Name | Xylo-oligosaccharides |
Synonyms | Xylan oligosaccharide Xylo-oligosaccharides Xylose oligosaccharide |
EINECS | 1592732-453-0 |
Appearance | White to light brown powder or solid |
Storage Condition | Room Temprature |
Reference Show more | 1. Wang Hui, Qin Cuili, Gong Qiang, etc. Effects of non-digestible oligosaccharides on immune regulation in mice [J]. Food and machinery, 2019(9). 2. Zhao Siqi, Qin Cuili, Wang Hui, et al. Effects of three non-digestible oligosaccharides and their combinations on intestinal flora of mice by high-throughput sequencing [J]. Chinese Journal of Nutrition, 2019, v.41(06):62-65 72. 3. Abasubong, Kennedy prucence, et al. "Fishmeal replacement by rice protein concentrate. Li, Hongbo, et al. "Development of a microencapsulated synbiotic product and its application in yoghurt." LWT 122 (2020): 109033.https://doi.org/10.1016/j.lwt.2020.109033 5. [IF=4.581] Kenneth Prudence Abasubong et al."Fishmeal replacement by rice protein concentrate with xylooligosaccharides supplement benefits the growth performance, antioxidant capability and immune responses against Aeromonas hydrophila in blunt snout bream (Megalob 6. [IF=4.242] Kenneth Prudence Abasubong et al."Xylooligosaccharides benefits the growth, digestive functions and TOR signaling in Megalobrama amblycephala fed diets with fish meal replaced by rice protein concentrate."Aquaculture. 2019 Feb;500:417 7. [IF=3.497] K. P. Abasubong et al."Dietary supplementation of xylooligosaccharides benefits the growth performance and lipid metabolism of common carp (Cyprinus carpio) fed high-fat diets."Aquacult Nutr. 2018 Oct;24(5):1416-1424 8. [IF=2.461] Liao Ning et al."Oligosaccharides as co-encapsulating agents: effect on oral Lactobacillus fermentum survival in a simulated gastrointestinal tract."Biotechnol Lett. 2019 Feb;41(2):263-272 9. [IF=6.953] Ziyu Deng et al."Oligosaccharides act as the high efficiency stabilizer for β-galactosidase under heat treatment."Int J Biol Macromol. 2019 Sep;137:69 10. [IF=5.044] Niu Yaping et al."Coexpression of β-xylosidase and xylose isomerase in Saccharomyces cerevisiae improves the efficiency of saccharification and fermentation from xylo-oligosaccharides."Cellulose. 2019 Sep;26(13):7923-7937 11. [IF=4.952] Hongbo Li et al."Development of a microencapsulated synbiotic product and its application in yoghurt."Lwt Food Sci Technol. 2020 Mar;122:109033 12. [IF=4.952] Chenxi Yang et al."Effects of fermentable carbohydrates on the quality properties and in vitro digestibility of Yiyang rice cake."Lwt Food Sci Technol. 2021 Aug;148:111800 13. [IF=4.411] Zhaojun Peng et al."Purification, Identification, and Characterization of an Endo-1,4-β-Xylanase from Wheat Malt."Molecules. 2020 Jan;25(7):1572 14. [IF=3.638] Zhaojun Peng et al."Effect of an endo-1,4-β-xylanase from wheat malt on water-unextractable arabinoxylan derived from wheat."Journal Of The Science Of Food And Agriculture. 2021 Oct 05 15. [IF=3.247] Lumpan Poolsawat et al."Dietary xylooligosaccharide improved growth, nutrient utilization, gut microbiota and disease resistance of tilapia (Oreochromis niloticus x O. aureus)."Anim Feed Sci Tech. 2021 May;275:114872 16. [IF=5.279] Shu-Rui Zhong et al.Novel Antioxidative Wall Materials for Lactobacillus casei Microencapsulation via the Maillard Reaction between the Soy Protein Isolate and Prebiotic Oligosaccharides.J Agr Food Chem. 2021;69(46):13744-13753 17. [IF=1.28] MINGKUI LV et al."In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota."Pol J Microbiol. 2021 Dec; 70(4): 511-520 18. [IF=2.13] Kenneth Prudence Abasubong et al."Effects of dietary xylooligosaccharide prebiotic supplementation on growth, antioxidant and intestinal immune-related genes expression in common carp Cyprinus carpio fed a high-fat diet."Journal Of Animal Physiology And A 19. [IF=9.147] Sheliang Zhao et al."Improving pea protein functionality by combining high-pressure homogenization with an ultrasound-assisted Maillard reaction."Food Hydrocolloid. 2022 May;126:107441 20. [IF=3.497] Cao Kailin et al."Supplementation of a Multienzyme Complex, an Organic Acid-Essential Oil Complex, and Prebiotic Alone or in Combination Affects Growth, Nutrient Utilization, and Immune Function of Rainbow Trout (Oncorhynchus mykiss)."Aquacult Nutr. 2022;2 |
