H-Lys-Arg-Arg-Glu-Ile-Leu-Ser-Arg-Arg-Pro-Ser-Tyr-Arg - Names and Identifiers
H-Lys-Arg-Arg-Glu-Ile-Leu-Ser-Arg-Arg-Pro-Ser-Tyr-Arg - Physico-chemical Properties
Molecular Formula | C73H129N29O19
|
Molar Mass | 1716.99 |
Solubility | H2O: soluble |
Appearance | lyophilized powder |
Storage Condition | −20°C |
H-Lys-Arg-Arg-Glu-Ile-Leu-Ser-Arg-Arg-Pro-Ser-Tyr-Arg - Risk and Safety
Safety Description | S22 - Do not breathe dust.
S24/25 - Avoid contact with skin and eyes.
|
WGK Germany | 3 |
H-Lys-Arg-Arg-Glu-Ile-Leu-Ser-Arg-Arg-Pro-Ser-Tyr-Arg - Introduction
Green Fluorescent Protein (GFP) is a naturally occurring protein with fluorescent properties. It can be excited by a specific excitation light wavelength (usually ultraviolet) and fluoresces green.
GFP has the following important properties:
1. Fluorescence characteristics: GFP spontaneously produces fluorescence without exogenous dyes, and the absorption and emission wavelengths of light are 395 nm and 509 nm, respectively.
2. Polymorphism: GFP can be combined in different tissues and cells, and has a wide range of applications.
3. Stability: GFP is relatively stable, not easy to inactivate or decompose, and has a long half-life.
4. High brightness: The fluorescence signal of GFP is strong and can be clearly observed by the detector.
The application of GFP is very extensive:
1. biological fluorescent markers: by fusing GFP with other proteins or chromosomes, the visualization of specific components in cells, tissues and organisms can be realized.
2. Gene expression analysis: By introducing the GFP gene, changes in gene expression at different developmental stages or environments can be studied.
3. Protein localization research: Using GFP to label proteins can study the localization and movement of proteins in cells.
The preparation of GFP usually uses genetic engineering technology, that is, the GFP gene is inserted into the target organism or cell, so that it is expressed in the organism. The gene of GFP has been widely cloned and can be obtained by DNA recombination or artificial synthesis.
Note the following safety information when using GFP:
1. GFP is generally considered to be a relatively safe protein, but in the process of experimental operation, it is still necessary to comply with the relevant biosafety operating specifications.
2. in the genetic engineering operation, it is necessary to comply with the corresponding technical guidance and laws and regulations, and ensure the correct disposal of waste.
3. When using GFP in the laboratory, pay attention to avoid direct contact and intake, and use personal protective equipment reasonably, such as laboratory gloves and laboratory glasses.
4. When using a fluorescence microscope to observe GFP, avoid long-term continuous light to reduce negative effects on biological activity.
Last Update:2024-04-09 19:05:35