N-(3,5-dimethylphenyl)-3,5-dimethylaniline

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Names and Identifiers

Name	3,3',5,5'-Tetramethylbenzidine
Synonyms	TMB TMB-B TMB-E 3355TMB TMB EASY Tmb Quick 4,4'-Bi-2,6-Xylidine Tetramethylbenzidine 3,3,5,5-Tetramethylbenzidine 3,5,3',5'-Tetramethylbenzidine 3,3',5,5'-Tetramethylbenzidine TMB BLUE ONE-STEP SUBSTRATE SYSTEM N-(3,5-dimethylphenyl)-3,5-dimethylaniline 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine 4,4'-Diamino-3,3',5,5'-Tetramethylbiphenyl 1'-biphenyl)-4,4-diamine,3,3',5,5'-tetramethyl-( 1'-Biphenyl)-4,4-Diamine,3,3',5,5'-Tetramethyl-( 1'-Biphenyl]-4,4'-Diamine,3,3',5,5'-Tetramethyl-[ 1'-Biphenyl)-4,4'-Diamine,3,3',5,5'-Tetramethyl-( 1'-biphenyl)-4,4'-diamine,3,3',5,5'-tetramethyl-( 1'-biphenyl]-4,4'-diamine,3,3',5,5'-tetramethyl-[ 1'-Biphenyl]-4,4'-Diamine,3,3',5,5'-Tetramethyl-[1
CAS	54827-17-7
EINECS	259-364-6
InChI	InChI=1/C16H19N/c1-11-5-12(2)8-15(7-11)17-16-9-13(3)6-14(4)10-16/h5-10,17H,1-4H3

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Physico-chemical Properties

Molecular Formula	C16H20N2
Molar Mass	240.34
Density	1
Melting Point	168-171°C(lit.)
Boling Point	100 °C
Flash Point	165.8°C
Water Solubility	Slightly soluble. <0.1 g/100 mL at 20 ºC
Solubility	Dissolved in chloroform and hot methanol, insoluble in water
Vapor Presure	7.56E-05mmHg at 25°C
Appearance	White or yellow solid
Color	Cream
BRN	2808541
pKa	4.49±0.10(Predicted)
Storage Condition	2-8°C
Stability	Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents.
Sensitive	Light Sensitive
Refractive Index	1.5519 (estimate)
MDL	MFCD00007748
Physical and Chemical Properties	Melting Point: 168-169 ℃ water solubility:<0.1g/100 mL at 20 C appearance: white or off-white crystalline powder
Use	This product is for scientific research only and shall not be used for other purposes.

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Risk and Safety

Risk Codes	R36/38 - Irritating to eyes and skin. R36/37/38 - Irritating to eyes, respiratory system and skin. R22 - Harmful if swallowed R41 - Risk of serious damage to eyes
Safety Description	S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S36/37 - Wear suitable protective clothing and gloves. S39 - Wear eye / face protection.
UN IDs	UN 2796 8/PG 2
WGK Germany	3
RTECS	DV2300000
FLUKA BRAND F CODES	8-10
TSCA	Yes
HS Code	29215990
Hazard Class	6.1

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Introduction

Dye for peroxidase. It is a new and safe chromogen reagent. Compared with traditional chromogen reagents such as benzidine, o-tolidine and o-phenylenediamine, TMB has the advantages of high detection sensitivity, good stability and safe use. The results of animal test and Ames bacterial automatic variability test show that TMB is non-carcinogenic and non-mutagenic..

Last Update：2022-10-16 17:26:13

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Reference Information

EPA chemical information	Information provided by: ofmpub.epa.gov (external link)
properties	3,3 ',5,5'-tetramethylbenzidine is a white crystalline powder, odorless, tasteless, hardly soluble in water, easily soluble in organic solvents such as acetone, ether, dimethyl sulfoxide, dimethyl formamide, etc.
preparation	3,3,5,5-tetramethylbenzidine is an important chromogen reagent. It can be 2,6-dimethylaniline as raw material, after activation, oxidative coupling and purification, to obtain pure 3,3,5,5-tetramethylbenzidine, the total yield reaches 65%.
biological activity	TMB (BM blue) is a color substrate for immunohistochemistry and ELISA.
use	this product is a non-carcinogenic substitute for benzidine 1 (negative Ames test), suitable as a peroxidase substrate for enzyme-linked immunosorbent assay. The substrate produces a soluble light blue end product that can be read by spectrophotometry at 370 or 620-650nm. The TMB reaction can be terminated with 2MH2SO4 (turning yellow) and the data is read at 450nm. A sensitive and specific reagent used to detect blood, determine hemoglobin, and determine peroxidase. A new and safe chromogen reagent; TMB has been gradually replacing the strong carcinogen benzidine and other carcinogenic benzidine derivatives, and has been used in clinical laboratory, forensic examination, criminal detection and environmental monitoring. Especially in clinical biochemical tests, TMB, as a new substrate for peroxidase, has been widely used in enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA); mainly used in the following items: occult blood fingerprint detection; rapid detection of alcohol in saliva; preparation of urine test paper; hepatitis virus detection; pregnancy test; rapid determination of glucose, hemoglobin, and albumin in blood and urine; fecal occult blood Test; determination of granulocyte value in blood, detection of steroids, sex hormones; determination of enzyme activity; antigen, antibody and genetic material
toxic substance data	information provided by: pubchem.ncbi.nlm.nih.gov (external link)

Last Update：2024-04-10 22:29:15

N-(3,5-dimethylphenyl)-3,5-dimethylaniline

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N-(3,5-dimethylphenyl)-3,5-dimethylaniline

N-(3,5-dimethylphenyl)-3,5-dimethylaniline

3,3',5,5'-Tetramethylbenzidine

CAS: 54827-17-7

Molecular Formula: C16H20N2

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Names and Identifiers

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Physico-chemical Properties

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Risk and Safety

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Introduction

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Reference Information

Supplier List

N-(3,5-dimethylphenyl)-3,5-dimethylaniline Request for Quotation

3,3',5,5'-Tetramethylbenzidine

CAS: 54827-17-7

Molecular Formula: C16H20N2

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Names and Identifiers

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Physico-chemical Properties

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Risk and Safety

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Introduction

N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Reference Information

Supplier List

N-(3,5-dimethylphenyl)-3,5-dimethylaniline