Overview | xylooligosaccharides are widely used in health food industry as prebiotics, it can also be used in foods as a humectant or as a low-calorie additive. The by-products of agricultural products contain abundant xylan resources. Xylooligosaccharides are xylo-oligosaccharide mixtures mainly composed of xylobiose, xylotriose and xylotetraose, which are obtained by hydrolyzing β-1,4 xylosidic bonds with xylan as substrate by xylanase. Corn cob, bagasse and rice husk all have high content of xylan, which is an ideal natural raw material for the preparation of xylooligosaccharides. However, because the natural xylan molecule is a kind of hybrid polysaccharide, the cross-linking between the main chain and the side chain molecules causes the xylan to form a complex network substrate structure which is difficult to be hydrolyzed, the existing enzymatic hydrolysis process of xylan has the problems of low enzymatic hydrolysis efficiency and high process cost. Therefore, there is an urgent need to design a method that can overcome the above defects to some extent. |
hydrolysis | xylooligosaccharides are prepared from lignocellulosic materials containing xylan by chemical methods, direct enzymatic hydrolysis of sensitive substrates, or a combination of chemical and enzymatic treatment. The status of production and application of xylooligosaccharides until 2000 has been reviewed previously. The purpose of this project is to provide an overview of recent developments in the same field and propose a feasible scheme, the protocol comprises at least one water treatment (hot water, compressed water or steam) to cause degradation of xylan to dissolve carbohydrates. when the hydration process (autohydrolysis or hot water treatment) of the Xylan-containing lignocellulosic material is carried out under suitable operating conditions, the hemicellulose chain will decompose under the hydrolysis of hydronium ions to obtain soluble products, and then cellulose and lignin with some chemical changes remain in the solid phase. The autohydrolyzed residual solids can be further utilized, for example, for the enzymatic hydrolysis of cellulose, for feed, fuel or for construction materials. |
Application | a) as a functional food additive B) application in the pharmaceutical industry in medicine, xylooligosaccharides can be used for the prevention and treatment of acute and chronic gastroenteritis, osteoporosis, skin and sputum itching and otitis, in addition, can increase intestinal peristalsis, maintain the water holding capacity of feces, its main principle is the activation of intestinal beneficial bacteria. c) as a feed additive, feeding raw animals and fish with feed containing xylooligosaccharides can improve the immunity of raw animals, reduce various diseases, and shorten the growth cycle of raw animals. d) application in agriculture xylooligosaccharides can be used as a growth stimulator and a growth promoter for crops, as a ripening agent for spraying fruits and vegetables, and can significantly improve the yield of crops. |
preparation | preparation of xylooligosaccharides: 1) preparation of high temperature resistant endoxylan enzyme: the recombinant plasmid pET-28b-CoXynA constructed with the high-temperature-resistant endoxylan enzyme gene was heat-shock transformed into the eschlichiacolitransetta (DE3) host to construct a genetically engineered bacterium expressing the high-temperature-resistant endoxylan enzyme. The above genetic engineering bacteria were cultured at 37 ℃, when the OD600 value of the bacteria reached 0.8, isopropylthiogalactopyranoside (IPTG) was used to induce 6h at 37 ℃, after the bacteria were broken by high pressure homogenization, the supernatant was passed through affinity chromatography column (nickel column), anion exchange column and molecular sieve (Superdex75). After the preparation of high temperature resistant xylanase enzyme solution (5.1 mg/mL). The number of the thermostable endoxylan enzyme in the NCBI database is adq03732. 