MECOBALAMIN
Mecobalamin
CAS: 13422-55-4
Molecular Formula: C63H90CoN13O14P
MECOBALAMIN - Names and Identifiers
| Name | Mecobalamin |
| Synonyms | Algobaz Mecobalamin MECOBALAMIN Vitamin B12 MECOBALAMINE Methylcobalamin METHYLCOBALAMIN Methyl Vitamin B12 cobalt-methylcobalamin Methyl-5,6-dimethylbenzimidazolylcobalamin cobinamide,cobalt-methylderivative,hydroxide,dihydrogenphosphate(ester), |
| CAS | 13422-55-4 |
| EINECS | 236-535-3 |
| InChI | InChI=1/C62H90N13O14P.CH3.Co/c1-29-20-39-40(21-30(29)2)75(28-70-39)57-52(84)53(41(27-76)87-57)89-90(85,86)88-31(3)26-69-49(83)18-19-59(8)37(22-46(66)80)56-62(11)61(10,25-48(68)82)36(14-17-45(65)79)51(74-62)33(5)55-60(9,24-47(67)81)34(12-15-43(63)77)38(71-55)23-42-58(6,7)35(13-16-44(64)78)50(72-42)32(4)54(59)73-56;;/h20-21,23,28,31,34-37,41,52-53,56-57,76,84H,12-19,22,24-27H2,1-11H3,(H15,63,64,65,66,67,68,69,71,72,73,74,77,78,79,80,81,82,83,85,86);1H3;/q;-1;+3/p-2/t31?,34-,35-,36-,37+,41+,52?,53?,56?,57+,59-,60+,61+,62+;;/m1../s1 |
| InChIKey | JEWJRMKHSMTXPP-WYVZQNDMSA-L |
MECOBALAMIN - Physico-chemical Properties
| Molecular Formula | C63H90CoN13O14P |
| Molar Mass | 1343.4 |
| Melting Point | >190°C (dec.) |
| Water Solubility | Partially soluble in cold water, hot water. |
| Solubility | DMSO (Slightly), Methanol (Sparingly), Water (Slightly) |
| Appearance | Red powder |
| Color | Dark Red |
| pKa | pK1:7.64(+1) (25°C) |
| Storage Condition | Sealed in dry,Store in freezer, under -20°C |
| Stability | Light Sensitie |
| Sensitive | Sensitive to light |
| MDL | MFCD00082483 |
| Physical and Chemical Properties | This product is a deep red crystal or crystalline powder, soluble in water, ethanol-soluble, insoluble in acetone, see light unstable |
| Use | For the treatment of nervous system diseases, relieve pain and numbness, rapid relief of neuralgia, improve the pain caused by cervical spondylosis, treatment of sudden deafness |
| In vitro study | Methylcobalamin (CH3-B12) is a cobalamin, a form of vitamin B12. It differs from cyanocobalamin in that the cyanide is replaced by a methyl group. Methylcobalamin (CH3-B12) features an octahedral cobalt(III) centre. Methylcobalamin (CH3-B12) can be obtained as bright red crystals. From the perspective of coordination chemistry, Methylcobalamin (CH3-B12) is notable as a rare example of a compound that contains metal-alkyl bonds. Methylcobalamin (CH3-B12) is equivalent physiologically to vitamin B12, and can be used to prevent or treat pathology arising from a lack of vitamin B12 (vitamin B12 deficiency), such as pernicious anemia. Methylcobalamin (CH3-B12) is also used in the treatment of peripheral neuropathy, diabetic neuropathy, and as a preliminary treatment for amyotrophic lateral sclerosis. From Wikipedia. |
MECOBALAMIN - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S24/25 - Avoid contact with skin and eyes. S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| WGK Germany | 2 |
| RTECS | GG3745000 |
| HS Code | 29362600 |
| Toxicity | LD50 oral in rat: > 5gm/kg |
MECOBALAMIN - Standard
Authoritative Data Verified Data
This product is Coa-[a-( 5, 6-dimethylbenzolimyl)] -methyloleamide. The content of C63H91CoN13014P shall be between 98.0% and 102.0% calculated as anhydrous.
Last Update：2024-01-02 23:10:35
MECOBALAMIN - Trait
Authoritative Data Verified Data
- This product is a deep red crystal or crystalline powder; There is hygroscopicity; See light is easy to decompose.
- This product is slightly soluble in water or ethanol, and almost insoluble in acetonitrile, acetone or ether.
