| Name | trysin-chymotrypsin |
| Synonyms | Trypsin-ChyMotrypsi trysin-chymotrypsin Trypsin-Chymotrypsin |
| EINECS | 1806241-263-5 |
| Physical and Chemical Properties | Chemical properties Chymotrypsin is a mixed crystal of chymotrypsin and trypsin obtained from germanium pancreas. It has the properties of two enzymes. The crystals are soluble in normal saline, and the aqueous solution is easily inactivated. The pH7 ~ 8 activity is the strongest, and it is relatively stable when dried. The activity of hydrolyzed casein is equivalent to that of bovine α-chymotrypsin, but the activity of hydrolyzed benzoyl tyrosine ethyl ester contained in chymotrypsin is equivalent to that of bovine and sheep chymotrypsin 3 times. The activity of pig trypsin hydrolyzed ester bond is also similar to that of bovine and goat chyme protein. Chymus trypsin for injection is a powder injection prepared by dialysis, precipitation and filtration of chymus trypsin recrystallization filter cake, acetone dehydration for 3 times and then vacuum drying. The chyme trypsin tablet is an enteric-coated film containing chyme trypsin. |
| Use | Uses chymotrypsin can be used to treat a variety of inflammation such as: for chronic bronchitis, bronchial asthma, gastritis, cervicitis, pelvic inflammatory disease, suppurative otitis media, keratitis, prostatitis, Thromboangiitis, cerebral thrombosis, etc. On inflammatory edema, hematoma, postoperative adhesions, ulcers, thrombosis also have a certain effect. However, when injected, local irritation can cause allergic reactions. Do not chew when taking orally, so as not to be destroyed by pepsin and failure. Have severe liver dysfunction and abnormal blood coagulation function disabled. Intramuscular injection, 1~2 times a day, each time 5mg, with 2ml benzyl alcohol injection or normal saline solution after injection, treatment for 10~10 days. For the prevention or control of recurrence, injection 2~3 times a week, each 5mg, external use: with physiological saline solution after gauze wet topical, or mixed with antibiotic ointment coated gauze, daily dressing change 1~2 times. |
take fresh or frozen germanium pancreas, thaw, wash and put it into a meat grinder to grind it, add 2 times the volume of sulfuric acid solution (0.125mol/L) according to the amount of pig pancreas pulp, stir evenly, extract it under stirring for 1h, adjust the right left of PH3, and leave it overnight at 40. The next day, filter with double-layer gauze, filter residue with equal volume of cold sulfuric acid solution (0.125mol/L), repeat extraction once, merge twice to obtain extract.
Porcine pancreas H{2SO4;pH3;40 & rarr; Extract
Segmental salting out under continuous stirring, gradually add a certain amount of industrial ammonium sulfate to the extract to make the saturation of ammonium sulfate reach 25%. Add a small amount of active kaolin to help filter, place it at a low temperature, filter it after 1 day, add ammonium sulfate (CP) to the filtrate, do the same operation as above, obtain the recovery twice, collect the 25% ~ 65% saturation precipitation part, and obtain the crude filter cake.
Extract (NH4)2SO4 & rarr; Segmented salting out crude filter cake
Dialysis and salting out Dissolve the coarse filter cake with 3 times the volume of cold distilled water, dialyze the acetic acid buffer (0.01mol/L) with pH5.5 at low temperature, filter, add a certain amount of ammonium sulfate (CP) to the clear liquid until the saturation of ammonium sulfate reaches 65%, and filter the precipitate to obtain the dialysis filter cake.
Crude filter cake pH5.5; Acetic acid buffer;(NH4)2SO4 & rarr; Dialysis; Salting out dialysis filter cake
Salting out and removing impurities Dissolve the dialysis cake in 3 times the volume of cold distilled water, adjust pH3 ~ 3.5, add a certain amount of ammonium sulfate (CP) until the saturation of ammonium sulfate reaches 25%, and after being placed at room temperature for 3~5h, use 0.5% activated carbon to help filter to obtain clear filtrate. Then, under constant stirring, 100ml of clarified filtrate is slowly added to 10ml of ethanol preheated to about 250 (ethanol concentration reaches 10%), and then filtered after standing at room temperature for 3 hours to obtain clarified filtrate.