Name | PHYTOHEMAGGLUTININ PHA-M |
Synonyms | PHA-C PHA-P BOUND PHA-M PHA-L AGAROSE Phytolectin fitohemaglutynina phytohemagglutinin phytohemaglutinins PHYTOHEMAGGLUTININ PHA-M |
CAS | 9008-97-3 |
EINECS | TJ3470000 |
Water Solubility | PBS: 2 mg/mL |
Storage Condition | 2-8 ℃, dark |
Use | Uses PHA is a mitogen (mitogen). In vitro can stimulate the small lymphocytes into lymphocytes with immune activity, and promote its division. |
Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
Reference Show more | 1. Xin Bing Xu, Chenglin Li, Xingming Chen et al. Protective effect of resveratrol on human peripheral blood lymphocytes irradiated by 60Co gamma ray in vitro [J]. China Occupational Medicine, 2012(02):122-124. 2. Huang Dongyu, Liu Mingsheng, Wang Jian, et al. Effects of selenium poisoning on immune function of goslings [J]. Jiangsu Agricultural Sciences, 2015, 43(002):218-220. 3. Shi Linlin, Xu Xiaohui, Zhang Long, etc. Comparative analysis of chromosome karyotype between male and female Procambarus clarkii [J]. Journal of Anhui Agricultural University, 2019, v.46;No.168(02):32-39. 4. Liu kuanhui, Tian Weijun, Gao Zhenzhen, etc. Comparative study on enhancement of immune function of chicken by polysaccharide-selenium polysaccharide compound [J]. Journal of Nanjing Agricultural University, 2017(3). 5. Wang, C., li, M., wang, P. et al. An electrochemiluminescence biosensor based on boron nitride quantum dots as novel coreactant for quantitative determination of concanavalin A. Microchim Acta 187, 409 (2020). https://doi.org/10.1007/s00604-020-04385-6 6. Wang, C., Li, M., Wang P. et al. An electrochemiluminescence biosensor based on boron nitride quantum dots as novel coreactant for quantitative determination of concanavalin A. Microchim Acta 187, 409 (2020). https://doi.org/10.1007/s00604-020-04385-6 7. Wu, Hong, et al. "Construction and functional analysis of an anti-human cervical carcinoma/anti-human CD3 single-chain bispecific antibody." Molecular medicine reports 14.1 (2016): 804-810.https://doi.org/10.3892/mmr.2016.5292 8. [IF=2.1] Hong Wu et al."Construction and functional analysis of an anti-human cervical carcinoma/anti-human CD3 single-chain bispecific antibody."Mol Med Rep. 2016 Jul;14(1):804-810 9. [IF=7.561] Zhou Xu et al."Expression and Function Analysis of Interleukin-17A/F1, 2, and 3 Genes in Yellow Catfish (Pelteobagrus fulvidraco): Distinct Bioactivity of Recombinant IL-17A/F1, 2 and 3."Front Immunol. 2021 Jun;0:2564 10. [IF=6.953] Yuan-Hong Hu et al."Molecular characterization, expression analysis and function identification of Pf_TNF-α and its two receptors Pf_TNFR1 and Pf_TNFR2 in yellow catfish (Pelteobagrus fulvidraco)."Int J Biol Macromol. 2021 Aug;185:176 11. [IF=5.89] Xu Wenting et al."A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish."Front Bioeng Biotech. 2021 Jan;0:1481 12. [IF=5.833] Wang Caixia et al."An electrochemiluminescence biosensor based on boron nitride quantum dots as novel coreactant for quantitative determination of concanavalin A."Microchim Acta. 2020 Jul;187(7):1-9 |
painted festive , Lin chanzhu , Ye Meili ,< a href = "https://xueshu.baidu.com/usercenter/data/author?cmd=authoruri&wd=authoruri:(1 e3201952ae0195b) author:(Huang Shaoyong)" target = "_blank"> Huang Shaoyong
Summary:
the effect of phytohemagglutinin PHA acid granules on sperm was detected after intercourse, which provided scientific basis for the transition of external contraceptive drugs to clinical trial observation. 1. detection method 1. object selection: select healthy couples with reproductive history who live together aged 23-43 years old, and the woman's obstetrics and gynecology examination
keywords:
scientific basis plant hemagglutinin sperm agglutination oral contraceptives cervical canal detection method clinical trial swimming sperm external use fertility history
cited:
Year:
1988
patent for invention
application (patent) number:
CN98115781.5
date of application:
19980706
public/announcement number:
CN1240670A
date of publication/announcement:
20000112
Applicant (patentee):
Fujian Institute of Material Structure, Chinese Academy of Sciences
Inventor:
song lijun , Lin Yujuan , Cai Jianhua
national and provincial code:
CN350102
Summary:
the simple separation and purification technology of phytohemagglutinin (Phytohemagg lutinin PHA-E) is to obtain high purity PHA-E by using only one S-Sepharose Fast Flow(SSFF) strong cation column after one gradient elution after extraction of salt solution. The in-column filler can be reused after simple treatment. The obtained PHA-E freeze-dried powder grows crystals under Tris-HCl buffer and PEG conditions, making the PHA-E reach 100% purity. PHA-E can treat a variety of tumors and aplastic anemia in combination with other drugs.