Molecular Formula | C42H67NO10 |
Molar Mass | 745.98 |
Density | 1.15±0.1 g/cm3(Predicted) |
Melting Point | 169° |
Boling Point | 807.4±65.0 °C(Predicted) |
pKa | 7.8(at 25℃) |
Storage Condition | 2-8℃ |
Physical and Chemical Properties | Chemical properties light grayish white solid crystals with a slightly stale soil smell. The vapor pressure is about 1.3 × 10-10Pa. In the pH 7.74 aqueous solution, the pair of metals and metal ions are relatively stable within 28d. The half-life of photodegradation in soil is 9 ~ 10d. The half-life of hydrophotolysis is less than 1d. In the range of pH 5~7, it is relatively stable in aqueous solution. Half-decay period> 200d at pH 9. Spinosad is a mixture of more than ten components, the main components are A and D. |
Use | Application of new biological pesticides, with a unique chemical structure with both safe and quick-acting characteristics, has been low toxicity, low residue, safety and other characteristics, the United States |
strain new microorganism polybactericidal polyspora (Saccharo-polyspora spinosa) or mutant strain capable of producing spinosad. Aerogenous mycelium was produced on composite medium and synthetic medium. The color of the aerial spore group is mainly light yellow pink, but also white, and the reverse side is yellow to yellowish brown, without obvious pigmentation.
The seed culture medium for fermentation culture of polysporospora spinosae is: enzymatic hydrolysis of soybean hydrolysate 3%, yeast extract 0.3%,MgSO4 · H2O 0.2%, glucose 0.5%, maltose. 4%, deionized water 1L, sterilization at 121 ℃ for 30min. The inoculation amount was 10%. The preferred carbon sources for mass production are glucose and maltose, and ribose, xylose, fructose, galactose, mannose, mannitol, soluble starch, potato dextrin, methyl oleate, oils, etc. can also be used. The preferred nitrogen poise is raffinose, peptone milk and digested soybean powder, fish meal, corn extract, yeast extract, hydrolyzed casein, beef paste, etc. Inorganic salts mixed into the culture medium can produce the following ions of conventional soluble salts: zinc ions, sodium ions, magnesium ions, ammonium ions, chloride ions, carbonate ions, sulfate ions, nitrate ions and so on. In addition, the culture medium should also have the necessary trace elements for the growth and development of microorganisms. If there is a foaming problem, soybean oil can be added to the culture medium to inhibit foaming. The culture temperature of spinosad polypora is 24~33 ℃, and the optimum temperature for producing spinosad is 28~30 ℃. During industrial fermentation, the dissolved oxygen in the tank should be maintained above 60%, preferably above 65%, through ventilation and stirring speed. The pressure in the tank is 0.034MPa.
After separation and extraction, after 7-10 days of fermentation, adding an equal volume of acetone, filtering to remove the bacteria, adjusting the pH value of the filtrate to 13, resin adsorption, gradient elution of spinosad A and D with 0-95% methanol: acetonitrile = 1:1 (containing sodium 0.1% acetate) solution, collecting eluent in sections to obtain spinosad-containing eluent, and concentrating to obtain spinosad concentrated solution. Dilute the concentrated solution with petroleum ether, add silica gel chromatographic column, elute with petroleum ether and methanol gradient, collect in sections, and obtain spinosad A and D eluents.
Analysis and detection of high performance liquid chromatography (HPLC). Column: ODS-AO, liquid dynamic phase: acetonitrile/methanol/water = 40/40/20 (containing 0.05% ammonium acetate), flow rate 3 mL/min, detection wavelength: 250nm.