guanidopentanoic acid hydrochloride. Calculated as dry product, containing C6H14N402 • HC1 should be not less than 98.5%.
Last Update：2024-01-02 23:10:35
Detoxargin - Trait
Authoritative Data Verified Data
- This product is white or off-white crystalline powder.
- This product is soluble in water, very slightly soluble in ethanol.
specific rotation
take this product, precision weighing, add 6mol /L hydrochloric acid solution to dissolve and quantitatively dilute to make a solution containing about 80mg per lml, and determine according to law (General 0621 ), the specific rotation was 21.5 ° to 23.5 °.
Last Update：2022-01-01 15:35:37
Detoxargin - Introduction
Nitric oxide synthase substrate, which can be converted to citrulline and NO. Insulin secretion is induced by a NO-dependent mechanism.
Last Update：2022-10-16 17:12:08
Detoxargin - Use
Open Data Verified Data
- amino acid drugs. It is an important component of amino acid infusion and comprehensive amino acid preparation, and can be used as a nutritional supplement.
- usage and dosage for treatment of hepatic coma, decreasing blood ammonia: 15~20g each time, diluted with 5% glucose looml, and then dropped within 4 hours: arginine equivalent to 48mmol hydrochloric acid per lOg; Treatment of male infertility: Oral 4g daily, three months for a course of treatment.
Last Update：2022-01-01 11:10:15
Detoxargin - Differential diagnosis
Authoritative Data Verified Data
- appropriate amounts of the product and the arginine reference product were dissolved and diluted with water to prepare a solution containing about 0.4mg per 1 ml as the test solution and the reference solution. According to the chromatographic condition test under other amino acids, the position and color of the main spot of the test solution should be the same as that of the reference solution.
- The infrared absorption spectrum of this product should be consistent with that of the control (Spectrum set 406).
Last Update：2022-01-01 15:35:37
Detoxargin - Safety
Open Data Verified Data
- healthy person once intravenous drip of this product 30g or liver insufficiency patients intravenous drip 30g, can be tolerated, no side effects. Intravenous drip too fast can cause salivation, Vomit, facial flushing and so on. Hyperchloremic acidosis can be caused by high-dose infusion. Anuria or renal dysfunction in patients with caution or contraindication.
- closed storage.
Last Update：2022-01-01 11:10:15
Detoxargin - Exam
Authoritative Data Verified Data
transmittance of solution
Take lg of this product, Add 10ml of water to dissolve, according to ultraviolet-visible spectrophotometry (General rule 0401 ), measure the transmittance at 430nm wavelength, not less than 98.0%.
sulfate
take 0.5g of this product and check it according to law (General rule 0802). Compared with the control solution made of 0.02% of standard potassium sulfate solution, it should not be more concentrated ().
phosphate
take 0.4g of this product, place it in the crucible, add 0.3g of magnesium nitrate and 5ml of water, put it in a water bath and evaporate to dryness, burn it with small fire until it is completely ash, add 5ml of water and 3ml of sulfuric acid solution (1- 4), slowly heat for 5 minutes, heat 10ml of water, filter, put the filtrate in a colorimetric tube, and wash the filter residue with an appropriate amount of hot water, add ammonium molybdate solution [take ammonium molybdate 0.5g, add sulfuric acid solution (3-100) 10ml to dissolve] and phosphorus test solution 1ml each, heat at 60°C for 10 minutes, such as blue, with standard phosphate solution (precision weigh 0.143g of potassium dihydrogen phosphate, put in 1000ml measuring flask, add appropriate amount of water to dissolve and dilute to the scale) 0.02% ml of control solution prepared by the same method should not be deeper ().
ammonium salt
take 0.10g of this product and check it according to law (General rule 0808). Compared with the control solution made of 0.02% of standard ammonium chloride solution, it shall not be deeper ().
