Hyaluronidase
Hyaluronidase
CAS: 9001-54-1;37326-33-3
Molecular Formula: C18H14O7
Hyaluronidase - Names and Identifiers
Hyaluronidase - Physico-chemical Properties
| Molecular Formula | C18H14O7 |
| Molar Mass | 342.29956 |
| Appearance | Morphological lyophilized powder |
| Color | white to slightly brown |
| Merck | 13,4777 |
| Storage Condition | −20°C |
| Physical and Chemical Properties | White or light yellow flocculent freeze-dried, odorless, soluble in water, insoluble in ethanol, acetone and ether, the optimum pH value of 4.5-6.0. Stability: The freeze-dried material stored at 4 ℃ for one year showed no significant decrease in vitality; Under the condition of 42 ℃, the activity was unchanged by heating for 60min; 100 ℃ for 5min to retain 80% of the vitality; Low concentration aqueous solution is easy to be inactivated, the addition of NaCl can increase its stability; It is easy to deteriorate when exposed to heat. Inhibitors of heavy metal ions (Cu2 +, HR<2 +, Fe<3 +, Mn<2 +, Zn<2 +), acidic organic dyes, bile salts, polyanions and high molecular weight polysaccharides such as chondroitin sulfate B, heparin, heparan sulfate; Activators are polycations. The absorption coefficient of 1% aqueous solution at 280nm was 8. Hyaluronidase is mainly the hydrolysis of hyaluronic acid in N-acetyl-beta-D-glucosamine and D-glucuronic acid between the beta -1,4-bond, resulting in four sugar residues, do not release monosaccharides, enzyme reaction: hyaluronic acid + H2O oligo-oligosaccharides. |
| Use | It is used in biochemical research, and is commonly used in clinical practice to promote the dissipation of local edema or hematoma after surgery and trauma, promote the infiltration of local anesthetics, reduce the pain at the injection site, and accelerate the absorption of subcutaneous and intramuscular injection, can also be used for intestinal adhesions. |
Hyaluronidase - Risk and Safety
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 3-10 |
| HS Code | 35079090 |
Hyaluronidase - Upstream Downstream Industry
| Raw Materials | Phthalazine terylene Hydralazine |
Hyaluronidase - Reference
| Reference Show more | 1. Chen, Qiaoling, yuan, Lin, Wang, et al. Optimization of extraction process and in vitro anti-inflammatory activity of crude polysaccharides from Atractylodes lancea [J]. Biomedical Engineering and clinical practice 2019(5):517-523. 2. Liu Chang, Chen Yuxin, Wang Jiangfan, et al. Dendritic polypeptide-based multi-functional drug delivery system for precise targeting therapy of tumor cell nuclei [J]. Chinese Journal of Polymer Science, 2018, 000(006):682-691. 3. Zhang Hao, He Linyan, Wang Yan, et al. Study on the efficacy of aloe vera gel in post-Sun repair [J]. Fragrance and flavor cosmetics, 2019, No.174(03):65-71. 4. Qian lunar January, Li Jun, Huang Cheng, etc. Therapeutic effects of different combinations of Zhizi Baipi decoction on hepatic fibrosis induced by carbon tetrachloride in mice [J]. Journal of Anhui Medical University, 2016, 51(001):68-72. 5. Zhang Beibei, Wang Ying, Zhang Ji, et al. Prokaryotic expression of zona pellucida 3 protein and preparation of its antiserum [J]. Sichuan Journal of animals, 2016, 35(005):697-702. 6. Fan Ziyan, Zhao Ziyan, Zhao Xiang, etc. Purification and in vitro anti-inflammatory activity of total triterpenoids from bilberry [J]. Journal of Nanjing Forestry University: Natural Sciences, 2019, 043(004):132-138. 7. Guan Lingjun, Chen Xiaojun, Shen Dianjing, etc. Study on the preparation and moisturizing properties of Anoplophora glabrifolia cream [J]. Journal of Forestry and Environmental Sciences, 2020, 036(002):P.26-32. 