Last Update：2022-01-01 11:37:54
MECOBALAMIN - Introduction
Easily soluble in water, slightly soluble in ethanol, insoluble in acetone. Mecobalamin decomposes when it sees light.
Last Update：2022-10-16 17:13:29
MECOBALAMIN - Differential diagnosis
Authoritative Data Verified Data
- take about 1 mg of this product, add about 50mg of potassium hydrogen sulfate, put it in the crucible, burn to melt, let it cool, add 3ml of water, boil to dissolve, add 1 drop of phenolphthalein indicator solution, sodium hydroxide solution was added dropwise until pale red color was observed. 0.5 of sodium acetate, 0.2% of dilute acetic acid and 0.5 of sodium 1-nitroso-2-naphthol -3, 6-disulfonate solution were added, that is, red or orange-red; Add 0.5 hydrochloric acid, boil for 1 minutes, the color does not disappear.
- in the chromatogram recorded under the content determination item, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the reference solution.
- light-shielding operation. Take the appropriate amount of this product and the reference substance respectively, add water to dissolve and dilute to make a solution containing about 50ug per 1 ml, and determine it by UV-Vis spectrophotometry (General 0401), the absorption spectrum of the test solution in the wavelength range of 220-550nm should be consistent with that of the control solution.
- The infrared absorption spectrum of this product should be consistent with that of the control (Spectrum set 732).
Last Update：2022-01-01 11:37:54
MECOBALAMIN - Exam
Authoritative Data Verified Data
clarity of the solution
take this product 20mg, add water 10ml after dissolution, the solution should be clarified (General Principles 0902 The first method).
Related substances
operation in the dark. Take an appropriate amount of this product, add the mobile phase to dissolve and dilute to make a solution containing about 0.5mg per lml as a test solution; Take lml for precision measurement, put it in a 100ml measuring flask, dilute it to the scale with the mobile phase, as a control solution. According to the chromatographic conditions under the content determination item, 20 u1 of each of the test solution and the control solution are accurately measured and injected into the human liquid chromatograph respectively, and the chromatogram is recorded to 3 times of the retention time of the main component peak. If there are impurity peaks in the chromatogram of the test solution, the area of a single impurity peak shall not be greater than 0.5 times (0.5%) of the area of the main peak of the control solution, the sum of each impurity peak area shall not be greater than 2 times (2.0%) of the main peak area of the control solution.
moisture
take this product, according to the moisture determination method (General 0832 first method 1), the water content shall not exceed 12.0%.
Last Update：2022-01-01 11:37:55
MECOBALAMIN - Content determination
Authoritative Data Verified Data
operation in the dark. Determined by high performance liquid chromatography (General 0512).
chromatographic conditions and system suitability test
silica gel bonded with octa-alkyl silane was used as a filler (4.6mm X 250mm , 5um or equivalent performance column); the mobile phase was 0.03mol / L potassium dihydrogen phosphate solution (adjusted to pH 0.2 with 4.5 mol / L sodium hydroxide solution or phosphoric acid)-acetonitrile (84:16); The detection wavelength was 342nm. Take about 10mg of mecobalamin reference substance, put it in a 20ml measuring flask, add water to dissolve and dilute to the scale, shake well, place it under natural light for 5-10 minutes, and inject 20u1 into the liquid chromatograph, adjust the flow rate so that the retention time of mecobalamin peak is about 12 minutes, record the chromatogram, the separation degree of mecobalamin peak and hydroxycobalamin peak (relative retention time is about 0.2) should be greater than 20, the degree of separation between the mecobalamin peak and the adjacent impurity peak shall meet the requirements.
assay
take an appropriate amount of this product, accurately weigh it, add the mobile phase to dissolve and quantitatively dilute it to make a solution containing about 50ug per lml, which is used as the test solution, the chromatogram was recorded by injection of 20u1 into human Liquid Chromatograph. Another reference substance of mecobalamin was taken and determined by the same method. According to the external standard method to calculate the peak area, that is.
Last Update：2022-01-01 11:37:56
MECOBALAMIN - Category
Authoritative Data Verified Data
treatment of peripheral neuropathy drugs.
Last Update：2022-01-01 11:37:56
MECOBALAMIN - Storage
Authoritative Data Verified Data
light shielding, sealed storage.
Last Update：2022-01-01 11:37:56
MECOBALAMIN - Note
Authoritative Data Verified Data
protection from light is not more than 5lx.
