Hepes,LowSodium,FreeAcid
4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid
CAS: 7365-45-9
Molecular Formula: C8H18N2O4S
Hepes,LowSodium,FreeAcid - Names and Identifiers
Hepes,LowSodium,FreeAcid - Physico-chemical Properties
| Molecular Formula | C8H18N2O4S |
| Molar Mass | 238.3 |
| Density | 1.07g/mLat 20°C |
| Melting Point | 234-238 °C |
| Boling Point | 408℃[at 101 325 Pa] |
| Water Solubility | Soluble |
| Solubility | Soluble in water (2.25mol/L,0 ℃) |
| Vapor Presure | 0Pa at 25℃ |
| Appearance | White crystalline powder |
| Color | White |
| Maximum wavelength(λmax) | ['λ: 260 nm Amax: 0.06', , 'λ: 280 nm Amax: 0.04'] |
| Merck | 14,4654 |
| BRN | 883043 |
| pKa | 7.5(at 25℃) |
| PH | 5.0-6.5 (1Maqueous solution) |
| Storage Condition | 2-8°C |
| Stability | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
| Refractive Index | n20/D 1.339 |
| MDL | MFCD00006158 |
| Use | Used as a Biochemical reagent |
Hepes,LowSodium,FreeAcid - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S24/25 - Avoid contact with skin and eyes. S22 - Do not breathe dust. S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| WGK Germany | 1 |
| RTECS | TL6809000 |
| TSCA | Yes |
| HS Code | 29335995 |
Hepes,LowSodium,FreeAcid - Introduction
N-(2-hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid) is one of the best buffers for all biological studies. HEPES is a zwitterionic organic chemical buffer under most physiological PHS, which is one of Good's buffer reagents and has the strongest buffer capacity in the range of pH 6.0-8.5. HEPES is widely used, including: 1) as a buffer system for cell culture, the common working concentration is 10-25 mM; Compared with inorganic bicarbonate buffer system, HEPES shows better pH control ability in tissue or organ culture. Unlike bicarbonate, HEPES does not supply nutrients to cells. Only when the cells need to be treated in the CO2 incubator for a long time will the culture medium be added to enhance the buffering capacity. 2) Used as an amphoteric electrolyte solution; HEPES can dissolve amphoteric electrolytes and prepare a pH gradient of <1 pH unit width for isoelectric focusing electrophoresis experiments; 3) It is used as a suitable buffer system for electron microscope observation of protein deposition technology, because HEPES will not cause negative effects on metal substrates; 4) It can be used as a buffer reagent for quantitative and selective determination of antigen-antibody reaction.
Last Update：2022-10-16 17:28:14
Hepes,LowSodium,FreeAcid - Reference Information
| pH range of acid-base indicator discoloration | 7.0 - 7.6 |
| LogP | -3.85 at 20℃ |
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Introduction | 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) is an important hydrogen ion buffer, it has good buffer capacity in the range of pH 6.8~8.2, and can control the constant pH for a long time. The concentration of 10~50mmol/L, the general culture solution containing 20mmol/L4-Hydroxyethyl piperazine ethanesulfonic acid can have a good buffer capacity, and no toxic effect on cells. |
| Usage | (1)hepes buffer can be directly added to the prepared culture medium at the required concentration, then filter and sterilize. Add 2.38g HEPES to every 7.2 of culture solution, and adjust pH to with lN NaOH after dissolution, filter and use after sterilization. HEPES was used at a concentration of 10 mmol/L.(2) a 100 x stock solution (l mol/L) can also be prepared, and 99mL of culture solution is added to 1 ml of stock solution before use, and the final application concentration is still 10 mmol/L. l mol/L(100 x)hepes buffer preparation method: take 23.8g HEPES dissolved in 90ml Double distilled water, with lN NaOH to pH 7.5 a 8.0, then, the solution was adjusted to 100ml with water, filtered and sterilized, divided into vials (2mL/bottle), and stored at 4 °c or -20 °c. |
| Application | HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) is a non-ionic Amphoteric buffer with high polarity, it is inert to a variety of chemical reagents and enzymes, does not participate in and does not interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions, so it can be specially used for organelles and easily denatured, work on pH-sensitive proteins and enzymes. |
| preparation method | directly use 1, 2-dichloroethane as solvent, with mechanical stirring, add hydroxyethylpiperazine (5.00g,0.02mol), potassium carbonate K2CO3(6.00g,0.04mol),50ml 1, 2-dichloroethane, the mixture was heated in an oil bath at 90 ° C. (boiling point of 1, 2-dichloroethane: 85 ° C.) and stirred for 20H. The reaction was stopped, filtered and the filtered salt was washed with 200ml of ethyl acetate (EA). The filtrate was spin-dried to give 2.6g of Hepes (4-hydroxyethylpiperazine ethanesulfonic acid) as a solid. |
| purpose | used as Biochemical reagent biological buffer; Reaction buffer for separation and analysis of RNA nuclear components, pre-hybridization buffer, hybridization buffer; For RNA and T4RNA Molecular biology grade for RNA 3 '-end labeling with T4 RNA ligase, separation and analysis of nuclear RNA reaction buffer components, pre-hybridization buffer relaxation hybridization buffer composition cell-cell adhesion, short-term cell aggregation culture, buffer used to wash tissues and cells |
Last Update：2024-04-10 22:29:15
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