Protein
Take lg of this product, Add 10ml of water to dissolve, add 5 drops of 20% trichloroacetic acid solution, and no precipitation is allowed.
other amino acids
take an appropriate amount of this product, add water to dissolve and dilute to make a solution containing about 10 mg per lml as a test solution; Take 1ml for precision measurement and put it in a 500ml measuring flask, dilute to scale with water, shake, as a control solution; Take the appropriate amount of arginine and lysine hydrochloride in the same flask, water was added to dissolve and diluted to prepare a solution containing about 0.4mg each per 1ml as a system-suitable solution. According to the thin layer chromatography (General 0502) test, Draw 5 u1 of each of the above three solutions, respectively point on the same silica gel G thin layer plate, with N-propanol-concentrated ammonia solution (2:1) as the developing solvent, expand, dry, spray with ninhydrin in acetone solution (1- 50 ), and heat at 105°C until spots appear, and examine immediately. The control solution should show a clear spot, and the system applicable solution should show two completely separated spots. The test solution should not have more than one impurity spot, and the color should not be deeper (0.2%) than the main spot of the control solution.
loss on drying
take this product, dry at 105°C for 3 hours, loss of weight shall not exceed 0.2% (General rule 0831).
ignition residue
not more than 0.1% (General rule 0841).
Iron Salt
take 2.0g of this product and check it according to law (General rule 0807). Compared with the control solution made of 0.001% of standard iron solution, it should not be deeper ().
Heavy metals
take 2.0g of this product, add 23ml of water and 2ml of acetate buffer (pH 3.5) to dissolve, and check according to law (General rule 0821 first law), containing heavy metals shall not exceed 10 parts per million.
arsenic salt
take 2.0g of this product, add 23ml of water to dissolve, add hydrochloric acid 5mU to check according to law (General rule 0822 first law), should comply with the provisions (0.0001%).
pyrogen
take this product, add Sterile Water for Injection dissolved and diluted to make a solution containing 50mg per lml, check according to law (General rule 1142), dose according to rabbit body weight per lkg injection 10ml, should comply with the regulations. (For injection)
chlorine content
take about 0.35g of this product, precision weighing, add 20ml of water to dissolve, add 2ml of dilute acetic acid and 8~10 drops of bromophenol blue indicator solution, and use silver nitrate titration solution (0.lmol/ L) titrated to blue-purple. Each 1 ml of silver nitrate titration solution (0.1 mol /L) corresponds to 3.545mg of C1. The chlorine content shall be 16.5%-17.1% based on the dry product.
Last Update：2022-01-01 15:35:38
Detoxargin - Content determination
Authoritative Data Verified Data
take this product about O.lg, precision weighing, add anhydrous formic acid 3ml to dissolve, add glacial acetic acid 50ml and Mercury acetate test solution 6ml, according to the potential titration method (General rule 0701), with perchloric acid titration solution (0.1 mol /L) titration, and the results of the titration were corrected with a blank test. Each 1 ml perchloric acid titration solution (0.1 mol /L) corresponds to 10.53mg of C6H14N402. HCl.
Last Update：2022-01-01 15:35:39
Detoxargin - Category
Authoritative Data Verified Data
amino acid drugs.
Last Update：2022-01-01 15:35:39
Detoxargin - Storage
Authoritative Data Verified Data
sealed storage.
Last Update：2022-01-01 15:35:39
Detoxargin - Arginine hydrochloride tablets
Authoritative Data Verified Data
This product contains Arginine hydrochloride (C6H14N402 • HCl) should be labeled the amount of 90.0% to 110.0%.
trait
This product is white or off-white.
identification
- take an appropriate amount of fine powder of this product (equivalent to 0.5g of Arginine hydrochloride), add 5ml of water, stir to dissolve Arginine hydrochloride, filter, take 1ml of filtrate, add naphthol solution (take "-naphthol g, 10% sodium hydroxide solution (0.2ml) and sodium hypobromate solution (0.5ml of bromine and 20ml of 5% sodium hydroxide solution) were added to dissolve, respectively.