Last Update：2022-01-01 11:37:57
MECOBALAMIN - Mecobalamin Capsules
Authoritative Data Verified Data
This product contains mecobalamin (C63H91CoN13014P) should be the label amount of 90.0% ~ 110.0%.
trait
The content of this product is white to pink granules or powder.
identification
- in the chromatogram recorded under the content determination item, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the reference solution.
- light-shielding operation. Take an appropriate amount of the contents of this product, add water to dissolve and quantitatively dilute to make a solution containing about 50ug of mecobalamin per 1 ml, filter, take the continued filtrate, according to ultraviolet-visible spectrophotometry (General 0401); in addition, an appropriate amount of mecobalamin reference substance was taken, dissolved by adding water and quantitatively diluted to prepare a solution containing about 50ug per 1 ml, and determined by the same method. The absorption spectrum of the test solution in the wavelength range of 220-550nm should be basically consistent with that of the reference solution, with the maximum absorption at the wavelengths of 266nm, 342nm and 522nm.
examination
- the related substances were protected from light. Take an appropriate amount of the contents of this product (about 5mg of mecobalamin), add 10ml of mobile phase with precision, dissolve mecobalamin with ultrasound, filter, and take the continued filtrate as the test solution; Take 1ml with precision, set in a 100ml measuring flask, dilute to the scale with the mobile phase, and shake to serve as a control solution. According to the chromatographic conditions under the content determination item, 20 u1 of each of the test solution and the control solution are accurately measured and injected into the human liquid chromatograph respectively, and the chromatogram is recorded to 3 times of the retention time of the main component peak. If there are impurity peaks in the chromatogram of the test solution, the area of a single impurity peak shall not be greater than the area of the main peak of the control solution (1.0% ) , the sum of each impurity peak area shall not be greater than 3 times (3.0%) of the main peak area of the control solution.
- Content uniformity protected from light. Take 1 capsule of this product, pour the content into a 10ml measuring flask, wash the capsule shell with mobile phase, wash the same measuring flask with washing solution, add the appropriate amount of mobile phase, ultrasonic to dissolve mecobalamin, cool, dilute to scale with mobile phase, shake, filter, take and continue the filtrate, and determine the content according to the method under the content determination item, which shall comply with the regulations (General rule 0941).
- dissolution rate protected from light. Take this product, according to the dissolution and release determination method (General rule 0931 second method), with water as the dissolution medium, the rotation speed is 50 rpm, according to the law operation. After 30 minutes, 10ml of the solution was taken, filtered, and the filtrate was taken as the test solution; An appropriate amount of mecobalamin reference substance was taken, dissolved with water and quantitatively diluted to prepare a solution containing about 1 ml, as a control solution. According to the chromatographic conditions under the content determination item, 100 u1 of each of the above two solutions is accurately measured, respectively injected into human liquid chromatography, the chromatogram is recorded, and the dissolution amount of each particle is calculated by peak area according to external standard method. The limit is 80% of the labeled amount and shall be in accordance with the provisions.
- others should comply with the relevant provisions under the capsule (General 0103).
Content determination
- light-shielding operation. Determined by high performance liquid chromatography (General 0512).
- chromatographic conditions and system suitability test using eighteen alkyl silane bonded silica gel as filler (Luna C18 column, 4.6mm X 250mm ,5um or equivalent column); the mobile phase was 0.03mol / L potassium dihydrogen phosphate solution (adjusted to pH 0.2 with 4.5 mol / L sodium hydroxide solution or phosphoric acid)-acetonitrile (84:16); The detection wavelength was 342nm. Take about 10mg of mecobalamin reference substance, put it in a 20ml measuring flask, add water to dissolve and dilute to the scale, shake well, place it under natural light for 5-10 minutes, and inject 20u1 into human liquid chromatograph, adjust the flow rate so that the retention time of mecobalamin peak is about 12 minutes, record the chromatogram, the separation degree of mecobalamin peak and hydroxycobalamin peak (relative retention time is about 0.2) should be greater than 20, the degree of separation between the mecobalamin peak and the adjacent impurity peak shall meet the requirements.
