FluoresceinPEGNHS,FITC-PEG-NHS - Names and Identifiers
FluoresceinPEGNHS,FITC-PEG-NHS - Physico-chemical Properties
FluoresceinPEGNHS,FITC-PEG-NHS - Introduction
FluoresceinPEGNHS,FITC-PEG-NHS(FluoresceinPEGNHS,FITC-PEG-NHS for short) is a fluorescent probe molecule. It consists of fluorescein and polyethylene glycol (PEG) linked via N-hydroxysuccinimide (NHS). FITC stands for fluorescent fluorescein isothiocyanate, and NHS is an active ester compound used to react with amines to form ester bonds.
Nature:
-Appearance: FITC-PEG-NHS yellow-green solid
-Solubility: FITC-PEG-NHS can be dissolved in various organic solvents such as DMF, DMSO and methanol
-Fluorescence: The FITC part makes it have a strong fluorescent signal, suitable for fluorescent staining and related experiments
Use:
-Biomarkers: FITC-PEG-NHS cell markers, protein markers, etc. commonly used in biological research
-Fluorescence imaging: Because the FITC-PEG-NHS has a strong fluorescence signal, it can be used for fluorescence microscopy of cells and tissues
-Fluorescent probes: FITC-PEG-NHS can be used to detect various biomolecules and targets, such as proteins, antibodies, nucleic acids, etc.
Preparation Method:
A common method for preparing FITC-PEG-NHS is to react fluorescein with polyethylene glycol-N-hydroxysuccinimide under alkaline conditions. The usual experimental conditions are the reaction of fluorescein with polyethylene glycol-NH2 and NHS in alkaline medium to produce FITC-PEG-NHS.
Safety Information:
- FITC-PEG-NHS can cause irritation and contact with eyes, skin and mucous membranes should be avoided
-Use with appropriate protective equipment such as gloves, goggles and lab coat
-should be operated in a well-ventilated laboratory
-Avoid inhalation or ingestion
-In case of accidental contact, rinse immediately with plenty of water and seek medical help
-Detailed safety information should be found in the safety data sheet (SDS) provided by the product supplier
Last Update:2024-04-09 21:04:16