N-(3,5-dimethylphenyl)-3,5-dimethylaniline
3,3',5,5'-Tetramethylbenzidine
CAS: 54827-17-7
Molecular Formula: C16H20N2
N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Names and Identifiers
| Name | 3,3',5,5'-Tetramethylbenzidine |
| Synonyms | TMB TMB-B TMB-E 3355TMB TMB EASY Tmb Quick 4,4'-Bi-2,6-Xylidine Tetramethylbenzidine 3,3,5,5-Tetramethylbenzidine 3,5,3',5'-Tetramethylbenzidine 3,3',5,5'-Tetramethylbenzidine TMB BLUE ONE-STEP SUBSTRATE SYSTEM N-(3,5-dimethylphenyl)-3,5-dimethylaniline 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine 4,4'-Diamino-3,3',5,5'-Tetramethylbiphenyl 1'-biphenyl)-4,4-diamine,3,3',5,5'-tetramethyl-( 1'-Biphenyl)-4,4-Diamine,3,3',5,5'-Tetramethyl-( 1'-Biphenyl]-4,4'-Diamine,3,3',5,5'-Tetramethyl-[ 1'-Biphenyl)-4,4'-Diamine,3,3',5,5'-Tetramethyl-( 1'-biphenyl)-4,4'-diamine,3,3',5,5'-tetramethyl-( 1'-biphenyl]-4,4'-diamine,3,3',5,5'-tetramethyl-[ 1'-Biphenyl]-4,4'-Diamine,3,3',5,5'-Tetramethyl-[1 |
| CAS | 54827-17-7 |
| EINECS | 259-364-6 |
| InChI | InChI=1/C16H19N/c1-11-5-12(2)8-15(7-11)17-16-9-13(3)6-14(4)10-16/h5-10,17H,1-4H3 |
N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Physico-chemical Properties
| Molecular Formula | C16H20N2 |
| Molar Mass | 240.34 |
| Density | 1 |
| Melting Point | 168-171°C(lit.) |
| Boling Point | 100 °C |
| Flash Point | 165.8°C |
| Water Solubility | Slightly soluble. <0.1 g/100 mL at 20 ºC |
| Solubility | Dissolved in chloroform and hot methanol, insoluble in water |
| Vapor Presure | 7.56E-05mmHg at 25°C |
| Appearance | White or yellow solid |
| Color | Cream |
| BRN | 2808541 |
| pKa | 4.49±0.10(Predicted) |
| Storage Condition | 2-8°C |
| Stability | Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents. |
| Sensitive | Light Sensitive |
| Refractive Index | 1.5519 (estimate) |
| MDL | MFCD00007748 |
| Physical and Chemical Properties | Melting Point: 168-169 ℃ |
| Use | This product is for scientific research only and shall not be used for other purposes. |
N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Risk and Safety
| Risk Codes | R36/38 - Irritating to eyes and skin. R36/37/38 - Irritating to eyes, respiratory system and skin. R22 - Harmful if swallowed R41 - Risk of serious damage to eyes |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S36/37 - Wear suitable protective clothing and gloves. S39 - Wear eye / face protection. |
| UN IDs | UN 2796 8/PG 2 |
| WGK Germany | 3 |
| RTECS | DV2300000 |
| FLUKA BRAND F CODES | 8-10 |
| TSCA | Yes |
| HS Code | 29215990 |
| Hazard Class | 6.1 |
N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Introduction
Dye for peroxidase. It is a new and safe chromogen reagent. Compared with traditional chromogen reagents such as benzidine, o-tolidine and o-phenylenediamine, TMB has the advantages of high detection sensitivity, good stability and safe use. The results of animal test and Ames bacterial automatic variability test show that TMB is non-carcinogenic and non-mutagenic..
Last Update：2022-10-16 17:26:13
N-(3,5-dimethylphenyl)-3,5-dimethylaniline - Reference Information
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| properties | 3,3 ',5,5'-tetramethylbenzidine is a white crystalline powder, odorless, tasteless, hardly soluble in water, easily soluble in organic solvents such as acetone, ether, dimethyl sulfoxide, dimethyl formamide, etc. |
| preparation | 3,3,5,5-tetramethylbenzidine is an important chromogen reagent. It can be 2,6-dimethylaniline as raw material, after activation, oxidative coupling and purification, to obtain pure 3,3,5,5-tetramethylbenzidine, the total yield reaches 65%. |
| biological activity | TMB (BM blue) is a color substrate for immunohistochemistry and ELISA. |
| use | this product is a non-carcinogenic substitute for benzidine 1 (negative Ames test), suitable as a peroxidase substrate for enzyme-linked immunosorbent assay. The substrate produces a soluble light blue end product that can be read by spectrophotometry at 370 or 620-650nm. The TMB reaction can be terminated with 2MH2SO4 (turning yellow) and the data is read at 450nm. A sensitive and specific reagent used to detect blood, determine hemoglobin, and determine peroxidase. A new and safe chromogen reagent; TMB has been gradually replacing the strong carcinogen benzidine and other carcinogenic benzidine derivatives, and has been used in clinical laboratory, forensic examination, criminal detection and environmental monitoring. Especially in clinical biochemical tests, TMB, as a new substrate for peroxidase, has been widely used in enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA); mainly used in the following items: occult blood fingerprint detection; rapid detection of alcohol in saliva; preparation of urine test paper; hepatitis virus detection; pregnancy test; rapid determination of glucose, hemoglobin, and albumin in blood and urine; fecal occult blood Test; determination of granulocyte value in blood, detection of steroids, sex hormones; determination of enzyme activity; antigen, antibody and genetic material |
| toxic substance data | information provided by: pubchem.ncbi.nlm.nih.gov (external link) |
Last Update：2024-04-10 22:29:15
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