Name | Ficin froM Fig Tree Latex |
Synonyms | FICIN FICIN,POWDER E.C. 3.4.4.12 FIG TREE LATEX Ficin froM Fig Tree Latex Ficin Agarose from fig tree latex |
CAS | 9001-33-6 |
Water Solubility | Soluble in cold water. |
Appearance | Morphological saline suspension, color tan |
Storage Condition | -20°C |
MDL | MFCD00081597 |
Physical and Chemical Properties | Chemical white to yellowish or cream powder. The molecular weight is about 26000, the isoelectric point is 9.0, and the pH value of 2% aqueous solution is 4.1. The stability is extremely great. For example, if the normal temperature is sealed and preserved for 1~3 years, its effectiveness will only decrease by 10% ~ 20%. The aqueous solution is inactivated at 100°C, while the powder is inactivated at 100°C for several hours. The activity of aqueous solution is stable at pH 4~8.5. Its activity can be inhibited by iron, copper, and lead. Its aqueous solution can hydrolyze gelatin, coagulated protein, cheese cord, meat, liver, etc., solidify the emulsion, and digest parasite eggs such as roundworms and whipworms. There is a certain hygroscopicity. Can be completely dissolved in water, light brown to dark brown. Insoluble in general organic solvents. If it is a concentrated preparation of fig glue emulsion, it is light brown to dark brown. The main principle of action is to hydrolyze polypeptides into low molecular peptides. The optimum pH = 5.7; The optimum temperature is 65 ℃. |
Use | Use enzyme preparation. It is mainly used for cold resistance of beer (hydrolyzing protein in beer to avoid turbidity caused by cold storage); Softening of meat (hydrolyzing muscle protein and collagen to tender meat); Dough conditioner when baking; Emulsion coagulant when making cheese (instead of rennet enzyme). Separate the shell of the shrimp from the meat to achieve mechanized shelling, and also separate the clam meat from the internal organs. |
Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R42 - May cause sensitization by inhalation |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S36/37 - Wear suitable protective clothing and gloves. S24 - Avoid contact with skin. S22 - Do not breathe dust. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S23 - Do not breathe vapour. |
WGK Germany | 3 |
RTECS | LK3675000 |
TSCA | Yes |
Toxicity | LD50 orally in rats, mice: ~10 g/kg; in rabbits, guinea pigs: ~5 g/kg (Molitor) |
Merck | 14,4077 |
EPA chemical information | Ficin (9001-33-6) |
Toxicity
ADI postponed the decision (FA0/wH0,2001).
LD5010g/k (rat, oral).
The enzyme activity determination method of fig protease can use a general plant protease activity determination method. See "Method 1" in "Papain (16020)".
Production method
the latex of fig (Ficus sp.) tree and immature fruit milk are extracted with water with pH 4.0, salted out (secondary) with ammonium sulfate in the presence of ethylenediaminetetraacetic acid (EDTA), and then salted out twice with salt. The yield is about 11%.
a green fig weighing 10~15g can contain 100~150mg commercial fig protease. After being dried by sunlight, only about 12% of vitality remains, and fruits dried at 100 ℃ have no activity.