Spectrophotometer - Names and Identifiers
Spectrophotometer - Introduction
A Spectrophotometer is an instrument used to measure the absorption or emission of light by a substance. It can determine the concentration or other related properties of a substance by measuring the intensity of light absorbed or emitted by the substance.
The main properties of the Spectrophotometer include:
1. Wide wavelength range: It can measure a wide range of wavelengths, from ultraviolet to infrared.
2. High accuracy: It can provide high-precision light intensity measurement results.
3. Adjustability: You can select different wavelengths for measurement by adjusting the grating of the light inlet.
4. High sensitivity: can measure very low concentration of samples.
Spectrophotometer is widely used, mainly in the following aspects:
1. Analytical Chemistry: used to determine the concentration of the solution, such as the determination of drug concentration, metal ion concentration.
2. Biochemistry: used to determine the content of protein, nucleic acid, etc. in biological samples.
3. Environmental analysis: used to monitor the concentration of pollutants in the environment, such as water quality monitoring, atmospheric analysis, etc.
4. Medical diagnosis: used to determine the concentration of specific substances in vivo, such as glucose and cholesterol in the blood.
Spectrophotometer preparation method generally includes the following steps:
1. Light source: select the appropriate light source, such as incandescent lamp, deuterium lamp or xenon lamp.
2. Collimation: collimate the light from the light source through the collimation hole to make it a parallel beam.
3. Interference: Interference of light through a grating or prism, decomposing it into components of different wavelengths.
4. Photoelectric conversion: the use of photosensitive elements (such as photodiodes or photomultiplier tubes) to convert optical signals into electrical signals.
5. Signal processing: Use electronic instruments to amplify and process electrical signals to obtain accurate measurement results.
When using Spectrophotometer, there are some security considerations to note:
1. Operators need to wear appropriate protective glasses and gloves to prevent exposure to hazardous chemicals.
2. In the process of Operation should pay attention to prevent the light source heat radiation or light damage to the eyes.
3. Regularly check whether the circuit and optical components of the instrument are normal to ensure safe use.
Last Update：2024-04-10 22:41:30