Physical and Chemical Properties | Chemical properties dark blue powder. Easily soluble in hydrophilic solvents such as water, aqueous ethanol and aqueous propylene glycol, it is bright blue. Insoluble in organic solvents. Almost odorless, tasteless. The moisture absorption is small. The hue does not change within the pH value of 4~8, and does not fade after heating at 120 ℃ for 60min. Absorbance (1000 times diluted aqueous solution) 0.5(590nm). Poor light resistance. It is almost unaffected by Ca2, Mg2, Al2, etc., but it becomes deeper when it is acidic. The staining for protein is stronger than that for starch. |
Use | It is suitable for the coloring of candy, beverage, cake and other foods, and can also be used as the coloring of medicine, cosmetics and other health products. |
overview
Gardenia blue is an internationally popular natural food additive with strong water solubility. It is a dark blue powder made from gardenia fruit through microbial fermentation or enzyme biotransformation. Gardenia blue pigment has the advantages of stable physical and chemical properties, good tinting power and food safety because of its unique blue tone. Since 1989, China has listed it as a licensed food additive and is widely used in food and beverage, medicine, daily chemical industry and other fields.
traits
blue powder, almost odorless and tasteless. Easily soluble in water, aqueous ethanol and aqueous propylene glycol, bright blue. There is no change in hue in the range of pH3 ~ 8. Heat resistance, does not fade after 120 ℃ for 60min. Weak moisture absorption and poor light resistance. This product has strong dyeing power for protein and 0.5 absorbance (1000 times diluted aqueous solution, 590nm).
Preparation
it is based on geniposide (geniposide), a chemical component contained in Rubiaceae plant-gardenia, and uses β-1, 4-glucoside enzyme (EC3.2.1.21, betaglucosedase) to hydrolyze geniposide, remove the glucose group at the end of its molecule, and obtain geniposide (genipin). Geniposide undergoes a series of polymerization and rearrangement reactions with amino acids, and gardenia blue can be. Cellulase & rarr; Enzyme solution & rarr; Enzymatic hydrolysis of geniposide & rarr; Genipin & rarr; Amino acid polymerization & rarr; Gardenia blue pigment solution & rarr; Ultrafiltration (UF)& rarr; Freeze drying & rarr; Gardenia blue pigment powder.
use
mainly used as food pigment, suitable for candy, beverage, jam, etc. It can also be used in conjunction with other natural pigments. It is safe and can be added to cosmetics.
Natural sources of natural blue pigments There are few natural blue pigments and very limited natural sources. Inorganic natural blue pigment mainly comes from natural blue alum ore, and now a large part is synthesized by chemical methods. Organic natural blue pigments are mainly derived from plant and microbial materials. The leaves of wood blue plants such as Polygonum, Tea Blue, Horse Blue, Wu Blue, Isatis can be used to make indigo dyes. Gardenia blue pigment is prepared with geniposide from the fruit of the Rubiaceae plant Gardenia. The fruits of Ophiopogon japonicus, Acanthaceae plants, mountain blue, purple cabbage, blue wheat seeds and other plant-derived materials are all used to prepare the corresponding blue pigments. Although genetically modified cotton and genetically modified roses that can produce blue pigments have also been reported, such genetically modified plant materials cannot be used to produce natural blue pigments due to their rare sources. Spirulina, cyanobacteria, nostoc and other algae can be used to produce phycocyanin pigment. Garlic can turn green after being soaked in vinegar, and blue pigment can be separated. Some microorganisms such as Streptomyces, Pseudomonas, Pseudomonas, Dullobacterium, Aureobasidium pullulans, purple non-sulfur bacteria, etc. can use the culture medium to produce different blue pigments during the growth process. There are also some genetically engineered strains that can produce indigo pigment. Although natural blue pigments are similar in blue tones, the color mechanism of blue pigments is not the same. The different coloration mechanisms are ultimately determined by the chemical structure or spatial structure of the pigment molecule. The molecular structure of natural blue pigments prepared by different raw materials or different methods may be different, and the physical and chemical properties and application scope of the pigments will also be different.
use
coloring of general food, such as hard candy, pectin, agar and other gel fudge, pudding, mahimaluo sugar, biscuits, pine cake, cake pre-made powder, cream, ice cream, dairy products, vegetables, green beans and other cans, beverages, fruit juice and so on.
production method
from Rubiaceae plant gardenia (Gardenia augustavar. gnrdiftoru;G .. florida and G.in.sminoides) fruits are extracted with warm water and a mixture of color glycosides and protein decomposition products (natural amino acids should be used, and the finished product is acid-resistant, if soybean protein is used, it is not acid-resistant), add β-galactosidase (made from koji bacteria) through fermentation, inactivation, filtration, evaporation, refining, and drying. It is blue.
Aspergillus niger can also be used as an enzyme-producing strain. under the optimized conditions, the enzyme activity increased from the original 51.5U/ml to 96.5U./ml. The optimum culture conditions are: bran 4%, ammonium sulfate 0.1%, potassium dihydrogen phosphate 0.2%, ascorbic acid 0.1%, sodium acetate 0.1%, magnesium sulfate 0.02%, ferric sulfate 0.001%, initial pH value 7.0, inoculation amount 10%, culture conditions 30 ℃,150r /min,72h.