8. Sixing Hui. Study on stimuli-responsive polymer materials for controlled release of protein drug delivery [D]. University of Science and Technology of China, 2020. 9. Zhao Yue. Design, preparation and biological application of biomimetic Intelligent Polymer hydrogel materials [D]. Jilin University, 2020. 10. Ma, Xuebin, et al. "In situ photocrosslinked hyaluronic acid and poly (gamma-glutamine acid) hydrogels as insoluble drug carriers for load-bearing tissue application." Journal of Biomaterials Science, Polymer Edition 29.18 (2018): 2252-2266.https:// doi.org/10. 11. [IF=3.517] Xuebin Ma et al."In situ photocrosslinked hyaluronic acid and poly (γ-glutamic acid) hydrogels as injectable drug carriers for load-bearing tissue application."J Biomat Sci-Polym E. 2018;29(18):2252-2266 12. [IF=11.622] Zheng Chunli et al."Systems pharmacology: a combination strategy for improving efficacy of PD-1/PD-L1 blockade."Brief Bioinform. 2021 Sep;22(5): 13. [IF=10.317] Xuemin Liu et al."Molecular recognition-directed site-specific release of stem cell differentiation inducers for enhanced joint repair."Biomaterials. 2020 Feb;232:119644 14. [IF=8.947] Shikun Wei et al."A composite hydrogel with co-delivery of antimicrobial peptides and platelet-rich plasma to enhance healing of infected wounds in diabetes."Acta Biomater. 2021 Apr;124:205 15. [IF=6.529] Yue Xiao et al."Predicting the herbal medicine triggering innate anti-tumor immunity from a system pharmacology perspective."Biomed Pharmacother. 2021 Nov;143:112105 16. [IF=6.205] Haixia Sun et al."Design and Discovery of Natural Cyclopeptide Skeleton Based Programmed Death Ligand 1 Inhibitor as Immune Modulator for Cancer Therapy."J Med Chem. 2020;63(19):11286-11301 17. [IF=5.722] Zhu Jinglin et al."Licorice extract inhibits growth of non-small cell lung cancer by down-regulating CDK4-Cyclin D1 complex and increasing CD8 T cell infiltration."Cancer Cell Int. 2021 Dec;21(1):1-18 18. [IF=3.644] Wang Xinlu et al."Preparation of Antimicrobial Hyaluronic Acid/Quaternized Chitosan Hydrogels for the Promotion of Seawater-Immersion Wound Healing."Front Bioeng Biotech. 2019 Dec;0:360 19. [IF=3.361] Yu Xue et al."Synthesis of hyaluronic acid hydrogels by crosslinking the mixture of high-molecular-weight hyaluronic acid and low-molecular-weight hyaluronic acid with 1,4-butanediol diglycidyl ether."Rsc Adv. 2020 Feb;10(12):7206-7213 20. [IF=2.967] Zihang Chen et al."Sarcoma‑180 tumor affects the quality of oocytes in mice."Oncol Lett. 2021 Mar;21(3):1-1 21. [IF=5.81] Xing Feng et al."Docetaxel-Loaded Novel Nano-Platform for Synergistic Therapy of Non-Small Cell Lung Cancer.."Front Pharmacol. 2022 Mar;13:832725-832725 22. [IF=5.268] Mi Wang et al."Synergistic H2O2 Self-supplying and NIR-Responsive Drug Delivery Nanoplatform for Chemodynamic-Photothermal-Chemotherapy."Colloid Surface B. 2022 Feb;:112412 23. [IF=7.711] Mei Zhao et al."Viscosity-Based Flow Sensor on Paper for Quantitative and Label-Free Detection of α-Amylase and Its Inhibitor."Acs Sensors. 2022;XXXX(XXX):XXX-XXX 24. [IF=11.556] Dong Peng et al."Extracellular vesicles derived from astrocyte-treated with haFGF14-154 attenuate Alzheimer phenotype in AD mice."Theranostics. 2022; 12(8): 3862-3881 |
Hyaluronidase - Nature
Open Data Verified Data
an enzyme that hydrolyzes hyaluronic acid mucopolysaccharides extracted from the mammalian testes of this strain; A basic protein that is a glycosidase, it can catalyze the hydrolysis of hexose glycosidic bonds of some other acidic mucopolysaccharides of hyaluronic acid and connective tissue, such as osteins A and C, and the products are mainly