2) preparation of bifunctional xylan hydrolase: The recombinant plasmid pET-28b-CLH10 constructed with bifunctional xylan hydrolase gene was transformed into EscherichiacoliBL21-Gold(DE3) host by heat shock, construction of genetic engineering bacteria expressing bifunctional xylan hydrolase. The above genetic engineering bacteria were cultured at 37 ℃, and when the OD600 value of the bacteria reached 0.8, Cooling to 18 ℃, under the condition of 18 ℃, using isopropylthiogalactopyranoside (IPTG) induced 12H, after the high pressure homogenization method to break the bacteria, after the supernatant of the bacterial solution was sequentially subjected to affinity chromatography column (nickel column) and molecular sieve (Superdex75), a bifunctional xylan hydrolase solution (4.9mg/mL) was prepared. The number of the bifunctional xylan hydrolase in the NCBI database is wp014043093. 3) Substrate pretreatment: The corncob was crushed, dried, passed through a 60-mesh sieve and weighed. 4) enzymatic reaction: 10ml of the enzyme catalytic system was carried out in a closed container of 50ml. Weigh 10% wt corncob as substrate, add 300U/mL bifunctional xylan hydrolase. At pH 7.0,90 °c water bath, shaking speed 300RMP, reaction 24h. To the reaction solution obtained above, 200U/mL of a bifunctional xylan hydrolase was added, and the reaction was carried out at a water bath Shaker at 75 ° C. At a rotation speed of 200RMP for 24 hours, followed by centrifugation to obtain an enzymatic supernatant containing xylooligosaccharides. The same method was performed with the inactivated enzyme solution as a blank control. 5) product analysis: by HPLC analysis, the product contains 324mg xylooligosaccharide, of which xylobiose 97mg, xylotriose 178mg and xylotetraose 49mg. |
trait | xylooligosaccharides as crystals are colorless to light brown liquid or white solid. Syrup is a light yellow viscous liquid. The relative sweetness is about 40%, and the sweet feeling is the same as that of sucrose. Heat-resistant, acid-resistant (pH 2.5-8.0,120 ℃,1H), no damage, no change at pH 2.5-8.0 and 37 ℃ for 90 days. Use of xylooligosaccharides: Sweeteners, nutritional additives. |
Introduction | xylooligosaccharides, also known as xylooligosaccharides, is composed of 2-7 xylose molecules in order to beta -1,4 glycosidic bond combined into a functional polymeric sugar. Compared with soybean oligosaccharides, fructooligosaccharides, isomaltooligosaccharides and the like commonly used by people, it has a unique advantage that it can selectively promote the proliferative activity of intestinal Bifidobacterium. Its bifidobacteria factor function is 10-20 times that of other polymerized saccharides. |
Use | sweetener, nutritional additive. Properties: colorless to light brown liquid or white powder. A mixture of disaccharides to heptasaccharides bound by 2-7 xylose in beta -1, 4-glycosidic linkages. Xylobiose accounted for about 40%. Syrup is a light yellow viscous liquid. The relative sweetness is about 40%, and the sweet feeling is the same as that of sucrose. Heat-resistant, acid-resistant (pH 2.5-8.0,120 ℃,1H), no damage, no change in the pH 2.5-8.0 and 37 ℃ 90 days storage purposes: biochemical research. Sweeteners |
principle of action | 1. Promote the reproduction of beneficial bacteria in the digestive tract of animals, inhibit the growth of harmful bacteria, and improve the balance of microbial flora. 2. Blocking the binding of pathogenic bacteria to host cell receptors. 3. Stimulate the animal's immune system and improve immunity. 4. Enhance animal disease resistance, reduce Incidence Rate, improve the effect of prevention and treatment of animal diseases. 5. Improve feed utilization, improve animal production performance. 6. Reduce environmental pollution and improve the health status of animal production environment. |
consumption | ≤ 3.0g/day (based on xylobiose-xyloheptaose) |
applicable food category | the scope of use does not include infant food. |
approval date | 2014-12-19 |
Announcement No. | Health and Family Planning Commission 20th in 2014 |
Title of announcement | announcement on approval of 9 new food ingredients such as tomato seed oil |
Trait | light yellow viscous liquid |
Xylooligosaccharides (xylobiose-xyloheptaose) content (on dry basis)(g/100g) | ≥70 |
Xylobiose-xylotetraose content (on dry basis)(g /100g) | ≥50 |
Dry Matter (solid)(g/100g) | 70±1 |
pH | 3.5-6.5 |
Ash (g/100g) | ≤0.3 |