- determination of 25 capsules of this product, pour out the content, precision weighing, fine, precision weighing an appropriate amount (equivalent to 5mg of mecobalamin), put it in a 100ml measuring flask, add an appropriate amount of mobile phase, ultrasound to dissolve mecobalamin, cool, dilute to scale with mobile phase, shake, filter, take the continued filtrate as a test solution, take 20 u1 precision, inject human liquid chromatography, record the chromatogram; Take an appropriate amount of mecobalamin reference substance, precisely weigh, add mobile phase to dissolve and quantitatively dilute to make a solution containing about 50ug per 1 ml, and determine with the same method. According to the external standard method to calculate the peak area, that is.
category
Same as mecobalamin.
specification
0.5mg
storage
light shielding, sealed storage.
remarks
protection from light is not more than 5lx.
Last Update：2022-01-01 11:37:58
MECOBALAMIN - Reference Information
| Physiological function | Mecobalamin is a therapeutic drug for peripheral neurological disorders. Compared with other vitamin B12 preparations, it has good transmissibility to nerve tissue. It can promote nucleic acid-protein-lipid metabolism and repair damaged nerve tissue through methyl conversion reaction. It plays a coenzyme role in the synthesis of methionine from homocysteine, especially in the synthesis of thymidine from deoxyuridine, and promotes the synthesis of DNA and RNA. In addition, in the experiment of glial cells, this drug increases the activity of methionine synthase and promotes the synthesis of myelin lipid lecithin. Improving the metabolic disorders of nerve tissue can promote the synthesis of axons and their proteins, make the delivery speed of bone proteins close to normal, and maintain the function of axons. mecobalamin injection can also inhibit the abnormal excitation conduction of nerve tissue, promote the maturation and division of red blood mother cells, and improve anemia. Mecobalamin can quickly restore the red blood cell count, hemoglobin and hematocrit values of rats reduced by B12 deficiency. It is applied to megaloblastic anemia and peripheral nerve disorders caused by vitamin B12 deficiency. |
| pharmacological action | mecobalamin is a vitamin B12 derivative, named by chemical structure, it should be called "methyl vitamin B12", its methylated functional group can participate in the body's biochemical process Methyl transfer activity has a promoting effect on the metabolism of nucleic acid, protein and fat in nerve tissue, can stimulate the synthesis of lecithin in Schwann cells, repair damaged myelin, improve nerve conduction velocity; it can directly enter nerve cells and stimulate axon damaged areas to regenerate; stimulate the protein synthesis of nerve cells, strengthen axon anabolism, and prevent axon degeneration; participate in nucleic acid synthesis and promote hematopoietic function. Clinically, it is often used in the treatment of diabetic neuropathy, and long-term use also has a certain effect on the macrovascular complications of diabetes. Mecobalamin is mainly used for peripheral nerve diseases caused by diabetes and megaloblastic anemia caused by vitamin B12 deficiency. It is widely used clinically with few side effects. |
| preparation | the existing synthesis methods of mecobalamin are two steps of cyanocobalamin reduction and methylation. the actual operation is a one-pot reaction. the common synthesis methods mainly include: the preparation method of mecobalamin by sodium borohydride reduction and methyl iodide methylation using cyanocobalamin as raw material; The preparation method of mecobalamin by sodium borohydride reduction and dimethyl sulfate methylation using cyanocobalamin as raw material; using cyanocobalamin as a raw material to reduce by sodium borohydride and methylate methyl toluenesulfonate to prepare mecobalamin; in the presence of cobalt salt or iron salt, sodium borohydride reduces cyanocobalamin, and then use trimethyl iodide The method of methylation of sulfoxide or trimethyl bromide to prepare mecobalamin; the method of reducing cyanocobalamin with sodium borohydride and methylation of dimethyl carbonate to prepare cyanocobalamin. |
| biological activity | Methylcobalamin (Mecobalamin, Vancomin, Methycobal, Cobametin) is one of the active forms of vitamin B12, which can directly participate in homocysteine metabolism. It is used to treat some nutritional diseases and other clinical diseases such as Alzheimer's disease and rheumatoid arthritis. |
| use | used to treat neurological diseases, relieve pain and numbness, quickly relieve neuralgia, improve pain caused by cervical spondylosis, and treat sudden deafness. mecobalamin is an endogenous coenzyme B12, which participates in a one-carbon unit cycle and plays an important role in the transmethylation reaction of methionine from homocysteine. |
Last Update：2024-04-09 20:52:54
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Product Name: Mecobalamin Request for quotation
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Mobile: 0086 189 4982 3763
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CAS: 13422-55-4
Tel: 0086-551-65418684
Email: sales@tnjchem.com
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Mobile: 0086 189 4982 3763
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