- an appropriate amount of the filtrate under identification (1) was taken and diluted with water to prepare a solution containing about 0.4mg of Arginine hydrochloride per 1 ml as a test solution. The same results should be shown in the identification (1) Test under Arginine hydrochloride.
- in the chromatogram recorded under the content determination item, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the reference solution.
- The filtrate test under identification (1) should show the identification reaction of chloride (General rule 0301).
- two items (2) and (3) above can be selected as one item.
examination
should be in accordance with the relevant provisions under The tablet item (General rule 0101).
Content determination
- an appropriate amino acid analysis method or high performance liquid chromatography (General 0512) is used for the measurement.
- chromatographic conditions and system suitability test using eighteen alkyl silane bonded silica gel as filler; With 0.05mol /L acetate buffer (pH 6.4)- 50% acetonitrile solution (65:35) as mobile phase; the detection wavelength was 362nm. The number of theoretical plates shall not be less than 3000 calculated as arginine peak, and the separation degree between arginine peak and internal standard peak shall meet the requirements.
- preparation of internal standard solution an appropriate amount of alanine was taken, dissolved and diluted with water to prepare a solution containing 0.5mg per 1 ml, and then obtained by shaking.
- determination Method: Take 20 tablets of this product, accurately weigh, grind, accurately weigh an appropriate amount (about equivalent to Arginine hydrochloride lOOmg ), put it in a 100ml measuring flask, add water to dissolve and dilute to the scale, 1ml of filtrate and 1ml of internal standard solution were added to the same 10ml measuring flask, and 0.5mol/ L sodium bicarbonate solution (pH 9.0) was added. 1ml with 1% 2, 4-dinitrofluorophenylacetonitrile solution 150 u1, shake, place in a water bath (60°C ± 2°C) in the dark for 60 minutes, remove, cool, dilute to the scale with phosphate buffer (pH 7.0), shake well, as a test solution, accurately take 10ul into the liquid chromatograph, record the chromatogram. An appropriate amount of arginine reference substance was accurately weighed, dissolved with water and quantitatively diluted to prepare a solution containing 0.8mg per 1ml as a reference solution. Take 1ml of each of the reference solution and the internal standard solution for precise measurement, place them in the same 10ml measuring flask, and operate with the same method. The content of Arginine hydrochloride (C6H14N402 • HCl) in the test product was calculated by the peak area according to the internal standard method, and the result was multiplied by 1.209.
category
Same as Arginine hydrochloride.
specification
0.25g
storage
sealed and stored in a dry place.
Last Update：2022-01-01 15:35:40
Detoxargin - Arginine hydrochloride injection
Authoritative Data Verified Data
This product is a sterile aqueous solution of Arginine hydrochloride. The content of Arginine hydrochloride (C6H14N402 • HCl) shall be between 95.0% and 105.0% of the labeled amount.
trait
This product is colorless and clear liquid.
identification
- take this product, add ninhydrin about 2mg, heat, the solution showed blue purple.
- an appropriate amount of this product is diluted with water to prepare a solution containing about 0.4mg of Arginine hydrochloride per 1 ml as a test solution. The same results should be shown in the identification (1) Test under Arginine hydrochloride.
examination
- the pH value should be 3.0 to 5.0 (General 0631).
- pyrogen to take this product, according to the law (General rule 1142), the dose of rabbit body weight per lkg slowly injected 5ml, should comply with the provisions.
- others should comply with the relevant provisions under injection (General 0102).
Content determination
take 10ml of this product, put it in a 100ml measuring flask, dilute it to the scale with hydrochloric acid solution (6-10), shake it well, and determine the optical rotation according to law (General rule 0621 ), the Weight (g) of the test product containing Arginine hydrochloride (C6H14N402. HCl) is obtained by multiplying by 4.444.
category
Same as Arginine hydrochloride.
specification
20ml:5g
storage
sealed storage.
