Name | Folin-Phenol |
Synonyms | Phennol Folin-Phenol Foline-Phenol Folin-Ciocalteu'S phenol reagent |
Appearance | Liquid |
Storage Condition | Room Temprature |
Folin-Phenol is a chemical reagent used for determining protein content. It is commonly used for the analysis and quantification of protein concentration, by reacting with aromatic amino acids in proteins (such as tyrosine and phenylalanine) and generating a colorimetric product, the absorbance of which can be measured to determine protein content. This method is also frequently used for determining total protein content in biological samples, such as serum, cell extracts, etc. The Folin-Phenol method is a classic protein quantification method that is widely used in biochemical experiments and research.
Properties: Folin-Phenol is a dark blue liquid with high oxidizing properties. When it reacts with aromatic amino acids in proteins, it produces a deep blue-purple color and is sensitive to light.
Uses: Folin-Phenol is primarily used for protein determination and can be employed in various biochemical experiments. This reagent enables fast and accurate measurement of protein content by reacting with the aromatic amino acids in proteins. It can also be used to detect total protein content in biological samples such as serum and cell extracts.
Preparation: Folin-Phenol is prepared by mixing Folin-Ciocalteu reagent with a phenol solution, typically made from materials like ammonium vanadate phosphate and sodium borate phosphate.
Safety: Folin-Phenol is a strong oxidizing agent with irritant and corrosive properties. It should be handled with care to avoid skin, eye, and respiratory tract contact. Operations should be conducted in a well-ventilated area, and personal protective equipment such as gloves and goggles should be worn. Additionally, Folin-Phenol should be stored away from heat sources and in a cool, dry place.