Name | TANNASE |
Synonyms | TANNASE DANNINGMEI Tannin acyl Hydrolase tannin acyl hydrolase tannase from aspergillus ficuum |
CAS | 9025-71-2 |
EINECS | 232-804-4 |
Storage Condition | 2-8℃ |
MDL | MFCD00212735 |
Physical and Chemical Properties | Chemical properties light black powder. The optimum pH value is 5.5~6.0 and the optimum temperature is 33 ℃. The main function is to add water to decompose tannin into tannic acid, glucose and gallic acid. |
Use | Use enzyme preparation. It is mainly used to decompose tannins in the production of instant tea, in order to improve the cold solubility of the finished product and to avoid turbidity during cooling after hot dissolution. When used in the pH value of 5.5~6 in the tea extract, according to the ratio of adding 2.5g tannase per liter, and then stirred at 30 ℃ for 70min, then the temperature was raised to 90 ° C. To inactivate the enzyme, and the tannase was removed by centrifugation. |
Hazard Symbols | Xn - Harmful |
Risk Codes | 42 - May cause sensitization by inhalation |
Safety Description | S22 - Do not breathe dust. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) |
Raw Materials | Casein Tannic acid |
Reference Show more | 1. Zhang Jinhong, Li June, Wei Xinyuan, etc. Study on different extraction methods of combined phenol from apple pomace [J]. Science and Technology of Food Industry, 2016, 37(020):160-165. 2. Li Zhen, Li Ping. Ultrasonic-assisted enzymatic extraction of Gallic acid from Galla Chinensis and its antioxidant activity [J]. Chinese Journal of Animal Nutrition, 2019(8). 3. [IF = 6.312] Xiao-Yu Xu et al."Effects of Tannase and Ultrasound Treatment on the Bioactive Compounds and antioxant Activity of Green Tea Extract." Antioxidants-Basel. 2019 Sep;8(9):362 4. [IF = 6.312] Xiao-Yu Xu et al."Effects of Food Processing on In Vivo Antioxidant and Hepatoprotective Properties of Green Tea Extracts."Antioxidants-Basel. 2019 Dec;8(12):572 5. [IF=5.645] Linxin Guo et al."Purification and characterization of hydrolysable tannins extracted from Coriaria nepalensis bark using macroporous resin and their application in gallic acid production."Ind Crop Prod. 2021 Apr;162:113302 |
storage conditions | 2-8°C |
morphology | powder |
color | white |
use
enzyme preparation. It is mainly used to decompose the tannin in the production of instant tea to improve the cold solubility of the finished product and avoid turbidity during cooling after hot dissolution. When in use, in the tea extract with a pH value of 5.5~6, add 2.5g of tannase preparation per liter, then stir at 30 ℃ for 70min, then raise the temperature to 90 ℃ to kill the enzyme, centrifuge to remove the tannase.
production method
Generally, Aspergillus niger (Aspergillus niger) or Penicillium grayicum (Penicillium glaucum) are controlled and cultured in Chua's medium (CzapekDox) containing 2% tannic acid and 0.2% casein hydrolysate, and the hyphae are taken out. It is prepared by precipitation with acetone and drying.
WGK Germany | 1 |