Even-numbered oligosaccharides such as tetrasaccharides and hexasaccharides. White or yellowish powder; Odorless. Soluble in water, insoluble in ethanol, acetone or ether. Good stability. 42 C heating 60 min activity does not lose, 100 C heating 5min activity can be retained 80%. It is easy to be inactivated in low concentration aqueous solution and can be protected by gum arabic or gelatin. The enzyme was reversibly inhibited by FeZ + and Cu2 + ions. Pbz +, Hgz +, GJZ + plasma had no significant effect on enzyme activity. Chondroitin sulfate B (dermatan), heparan sulfate, keratan sulfate, heparin, and high concentrations of hyaluronic acid all have inhibitory effects on the enzyme, but can be inhibited by 0. 15mol. Reversed By L-l sodium chloride or protamine sulfate. This product can hydrolyze the main component of the tissue matrix is hyaluronic acid, so as to reduce the tissue mucus, improve the permeability of capillaries and tissues, and accelerate the diffusion of substances inside and outside the cell. Clinically, it is beneficial to accelerate the absorption of subcutaneous and intramuscular injection liquid, and is beneficial to sperm entering the egg during fertilization.
Last Update：2024-01-02 23:10:35
Hyaluronidase - Preparation Method
Open Data Verified Data
- extraction of bovine and sheep testis. The frozen testes of sheep and cattle were cut open with a knife, the internal and external cortex and the accessory testis were removed, and minced into a slurry. Weigh the slurry lOokg into the pre-prepared cold acetic acid solution (90kg of water, 600mL of glacial acetic acid, 10L of 1mol/L hydrochloric acid mixed), and stir vigorously at -5 ℃ for 3~4min, after stirring every 5min once, extraction 4H. Then, filtration was performed to obtain 125 to 140L of the extract. The pulp residue was extracted for the second time, and the amount of acid extracted was 1/3 of the first amount, and the extract was combined. With constant stirring, 210g of ammonium sulfate was added to each liter of the extract solution, which was completely dissolved. After the solution was completely dissolved, it was left to stand at about th, and the filtrate was suspended and filtered overnight with polyester cloth to obtain 110L of the filtrate. Then, 290g of ammonium sulfate per liter of the filtrate was added to completely dissolve the filtrate, and the solution was allowed to stand still and filtered overnight to obtain crude product of the enzyme. The crude product was dissolved in 5L of cold distilled water, and 12 5g of ammonium sulfate was added with stirring to completely dissolve the mixture, and the mixture was allowed to stand at 10 ° C. Overnight. The surface fat was removed, the supernatant was siphoned off, and filtered. 750g of ammonium sulfate was slowly added to the filtrate under constant stirring to completely dissolve the filtrate, and the solution was left to stand at about 10 ° C. Overnight. The next day, the supernatant liquid was removed to obtain salting-out material. The salting-out material was dissolved in 500ml of cold distilled water, packed into a dialysis bag, and placed in 25L of phosphate-citric acid buffer solution (citric acid 7. 5G, Na2 hp4.12h20 60g, sodium chloride 70g, made up into 2500mL with water, adjusted to pH 6.5 with citric acid), dialyzed at about 10 ° C. For 24h, and then filtered to obtain dialysate. The dialysate was cooled in an ice bath, 50ml of 15% Na2 HP04 · l2H2 0 solution was added, and 30ml of 20%-water calcium acetate solution was slowly added under constant stirring, and 0.5mol/L sodium hydroxide solution to pH 8.5, continue to stir 10 min, vacuum filtration, filtrate to 0. 5mol/L hydrochloric acid to adjust pH7.O, freeze drying, no pyrogen glass enzyme quality. The yield was more than 2 x 104 units per kg of testis.