Last Update：2022-01-01 15:35:41
Detoxargin - Reference Information
| Chemical properties | is a white crystalline powder, odorless, bitter and astringent, soluble in water, and acidic in aqueous solution, very slightly soluble in alcohol, insoluble in ether. mp 224 ℃, isoelectric point 10.76, [α]20D 20.4 °-22.4 ° (C = 3 g/mL, 1mol/L HCl) arginine is a semi-essential amino acid, it can promote the formation and excretion of urea, correct ammonia poisoning, relieve hepatic coma. In nature, widely exists in a variety of proteins, such as pig hair, hoof nails, blood meal, made of hydrochloride. |
| Use | there is no side effect in healthy people. But the intravenous drip too fast can cause salivation, Vomit, facial flushing and so on. Hyperchloremic acidosis can be caused by high-dose infusion. Anuria or renal dysfunction in patients with caution or contraindication. used in biochemical research, nutritional medicine. used as pharmaceutical raw materials and food additives |
| production method | gelatin is used as raw material, hydrolyzed by hydrochloric acid, concentrated, neutralized with sodium hydroxide, benzaldehyde condensation to benzylidene arginine crude, followed by hydrochloric acid hydrolysis, acidification, concentration, crystallization of L-Arginine hydrochloride. with gelatin as raw material, after acid hydrolysis, separation and purification. Gelatin [HCl,(hydrolyzed)]→[116-122 ℃, 16h] hydrolysate [reduced pressure] →[(concentrated)] concentrate [NaOH (neutralized)]→[pH 10.5-11] neutralized solution [condensation] →[pH 8] crude benzylidene arginine [HCl (hydrolysis)]→ [boiling] hydrolyzed solution [activated carbon (decolorization)]→ decolorizing solution [303 × 2 resin (adsorption)]→[pH7-8] filtrate [HCl (acidification)]→[pH3-3.5] acidizing solution [concentrated, preparation of crude L-Arginine hydrochloride benzylidene arginine gelatin and 2 times the amount of industrial hydrochloric acid were put into a hydrolysis tank and heated at 116-122 ° C. For reflux for 16h to obtain a hydrolysate. When concentrated to 1/2 volume under reduced pressure, diluted to the original volume with distilled water, and concentrated to obtain concentrated solution. After cooling, slowly add 30% NaOH solution, constantly stirring, and make the temperature below 10 ℃, adjust the pH to 10.5-11, and then slowly drop add benzaldehyde, when the pH is 8, the dropwise addition of benzaldehyde was stopped, the reaction was stirred for 0.5H to complete the reaction, and benzylidene arginine was crystallized and precipitated. After standing for 6h, the crystals were filtered, washed with water, filtered dry, crushed, and dried at 60 ℃, the crude product of benzylidene arginine was obtained. Crude product hydrolysis, separation and purification in the benzylidene arginine crude, adding its amount of 0.8 times of 6mol/L hydrochloric acid, heating and boiling for 50min acid hydrolysis, hydrolysis to 40min, adding a small amount of activated carbon decolorization, after filtration, the residue was washed with hot water and filtered again, the washing solutions were combined and allowed to stand for separation. Separation of the upper layer of benzaldehyde solution to be recovered, the lower aqueous solution, add the treated weak basic styrene type anion resin 303 × 2, adsorption, until pH 7-8 (about 3H), filter off the resin, the filtrate was collected. Add 6mol/L HCl to acidify the collected filtrate, bring the pH to 3-3.5, add appropriate amount of activated carbon, heat and stir for 10min, filter the filtrate, and then keep the temperature at 80-90 ℃ in a water bath and concentrate under reduced pressure, when there is a small amount of crystallization, stop concentrating under reduced pressure, cool the crystals, filter to take the crystals, wash with 70% ethanol, then wash with 95% ethanol, filter dry, dry at 80 ℃, L-Arginine hydrochloride was purified. The overall yield was about 4.5%. |
Last Update：2024-04-09 21:21:28
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View History
Raw Materials for Detoxargin