PUC19

PUC19 - Names and Identifiers

Name	PUC19
Synonyms	PUC19 THYMUS NUCLEIC ACID deoxyribonucleicac. Nucleicacids,deoxyribo Desoxyribosenucleicacid DEOXYRIBONUCLEIC ACID TYPE XI DEOXYRIBONUCLEIC ACID TYPE XII Amino-terminal FLAG-BAPTM Fusion Protein Deoxyribonucleic Acid not highly polymerized (Herring sperm)
CAS	9007-49-2
EINECS	2017-001-1

PUC19 - Physico-chemical Properties

Molecular Formula	C15H31N3O13P2
Molar Mass	523.366462
Density	1.00g/mLat 20°C
Water Solubility	Dissolve in TE buffer or water at 2mg/ml
Solubility	50 mM Tris pH 8.0: 10mg/mL, clear, faintly brown-yellow
Appearance	Liquid
Color	White to Light yellow to Light orange
Storage Condition	-20°C

PUC19 - Risk and Safety

Safety Description	S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes.
WGK Germany	3
RTECS	HG1933000
FLUKA BRAND F CODES	3-10-21
TSCA	Yes
HS Code	29349990

PUC19 - Introduction

PUC19 (full name is pUC19 plasmid) is a commonly used plasmid, which is widely used in the field of molecular biology research. The following is the nature, use, preparation and safety information of PUC19:

Nature:
1. Size: The size of PUC19 is about 2.7 kilobases (kb).
2. type: it belongs to high copy number plasmid, that is, in most host cells can exist multiple replicates.
3. Vector: PUC19 is a vector plasmid, which can carry exogenous genes. It is usually modified and used for DNA cloning and expression research.

Use:
1. DNA cloning: PUC19 can be used to store and amplify DNA sequences, enabling researchers to perform DNA cloning experiments, such as inserting foreign fragments and constructing DNA libraries.
2. Gene expression: By inserting the target gene sequence into the corresponding site of PUC19, the transcription and translation system of the host cell can be used to express the gene, so as to study its function and properties.
3. DNA sequencing: PUC19 is often used as a template for DNA sequencing reactions to help determine the target DNA sequence.

Preparation Method:
1. Obtaining: pUC19 can be prepared by purchasing commercially available plasmid sources or by genetic engineering techniques.
2. Construction: PUC19 can be constructed by standard molecular biology methods. The common preparation steps include: extracting PUC19 from common hosts such as E. coli, shearing, connecting target DNA fragments, and finally transforming into host cells for amplification.

Safety Information:
PUC19 has no obvious safety hazard under normal experimental conditions. However, any experiments involving DNA should follow relevant laboratory safety procedures to ensure the safety of operators and the environment. Be aware of the relevant biosafety laws and regulations in the area where the laboratory is located, and follow the relevant laboratory practices, especially the regulations on the use and handling of gene technology.

Last Update：2024-04-09 20:45:29