- microbial fermentation method.
Last Update：2022-01-01 11:11:14
Hyaluronidase - Standard
Authoritative Data Verified Data
This strain is an enzyme extracted from mammalian testes that can hydrolyze hyaluronic acid mucopolysaccharides. The activity of the hyaluronidase should not be less than 300 units per 1 mg.
Last Update：2024-01-02 23:10:35
Hyaluronidase - Trait
Authoritative Data Verified Data
- This product is white to yellowish powder; Odorless.
- This product is soluble in water and insoluble in ethanol, acetone or ether.
Last Update：2022-01-01 13:38:24
Hyaluronidase - Use
Open Data Verified Data
- proteolytic enzyme. It can accelerate the absorption of subcutaneous and intramuscular injection, promote the infiltration of local anesthetic, reduce the pain of injection site, and promote the dissipation of local edema or hematoma after surgery and trauma. Can also be used for intestinal adhesions.
- usage and dosage: subcutaneous or intramuscular injection, 150~15 00 units/time, 1 time/1~2 days, using normal saline or water for injection to dissolve, then mixed with other injections; Subcutaneous infusion, looml infusion plus 150 units of this product.
- formulation Hyaluronidase for Injection.
Last Update：2022-01-01 11:11:15
Hyaluronidase - Differential diagnosis
Authoritative Data Verified Data
- take this product, add phosphate buffer (General 1207) to dissolve and dilute to make a solution containing 500-1000 units per lml; Take 2 tubes, each add the above solution (1 ml), take one of them to heat and boil, let it cool, then add 1 ml of potassium hyaluronate stock solution (General rule 1207) to each of the two, shake well, place it in 37°C water bath for 30 minutes, and take it out, add 1 ml of serum solution (General rule 1207), shake well, and the solution in the tube without heating and boiling should be clear.
- one healthy guinea pig was injected intradermally with 0.25% methylene blue sodium chloride injection (0. LML) in two places on the back, as a control, the other two intradermal injection solutions containing 10 units of the product in each lml of the solution made of 0.lml, the injection sites should be cross-arranged, the distance between each other should be greater than 3cm, 5 minutes after injection, the animals were sacrificed, the skin was peeled, and the diffusion of methylene blue was observed from the reverse side. The blue circle caused by the test solution should be larger than that caused by the control.
Last Update：2022-01-01 13:38:25
Hyaluronidase - Safety
Open Data Verified Data
- mice injected subcutaneously with LD50> 250,000 units/kg; Mice injected intramuscularly with LD50> 250,000 units/kg. It is contraindicated at the site of infection to prevent spread; Intravenous injection is not allowed; Now it is ready for use. Sometimes there may be allergic reactions, skin test before use. Salicylic acid can inhibit the role of this product.
- sealed, stored in a cool and dry place.
Last Update：2022-01-01 11:11:15
Hyaluronidase - Exam
Authoritative Data Verified Data
pH
take an appropriate amount of this product, add water to dissolve and dilute the solution containing 3mg per lml, and determine it according to law (General 0631). The pH value should be 4.5~7.5.
clarity and color of solution
take this product O.lg, after adding 10ml of water to dissolve, check according to law (General rule 0901 first method and general rule 0902 first method), the solution should be clear and colorless; If the color is colored, it should not be deeper than the yellow No. 4 standard colorimetric solution.
absorbance
take an appropriate amount of this product, accurately weigh, add water to dissolve and quantitatively dilute to make a solution containing 300 units per lml, and measure by UV-visible spectrophotometry (General rule 0401), the absorbance at a wavelength of 280nm shall not be greater than 0.6; The absorbance at a wavelength of 260nm shall not be greater than 0.42.
