| biological activity | adh295 is a potent, irreversible and selective inhibitor of NIMA-related kinase 2 (Nek2), the IC50 was 770 nM. FH 295 inhibits cell Nek2 by alkylation of cys22. FH 295 is not active on the mitotic kinases Cdk1,Aurora B or Plk1 and does not interfere with the bipolar spindle assembly or spindle assembly checkpoint. |
| Target | IC50: 770 nM (Nek2) |
|
Cell Line:
| RPMI7951 cells |
|
Concentration:
| 0.08 μM, 0.25 μM, 0.74 μM, 2.2 μM, 6.6 μM, 20 μM |
|
Incubation Time:
| 45 minutes |
|
Result:
| Inhibited WT Nek2 in cells with an IC50 of ~1.3 μM, whereas had little effect on the C22V mutant. |
