Pyronine
Pyronin Y
CAS: 92-32-0
Molecular Formula: C17H19ClN2O
Pyronine - Names and Identifiers
| Name | Pyronin Y |
| Synonyms | Pyronine Pyronin G Pyronin Y Pyronine Y C.I. 45005 PYRONINE G Pyronin y(G) Pyronin Y, CI 45005 Pyronin G (Pyronin Y) Pyronin G, PyroninJ Pyronin Y (CI x 45005) pyronin Y molecular biology Pyronin G, PyroninJ, PyroninY Pyronin G, C.I. 45005, for microscopy Pyronin Y,Pyronin G, PyroninJ, PyroninY Pyronin Y,pure,high purity biological stain N-[6-(dimethylamino)-3H-xanthen-3-ylidene]-N-methylmethanaminium chloride |
| CAS | 92-32-0 |
| EINECS | 202-147-8 |
| InChI | InChI=1/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1 |
| InChIKey | INCIMLINXXICKS-UHFFFAOYSA-M |
Pyronine - Physico-chemical Properties
| Molecular Formula | C17H19ClN2O |
| Molar Mass | 302.8 |
| Melting Point | 250-260°C(lit.) |
| Water Solubility | Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL). |
| Solubility | Soluble in water (30 mg/mL), methyl cellosolve (9 mg/mL), and ethanol (3 mg/mL). |
| Appearance | Powder/Solid |
| Color | Pink |
| Maximum wavelength(λmax) | ['548 nm'] |
| Merck | 13,8097 |
| BRN | 3795789 |
| Storage Condition | room temp |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| MDL | MFCD00011725 |
| Use | is a dye that stains RNA red and has been shown to accumulate in the mitochondria of living cells; used for bacterial staining. |
| In vitro study | Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin. A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting. In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells. Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent. Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA). |
Pyronine - Risk and Safety
| Risk Codes | R68 - Possible risk of irreversible effects R36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| WGK Germany | 3 |
| RTECS | BQ1450000 |
| TSCA | Yes |
| HS Code | 32041300 |
Pyronine - Reference
| Reference Show more | 1. Yang Chenghuan, Yang Yao, Li Kuo, et al. Effects of red kidney bean phytohemagglutinin on placental tissue of middle and late pregnancy mice [J]. Chinese Journal of Family Planning, 2015, 23(8). |
Pyronine - Reference Information
| Color index | 45005 |
| Application in biological field | Anti-viral agent; Anti-viral agent; nucleic acid sequencing; herb |
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| biological activity | Pyronin Y (Pyronine G) is a cationic dye that can be inserted into RNA and can be targeted to include RNA, the cellular structure of DNA and organelles. Pyronin Y forms a fluorescent complex with a double-stranded nucleic acid (especially RNA), allowing semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of nucleoprotein complexes in living cells. |
| Use | a cationic dye that intercalates RNA, exhibiting a red band during electrophoresis. |
Last Update：2024-04-09 20:02:46
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