tyrosine
take two centrifuge tubes A and B, add 1 ml of test solution (containing 5mg of glass enzyme per 1 ml) to the tube A, add 1 ml of water to the tube B, and evaporate to dryness at 105°C, respectively, add 0.2ml of 6mol/L sodium hydroxide solution each, heat with saturated steam at 121 ° C. For 3 hours or in a water bath for 4-5 hours, take it out and let it cool, add 3.5 ml of 0.3 mol/L sulfuric acid solution, 1.5ml of water in a tube, and 0.2ml of tyrosine control solution in B tube (containing 30ug of mol/L sulfuric acid solution of tyrosine control in each lml). 1.5ml, then add 15% ml of 2.5mol/L sulfuric acid solution containing 1.5 mercuric sulfate, heat it in a water bath for 10 minutes, and let it cool to room temperature, add 1 ml of 3.5mol/L sulfuric acid solution and 1 ml of 0.2% sodium nitrite solution, shake, immediately add water to 6ml, time, shake, centrifuge. After 20 minutes, absorb the supernatant, according to UV-visible spectrophotometry (General rule 0401), measure the absorbance at the wavelength of 540mn, each unit containing tyrosine should not exceed 0.1ug.
abnormal toxicity
5 healthy mice weighing 17-22G were injected subcutaneously with 10000 ml of sodium chloride injection containing 0.25 units of hyaluronidase per 1 ml, subcutaneous tissue necrosis or death should not occur within 48 hours. If there is tissue necrosis or death in one mouse, it should be re-tested according to the above method, all mice were free of tissue necrosis or death within 48 hours.
bacterial endotoxin
take this product, check according to law (General 1143), the amount of endotoxin per 1 unit of glass enzyme should be less than 0.20EU.
Last Update：2022-01-01 13:38:26
Hyaluronidase - Titer determination
Authoritative Data Verified Data
as measured by the glass enzyme assay (General 1207), it was obtained.
Last Update：2022-01-01 13:38:26
Hyaluronidase - Category
Authoritative Data Verified Data
mucopolysaccharide decomposition enzyme.
Last Update：2022-01-01 13:38:26
Hyaluronidase - Storage
Authoritative Data Verified Data
sealed, stored in a cool and dry place.
Last Update：2022-01-01 13:38:27
Hyaluronidase - Legal requirements
Authoritative Data Verified Data
This product should be extracted from the Quarantine qualified mammalian testis, the species of the animal used should be clear, the production process should comply with the current version of the "good manufacturing practice" requirements.
Last Update：2022-01-01 13:38:27
Hyaluronidase - Hyaluronidase for Injection
Authoritative Data Verified Data
This product is a lyophilized sterile product with the addition of a suitable excipient for the use of a glass enzyme. The titer of the hyaluronidase should be between 90.0% and 120.0% of the label amount.
trait
This product is a white or off-white lyophilized cake or powder.
identification
The same results were obtained according to the identification test under the item of glass enzyme.
examination
- the pH value of this product shall be measured according to law (General rule 0631) after each 2ml of water is added for dissolution. The pH value shall be 6.0~7.5.
- loss on drying: take about O.lg of this product and dry it in a phosphorus pentoxide dryer under reduced pressure for 4 hours. The loss of weight shall not exceed 5.0% (General rule 0831).
- bacterial endotoxin should be checked according to the method under the item of glass enzyme, and it should be in accordance with the regulations.
- others should comply with the relevant provisions under injection (General 0102).
titer determination
take 5 pieces of this product, add appropriate amount of cold hydrolyzed gelatin diluent (General rule 1207) to dissolve, and transfer the whole amount to the same 100ml measuring flask, dilute to the scale with the above diluent, shake well. An appropriate amount is taken in a precise amount, and a solution containing about 1.5 units per 1 ml is prepared by quantitative dilution with the above diluent, and the measurement is carried out according to the glass enzyme assay method (General 1207).
category
Same as glass enzyme.
specification
(1)150 units (2)1500 units
storage
sealed, stored in a cool and dry place.
Last Update：2022-01-01 13:38:28
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Product Name: Hyaluronidase Visit Supplier Webpage Request for quotationCAS: 37326-33-3
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Featured ProductsMultiple SpecificationsSpot supply
Product Name: Hyaluronidase Visit Supplier Webpage Request for quotationCAS: 37326-33-3
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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Raw Materials for Hyaluronidase